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Home / Equine Research Bank / Cytotherapy / Osteogenic comparison of expanded and uncultured adipose str...
Cytotherapy2010; 12(4); 554-562; doi: 10.3109/14653241003709694

Osteogenic comparison of expanded and uncultured adipose stromal cells.

Abstract: Adipose stromal cells (ASC) are a promising alternative to progenitor cells from other tissue compartments because of their multipotential and capacity to retrieve significantly more progenitor cells. Initial cell samples are heterogeneous, containing a collection of cells that may contribute to tissue repair, but the sample becomes more homogeneous with each passage. Therefore, we hypothesized that the osteogenic potential of culture-expanded ASC would differ from uncultured ASC. Methods: Adipose tissue was collected from a yearling colt, and ASC were isolated and expanded using standard protocols or prepared by a commercial vendor using proprietary technology (proprietary stromal vascular fraction, SVFp). Cells were seeded on collagen sponges and maintained in osteogenic culture conditions for up to 21 days to assess osteogenic potential. The ability of each population to stimulate neovascularization and bone healing was determined upon implanting cell-loaded sponges into a rodent calvarial bone defect. Neovascularization was measured 3 weeks post-implantation, while bone formation was monitored over 12 weeks using in vivo microcomputed tomography (microCT). Results: SVFp exhibited increased intracellular alkaline phosphatase activity compared with cultured ASC but proliferated minimally. Histologic analysis of explanted tissues demonstrated greater vascularization in defects treated with cultured ASC compared with SVFp. We detected increases in bone volume for defects treated with cultured cells while observing similar values for bone mineral density, regardless of cell type. Conclusions: These results suggest that expanded ASC are advantageous for neovascularization and bone healing in this model compared with SVFp, and provide additional evidence of the utility of ASC in bone repair.
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Publication Date: 2010-04-08 PubMed ID: 20370353DOI: 10.3109/14653241003709694Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

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The research article focuses on a study comparing the osteogenic potential of adipose stromal cells (ASC) that have been culture-expanded with that of uncultured ASC, with results denoting the former as more effective in fostering neovascularization and bone healing.

Background & Hypothesis

  • Adipose stromal cells (ASC) present an appealing alternative to progenitor cells drawn from other tissue compartments due to their multipotential nature and ability to yield more progenitor cells.
  • The researchers note that initial ASC samples are heterogeneous, meaning they contain multiple cell types. However, with each cell culture passage, the sample tends towards homogeneity.
  • Due to the shift in composition during the culture expansion, the researchers hypothesize that the osteogenic (bone formation) potential of the cultured ASC will differ from that of the uncultured ASC.

Methodology

  • The study used adipose tissue from a young horse and isolated ASC to be expanded by standard protocols or by a commercial vendor’s proprietary technique, termed proprietary stromal vascular fraction (SVFp).
  • The cultured and uncultured ASC were seeded onto collagen sponges and retained in osteogenic culture conditions for 21 days to measure osteogenic potential.
  • The measure of neovascularization (new blood vessel formation) and bone healing in response to each population was assessed by implanting the cell-infused sponges into a bone defect in a rodent skull.
  • Neovascularization was evaluated 3 weeks post-implantation, and the bone formation process was monitored over 12 weeks, using in vivo microcomputed tomography (microCT).

Results

  • The SVFp cells showed increased intracellular alkaline phosphatase activity compared to cultured ASC but had minimal proliferation.
  • On analysing the explanted tissues, it was ascertained that the areas treated with cultured ASC demonstrated superior vascularization versus the SVFp-treated areas.
  • Increases in bone volume were noted in defects treated with cultured cells, with comparable bone mineral density values observed for both cell types.

Conclusions

  • The study’s findings highlight that cultured ASC proves more beneficial for neovascularization and bone healing in comparison to SVFp, in the examined model.
  • This further reinforces the potential of ASC to contribute to bone repair processes and advancements in related treatments and therapies.

Cite This Article

APA
Cheung WK, Working DM, Galuppo LD, Leach JK. (2010). Osteogenic comparison of expanded and uncultured adipose stromal cells. Cytotherapy, 12(4), 554-562. https://doi.org/10.3109/14653241003709694

Publication

Cytotherapy
ISSN: 1477-2566
NlmUniqueID: 100895309
Country: England
Language: English
Volume: 12
Issue: 4
Pages: 554-562

Researcher Affiliations

Cheung, Whitney K
  • Department of Biomedical Engineering, School of Veterinary Medicine, University of California, Davis, California 95616, USA.
Working, David M
    Galuppo, Larry D
      Leach, J Kent

        MeSH Terms

        • Animals
        • Calcification, Physiologic
        • Cell Proliferation
        • Cells, Cultured
        • Horses
        • Male
        • Mesenchymal Stem Cell Transplantation
        • Models, Animal
        • Neovascularization, Physiologic
        • Osteogenesis
        • Rats
        • Rats, Nude
        • Skull / blood supply
        • Skull / growth & development
        • Skull / pathology
        • Skull / surgery
        • Skull Fractures / pathology
        • Skull Fractures / therapy
        • Stem Cells / cytology
        • Stem Cells / physiology
        • Stromal Cells / cytology
        • Stromal Cells / physiology
        • Subcutaneous Fat / cytology
        • Transplantation, Heterologous

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