Pre-analytical stability of selected biochemical analytes in serum of horses and oxen stored at -20°C.
Abstract: Delays between blood collection and analysis are inevitable, and samples are always stored in the refrigerator. The current study aimed to evaluate the stability of serum total cholesterol (TC), triglycerides (TG), total protein (TP), albumin and urea (URA) in horses and oxen after storage at -20°C. Methods: Sera from apparently healthy 20 male horses and 20 oxen were obtained and aliquots of serum were divided into 3 portions. The first tube was used for baseline (T0) measurement of analyte values, whereas the other two tubes, T1 and T2, were stored at -20°C for 1 and 2 months, respectively, and analyte measurement was done. Results: Results showed that the stability of TP (g/dL), URA (mg/dL) and TC (mg/dL) in oxen was statistically significant (p < 0.05). In horses, the stability of URA (mg/dL), TP (g/dL) and TG (mg/dL) were also statistically significant (p < 0.05). Additionally, URA and TC in oxen exceed TE following measurement at T2 and TG in horses following measurement at T1 and T2. Conclusions: Laboratories should consider the storage temperature and time for specific analytes among animals. Therefore, stability studies at various storage temperatures and times are recommended to fully validate the stability of the analytes.
© 2024 The Authors. Veterinary Medicine and Science published by John Wiley & Sons Ltd.
Publication Date: 2024-02-15 PubMed ID: 38356378PubMed Central: PMC10867542DOI: 10.1002/vms3.1368Google Scholar: Lookup
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- Journal Article
Summary
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The research explores the impact of storage temperature and duration on the stability of certain biochemical substances in the blood of horses and oxen. Specifically, the study found that the levels of total cholesterol, triglycerides, total protein, albumin, and urea show significant changes after being stored at -20°C for one to two months.
Research Methodology
- The research involved blood samples from 20 healthy male horses and 20 oxen.
- Each blood sample was divided into three smaller portions for different tests, with one portion being used for immediate baseline measurements (T0).
- The second and third batches (T1 and T2) were frozen at -20°C for one and two months, respectively, before undergoing the same measurements.
Key Findings
- For oxen samples, the stability of total protein, urea, and total cholesterol showed statistically significant variations after storage.
- For horse samples, significant changes were observed in the stability of urea, total protein, and triglycerides.
- For some oxen samples, the Urea and Total Cholesterol levels exceeded the Total Error following the two-month storage point.
- In the case of some horses, Triglycerides levels also surpassed the total error after measurements at both the one and two-month storage checkpoints.
Conclusions and Recommendations
- The findings emphasize that laboratories should take into account the storage temperature and time for specific blood analytes while working with animal samples.
- The study also suggests further stability studies at various storage temperatures and periods, which would lead to a more comprehensive understanding of the stability of these analytes.
Cite This Article
APA
Megerssa YC, Gari FR.
(2024).
Pre-analytical stability of selected biochemical analytes in serum of horses and oxen stored at -20°C.
Vet Med Sci, 10(2), e1368.
https://doi.org/10.1002/vms3.1368 Publication
Researcher Affiliations
- Department of Biomedical Sciences, College of Veterinary Medicine and Agriculture, Addis Ababa University, Bishoftu, Ethiopia.
- Department of Biomedical Sciences, College of Veterinary Medicine and Agriculture, Addis Ababa University, Bishoftu, Ethiopia.
MeSH Terms
- Male
- Horses
- Animals
- Blood Specimen Collection / methods
- Blood Specimen Collection / veterinary
- Serum
- Triglycerides
- Time Factors
- Temperature
Conflict of Interest Statement
The authors declare that no conflicts of interest to declare.
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