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Immunology1974; 27(2); 271-283;

Passive cutaneous anaphylaxis and its enhancement by normal IgG.

Abstract: Rats were injected intradermally with rabbit anti-ovalbumin serum and 3 hours later were challenged intravenously with ovalbumin and Evans Blue dye. Inflammatory lesions were produced within 20 minutes and their size was markedly dose-dependent. Attempts were made to interfere with this passive cutaneous anaphylaxis (PCA) by admixture of normal IgG with the rabbit anti-ovalbumin to measure the relative tissue binding affinities of IgG from various species. It was found that normal IgG from any of the species tested had an enhancing effect on PCA in rats. These immunoglobulins serially arranged in the order of their decreasing enhancing abilities were: bovine>guinea-pig>pig>rat>rabbit>horse>human. The extent and significance of the enhancement increased considerably with increasing concentration of IgG in the test doses. The mechanism of enhancement was investigated and a hypothesis was proposed to explain the phenomenon.
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Publication Date: 1974-08-01 PubMed ID: 4421606PubMed Central: PMC1445577
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research study investigated the role of normal immunoglobulin G (IgG) in enhancing passive cutaneous anaphylaxis, a type of allergic reaction, in rats when combined with a specific anti-allergen serum. Researchers concluded that IgG from different species can, indeed, enhance allergic reactions and that the extent of this enhancement depends on the concentration of IgG used and the species it originates from.

Study Design and Methods

  • The experiment used rats as subjects. To simulate an allergic reaction, the researchers injected the rats’ skin with anti-ovalbumin serum from rabbits, then injected the rats with ovalbumin (a protein commonly found in egg whites) and Evans Blue dye.
  • The inflammation caused by the ovalbumin was tracked using the dye, which stained the inflamed areas. The researchers then monitored and measured the size of these inflammatory lesions.
  • The team studied the severity and size of the allergic reaction by varying the dose of the ovalbumin challenge. They observed that the size of the inflammation was dose-dependent, changing in direct correlation with the ovalbumin dose.

Role of Normal IgG

  • Attempts were made to interfere with the allergic reaction process, by admixing normal IgG (an antibody that the immune system uses to neutralize bacteria and viruses) with the rabbit anti-ovalbumin.
  • The team did this to measure the relative tissue binding affinities of IgG from different species. In other words, they wanted to find out how well IgG from different species could stick to the inflamed tissues in the rats.
  • The finding was that normal IgG from any of the species tested enhanced the allergic reaction in rats. This means that normal IgG, when combined with an antigen like ovalbumin, can actually exacerbate allergic reactions rather than alleviating them.

The Order of Enhancing Abilities and Mechanism

  • The study revealed that IgG from different species had different effects on allergic reactions, with some having a stronger enhancing effect than others.
  • The researchers found that IgG from bovines (cows) had the strongest effect, followed by guinea pigs, pigs, rats, rabbits, horses, and humans having the weakest effect.
  • The extent and significance of the enhancement increased with the increasing concentrations of IgG in the doses given to the rats.
  • In terms of the underlying mechanism, the researchers proposed a hypothesis explaining the phenomenon, although the abstract does not elaborate on what this hypothesis is.

In conclusion, this study provides significant insights into how normal IgG can enhance allergic reactions, and raises important questions about how different IgGs from different species can modulate these reactions. Such understandings could be crucial in developing new therapeutic strategies to manage allergic reactions.

Cite This Article

APA
Williams MR. (1974). Passive cutaneous anaphylaxis and its enhancement by normal IgG. Immunology, 27(2), 271-283.

Publication

Immunology
ISSN: 0019-2805
NlmUniqueID: 0374672
Country: England
Language: English
Volume: 27
Issue: 2
Pages: 271-283

Researcher Affiliations

Williams, M R

    MeSH Terms

    • Animals
    • Cattle / immunology
    • Guinea Pigs / immunology
    • Histamine
    • Horses / immunology
    • Hot Temperature
    • Immune Sera
    • Immunoelectrophoresis
    • Immunoglobulin G / isolation & purification
    • Nitrogen / analysis
    • Ovalbumin / immunology
    • Passive Cutaneous Anaphylaxis
    • Rabbits / immunology
    • Rats / immunology
    • Swine / immunology

    References

    This article includes 17 references
    1. OVARY Z, BLOCH KJ, BENACERRAF B. IDENTIFICATION OF RABBIT, MONKEY, AND DOG ANTIBODIES WITH PCA ACTIVITY FOR GUINEA PIGS.. Proc Soc Exp Biol Med 1964 Jul;116:840-5.
      pubmed: 14194674doi: 10.3181/00379727-116-29388google scholar: lookup
    2. Lovett CA, Movat HZ. Role of PMN-leukocyte lyosomes in tissue injury, inflammation and hypersensitivity. 3. Passive cutaneous anaphylaxis induced in the rat with homologous and heterologous hyperimmune antibody.. Proc Soc Exp Biol Med 1966 Aug-Sep;122(4):991-6.
      pubmed: 4380596doi: 10.3181/00379727-122-31307google scholar: lookup
    3. HARTLEY P. The effect of peptic digestion on the properties of diphtheria antitoxin.. Proc R Soc Lond B Biol Sci 1951 Oct 30;138(893):499-513.
      pubmed: 14911806doi: 10.1098/rspb.1951.0037google scholar: lookup
    4. Orr TS, Gwilliam J, Cox JS. Studies on passive cutaneous anaphylaxis in the rat with disodium cromoglycate. II. A comparison of the rat anti-dnp 7S 2 and rat reagin induced cutaneous reactions.. Immunology 1971 Sep;21(3):405-17.
      pubmed: 4998629
    5. Kekwick RA. The serum proteins in multiple myelomatosis.. Biochem J 1940 Sep;34(8-9):1248-57.
      pubmed: 16747310doi: 10.1042/bj0341248google scholar: lookup
    6. FISHER JP, COOKE RA. Passive cutaneous anaphylaxis (PCA) in the guinea pig; an immunologic and pathologic study.. J Allergy 1957 Mar;28(2):150-69.
      pubmed: 13405691doi: 10.1016/0021-8707(57)90021-7google scholar: lookup
    7. Webb T, Goodman HC. The structure and function of immunoglobulins.. Mod Trends Immunol 1967;2:151-87.
      pubmed: 4172716
    8. ISHIZAKA K, ISHIZAKA T. Biologic activity of aggregated gamma-globulin. II. A study of various methods for aggregation and species differences.. J Immunol 1960 Aug;85:163-71.
      pubmed: 13718021
    9. King CA, Francis GE. Adsorption of heterologous gamma-globulins by chopped guinea-pig lung.. Immunology 1967 Jul;13(1):1-8.
      pubmed: 4165631
    10. MOTA I. Mast cells and anaphylaxis.. Ann N Y Acad Sci 1963 Feb 26;103:264-77.
      pubmed: 13936245doi: 10.1111/j.1749-6632.1963.tb53703.xgoogle scholar: lookup
    11. ALUND O, OSEBOLD JW, MURPHY FA. ISOLATION AND CHARACTERIZATION OF OVINE GAMMA GLOBULINS.. Arch Biochem Biophys 1965 Jan;109:142-9.
      pubmed: 14281939doi: 10.1016/0003-9861(65)90299-7google scholar: lookup
    12. Movat HZ, Lovett CA, Taichman NS. Demonstration of antigen on the surface of sensitized rat mast cells.. Nature 1966 Nov 19;212(5064):851-3.
      pubmed: 4383712doi: 10.1038/212851b0google scholar: lookup
    13. BRAMBELL FW, HEMMINGS WA, MORRIS IG. A THEORETICAL MODEL OF GAMMA-GLOBULIN CATABOLISM.. Nature 1964 Sep 26;203:1352-4.
      pubmed: 14207307doi: 10.1038/2031352a0google scholar: lookup
    14. BIOZZI G, HALPERN BN, BINAGHI R. The competitive effect of normal serum proteins from various animal species on antibody fixation in passive cutaneous anaphylaxis in the guinea pig.. J Immunol 1959 Mar;82(3):215-8.
      pubmed: 13631253
    15. Goose J, Blair AM. Passive cutaneous anaphylaxis in the rat, induced with two homologous reagin-like antibodies and its specific inhibition with disodium cromoglycate.. Immunology 1969 Jun;16(6):749-60.
      pubmed: 4306785
    16. SPIEGELBERG HL, WEIGLE WO. THE CATABOLISM OF HOMOLOGOUS AND HETEROLOGOUS 7S GAMMA GLOBULIN FRAGMENTS.. J Exp Med 1965 Mar 1;121(3):323-38.
      pubmed: 14271318doi: 10.1084/jem.121.3.323google scholar: lookup
    17. OVARY Z. [Cutaneous anaphylaxis in the albino rat].. Int Arch Allergy Appl Immunol 1952;3(4):293-301.
      pubmed: 13044324doi: 10.1159/000227977google scholar: lookup

    Citations

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