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Veterinary world2023; 16(10); 2128-2134; doi: 10.14202/vetworld.2023.2128-2134

Preliminary study on prevalence of hemoprotozoan parasites harbored by Stomoxys (Diptera: Muscidae) and tabanid flies (Diptera: Tabanidae) in horse farms in Nakhon Si Thammarat province, Southern Thailand.

Abstract: and tabanid flies are of medical and veterinary importance because they play crucial roles in disease transmission as mechanical vectors of various hemopathogens. However, its role as a hemoprotozoan parasite vector in horse farms has not been studied. Therefore, we investigated the occurrence of hemoprotozoan parasites belonging to the genera , , and in and tabanid flies using conventional polymerase chain reaction (PCR) and DNA sequencing. Unassigned: All samples ( and tabanid flies) were collected using an Nzi trap for three consecutive days each month from November 2022 to March 2023. The flies were morphologically identified to the species level and separated according to sex. Individual (for tabanid flies) or pooled samples (consisting of three specimens of flies of the same species and sex collected from the same site) were used for DNA extraction. Conventional PCR was used to screen for hemoprotozoan parasite DNA, followed by Sanger sequencing to identify the species. Unassigned: In total, 189 biting flies were collected, including four species of (, , , and ) and five species of tabanids (, , , , and ). was the most prevalent species, accounting for 58.7% (n = 111) of the collected flies. Ten (12.4%) of the 81 samples (individuals and pools) analyzed by PCR were positive for the 18S rRNA gene of the / species. DNA was not detected in any sample. After performing Basic Local Alignment Search Tool searches and a phylogenetic analysis, only six samples (7.4%), including (n = 2), (n = 2), (n = 1), and (n = 1), were found to be infected with . Furthermore, apicomplexan parasites, namely, spp. and spp., were found on , the fungus spp. was found on , and the pathogenic green alga spp. was found on . Unassigned: This study is the first to report a variety of and tabanid flies collected from horse farms in Thailand, which were found to be infected with and species that affect mammals, suggesting that and tabanid flies can be used to confirm the presence of hemoprotozoan parasites in the study area. Understanding the presence of hemoprotozoa in flies could help design vector control programs and manage various diseases in the study area.
Copyright: © Phetkarl, et al.
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Publication Date: 2023-10-18 PubMed ID: 38023282PubMed Central: PMC10668551DOI: 10.14202/vetworld.2023.2128-2134Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates the presence and role of hemoprotozoan parasites in Stomoxys and tabanid flies in horse farms in Southern Thailand, revealing that these flies play a significant role in transmitting diseases.

Objective and Methodology

  • The study aimed to explore the role of Stomoxys and tabanid flies in the transmission of hemoprotozoan parasites in horse farms in Nakhon Si Thammarat province of Southern Thailand.
  • This was done using a polymerase chain reaction (PCR) to identify the presence of hemoprotozoan parasites, followed by DNA sequencing to identify their species.
  • Over a period of five months, samples were collected from the flies using a trapping method. The flies were then identified and separated based on the species and sex.

Data Collection and Results

  • A total of 189 biting flies were gathered. They represented four species of Stomoxys and five species of tabanid flies.
  • They found hemoprotozoan parasite DNA in ten out of 81 samples tested, but no DNA from Trypanosoma species was found in any sample.
  • Additionally, through alignment searches and phylogenetic analysis, six samples were confirmed to be infected with Theileria equi. The infected samples included two each from two species of Stomoxys, and one each from two species of tabanids.
  • Moreover, a variety of parasites such as gregarines and coccidia were detected on various species of the flies, in addition to finding fungal and algal species.

Conclusion and Implications

  • This research is the first to examine and report the presence of hemoprotozoan parasites in Stomoxys and tabanid flies from horse farms in Thailand.
  • The findings suggest these flies are carriers of Theileria equi, one of the hemoprotozoan parasites that affect mammals, notably horses.
  • The knowledge gained through this study will potentially aid in designing effective vector control programs, thus managing various diseases in the region.

Cite This Article

APA
Phetkarl T, Fungwithaya P, Udompornprasith S, Amendt J, Sontigun N. (2023). Preliminary study on prevalence of hemoprotozoan parasites harbored by Stomoxys (Diptera: Muscidae) and tabanid flies (Diptera: Tabanidae) in horse farms in Nakhon Si Thammarat province, Southern Thailand. Vet World, 16(10), 2128-2134. https://doi.org/10.14202/vetworld.2023.2128-2134

Publication

Veterinary world
ISSN: 0972-8988
NlmUniqueID: 101504872
Country: India
Language: English
Volume: 16
Issue: 10
Pages: 2128-2134

Researcher Affiliations

Phetkarl, Tanakorn
  • Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat, 80160, Thailand.
Fungwithaya, Punpichaya
  • School of Agricultural Technology, King Mongkut's Institute of Technology Ladkrabang, Bangkok, 10520, Thailand.
Udompornprasith, Supak
  • Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat, 80160, Thailand.
Amendt, Jens
  • Institute of Legal Medicine, University Hospital Frankfurt, Goethe-University, Kennedyallee 104, 60596, Frankfurt am Main, Germany.
Sontigun, Narin
  • Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat, 80160, Thailand.
  • One Health Research Center, Walailak University, Nakhon Si Thammarat, 80160, Thailand.
  • Center of Excellence Research for Melioidosis and Microorganisms, Walailak University, Nakhon Si Thammarat, 80160, Thailand.

Conflict of Interest Statement

The authors declare that they have no competing interests.

References

This article includes 41 references
  1. Baldacchino F, Muenworn V, Desquesnes M, Desoli F, Charoenviriyaphap T, Duvallet G. Transmission of pathogens by Stomoxys flies (Diptera, Muscidae):A review.. Parasite 2013;20:26.
    pmc: PMC3756335pubmed: 23985165
  2. Baldacchino F, Desquesnes M, Mihok S, Foil L.D, Duvallet G, Jittapalapong S. Tabanids:Neglected subjects of research, but important vectors of disease agents!. Infect. Genet. Evol. 2014;28:596–615.
    pubmed: 24727644
  3. Taioe M.O, Motloang M.Y, Namangala B, Chota A, Molefe N.I, Musinguzi S.P, Suganuma K, Hayes P, Tsilo T.J, Chainey J, Inoue N, Thekisoe O.M.M. Characterization of tabanid flies (Diptera:Tabanidae) in South Africa and Zambia and detection of protozoan parasites they are harbouring.. Parasitology 2017;144(9):1162–1178.
    pubmed: 28502276
  4. Odeniran P.O, Macleod E.T, Ademola I.O, Welburn S.C. Molecular identification of bloodmeal sources and trypanosomes in Glossina spp, Tabanus spp. and Stomoxys spp. Trapped on cattle farm settlements in southwest Nigeria.. Med. Vet. Entomol. 2019;33(2):269–281.
    pubmed: 30730048
  5. Hornok S, Takács N, Szekeres S, Szőke K, Kontschán J, Horváth G, Sugár L. DNA of Theileria orientalis, T. equi and T. capreoli in stable flies (Stomoxys calcitrans). Parasit. Vectors 2020;13(1):186.
    pmc: PMC7144340pubmed: 32272968
  6. Williams P. Studies on Ethiopian Chrysops as possible vectors of loiasis. II. Chrysops silacea Austen and human loiasis.. Ann. Trop. Med. Parasitol. 1960;54:439–459.
    pubmed: 13785485
  7. Traversa D, Otranto D, Iorio R, Carluccio A, Contri A, Paoletti B, Bartolini R, Giangaspero A. Identification of the intermediate hosts of Habronema microstoma and Habronema muscae under field conditions.. Med. Vet. Entomol. 2008;22(3):283–287.
    pubmed: 18816277
  8. Ganyukova A.I, Zolotarev A.V, Malysheva M.N, Frolov A.O. First record of Trypanosoma theileri-like flagellates in horseflies from Northwest Russia.. Protistology 2018;12:223–230.
  9. Kamyingkird K, Yangtara S, Desquesnes M, Cao S, Adjou Moumouni P.K, Jittapalapong S, Nimsupan B, Terkawi M.A, Masatani T, Nishikawa Y, Igarashi I, Xuan X. Seroprevalence of Babesia caballi and Theileria equi in horses and mules from Northern Thailand.. J. Protozool. Res. 2014;24:11–17.
  10. Ereqat S, Nasereddin A, Al-Jawabreh A, Al-Jawabreh H, Al-Laham N, Abdeen Z. Prevalence of Trypanosoma evansi in livestock in Palestine.. Parasit. Vectors 2020;13(1):21.
    pmc: PMC6958583pubmed: 31931864
  11. Rakwong P, Keawchana N, Ngasaman R, Kamyingkird K. Theileria infection in bullfighting cattle in Thailand.. Vet. World. 2021;15(12):2917–2921.
    pmc: PMC9880834pubmed: 36718341
  12. Hossain M.J, Raut S, Singh R.P, Mishra P, Hossain M.S, Dey A.R, Kabir A, Anisuzzaman Talukder M.H, Shahiduzzaman M. Molecular detection of Babesia and Theileria from crossbred cattle in Sirajganj and Rangpur districts of Bangladesh.. Vet. Med. Sci. 2023;9(2):899–906.
    pmc: PMC10029904pubmed: 36331989
  13. Changbunjong T, Sedwisi P, Weluwanarak T, Nitiyamatawat E, Sariwongchan R, Chareonviriyaphap T. Species diversity and abundance of Tabanus spp. (Diptera:Tabanidae) in different habitats of Thailand.. J. Asia Pac. Entomol. 2018;21(1):134–139.
  14. Lorn S, Ratisupakorn S, Duvallet G, Chareonviriyaphap T, Tainchum K. Species composition and abundance of Stomoxys spp. (Diptera:Muscidae) in Peninsular Thailand.. J. Med. Entomol. 2020;57(1):252–258.
    pubmed: 31349364
  15. Mantiantipan T, Chibangyang N, Weluwanarak T, Sedwisai P, Changbunjong T. A survey of Stomoxys spp. (Diptera:Muscidae) at horse stable of faculty of veterinary science, Mahidol University, Nakhon Pathom Province.. J. Appl. Anim. Sci. 2014;7(1):43–51.
  16. Poolkhetkit S, Chowattanapon W, Sungpradit S. Molecular detection of blood protozoa in ticks collected from cattle in the buffer zone of Sai Yok National Park, Thailand.. Thai J. Vet. Med. 2015;45(4):619–625.
  17. Jirapattharasate C, Adjou Moumouni P.F, Cao S, Iguchi A, Liu M, Wang G, Zhou M, Vudriko P, Efstratiou A, Changbunjong T, Sungpradit S, Ratanakorn P, Moonarmart W, Sedwisai P, Weluwanarak T, Wongsawang W, Suzuki H, Xuan X. Molecular detection and genetic diversity of bovine Babesia spp., Theileria orientalis, and Anaplasma marginale in beef cattle in Thailand.. Parasitol. Res. 2017;116(2):751–762.
    pubmed: 28028631
  18. Changbunjong T, Sungpradit S, Kanthasaewee O, Sedwisai P, Tangsudjai S, Ruangsittichai J. Molecular detection of Theileria and Babesia in a diversity of Stomoxyini flies (Diptera:Muscidae) from Khao Yai National Park, Thailand.. Thai J. Vet. Med. 2016;46(2):227–234.
  19. Jirapattharasate C, Changbunjong T, Sedwisai P, Weluwanarak T. Molecular detection of piroplasms in haematophagus flies in the Nakhon Pathom and Kanchanaburi Provinces, Thailand.. Vet. Integr Sci. 2018;16(2):123–133.
  20. Sontigun N, Boonhoh W, Phetcharat Y, Wongtawan T. First study on molecular detection of hemopathogens in tabanid flies (Diptera:Tabanidae) and cattle in Southern Thailand.. Vet.World. 2022;15(8):2089–2094.
    pmc: PMC9615497pubmed: 36313830
  21. Mihok S. The development of a multipurpose trap (the Nzi) for tsetse and other biting flies.. Bull. Entomol. Res. 2002;92(5):385–403.
    pubmed: 12241564
  22. Burton J.J.S. Tabanini of Thailand above the Isthmus of Kra (Diptera:Tabanidae). Los Angeles: Entomological Reprint Specialists; 1978. p. 165.
  23. Tumrasvin W, Shinonaga S. Studies on medically important flies in Thailand. V. On 32 species belonging to the subfamilies Muscinae and Stomoxyinae including the taxonomic keys (Diptera:Muscidae). Bull. Tokyo Med. Dent. Univ. 1978;25(4):201–227.
    pubmed: 282017
  24. Burger J.F, Chainey J.E. Revision of the oriental and Australasian species of Chrysops (Diptera:Tabanidae). Invertebr. Taxon. 2000;14:607–654.
  25. Kledmanee K, Suwanpakdee S, Krajangwong S, Chatsiriwech J, Suksai P, Suwannachat P, Sariya L, Buddhirongawatr R, Charoonrut P, Chaichoun K. Development of multiplex polymerase chain reaction for detection of Ehrlichia canis, Babesia spp and Hepatozoon canis in canine blood.. Southeast Asian J. Trop. Med. Public Health. 2009;40(1):35–39.
    pubmed: 19323031
  26. Njiru Z.K, Constantine C.C, Guya S, Crowther J, Kiragu J.M, Thompson R.C, Dávila A.M. The use of ITS1 rDNA PCR in detecting pathogenic African trypanosomes.. Parasitol. Res. 2005;95(3):186–192.
    pubmed: 15619129
  27. Hall T.A. BioEdit:A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT.. Nucl. Acids. Symp. Ser. 1999;41:95–98.
  28. Tamura K, Nei M. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees.. Mol. Biol. Evol. 1993;10(3):512–526.
    pubmed: 8336541
  29. Kumar S, Stecher G, Li M, Knyaz C, Tamura -K. MEGA X:Molecular Evolutionary Genetics Analysis across computing platforms.. Mol. Biol. Evol. 2018;35(6):1547–1549.
    pmc: PMC5967553pubmed: 29722887
  30. Muenworn V, Duvallet G, Thainchum K, Tuntakom S, Tanasilchayakul S, Prabaripai A, Akratanakul P, Sukonthabhirom S, Chareonviriyaphap T. Geographic distribution of stomoxyine flies (Diptera:Muscidae) and diurnal activity of Stomoxys calcitrans in Thailand.. J. Med. Entomol. 2010;47(5):791–797.
    pubmed: 20939373
  31. Changbunjong T, Weluwanarak T, Ratanakorn P, Maneeon P, Ganpanakngan M, Apiwathnasorn C, Sungvornyothin S, Sriwichai P, Sumruayphol S, Ruangsittichai J. Distribution and abundance of Stomoxyini flies (Diptera:Muscidae) in Thailand.. Southeast Asian J. Trop. Med. Public Health. 2012;43(6):1400–1410.
    pubmed: 23413703
  32. Bai Q, Liu G, Yin H, Zhao Q, Liu D, Ren J. Theileria sinensis sp nov:A new species of bovine Theileria-molecular taxonomic studies.. Xu Mu Shou Yi Xue Bao 2002;33(2):185–190.
  33. Bursakov S.A, Kovalchuk S.N. Co-infection with tick-borne disease agents in cattle in Russia.. Ticks Tick Borne Dis. 2019;10(3):709–713.
    pubmed: 30878569
  34. Agina O.A, Shaari M.R, Isa N.M.M, Ajat M, Zamri-Saad M, Mazlan M, Muhamad A.S, Kassim A.A, Ha L.C, Rusli F.H, Masaud D, Hamzah H. Molecular detection of Theileria species, Anaplasma species, Candidatus Mycoplasma haemobos, Trypanosoma evansi and first evidence of Theileria sinensis-associated bovine anaemia in crossbred Kedah-Kelantan x Brahman cattle.. BMC Vet. Res. 2021;17(1):246.
    pmc: PMC8286590pubmed: 34275459
  35. Chen Y, Chen Y.Y, Liu G, Lyu C, Hu Y, An Q, Qiu H.Y, Zhao Q, Wang C.R. Prevalence of Theileria in cattle in China:A systematic review and meta-analysis.. Microb. Pathog. 2022;162:105369.
    pubmed: 34952152
  36. Kuvardina O.N, Leander B.S, Aleshin V.V, Myl'nikov A.P, Keeling P.J, Simdyanov T.G. The phylogeny of colpodellids (Alveolata) using small subunit rRNA gene sequences suggests they are the free-living sister group to apicomplexans.. J. Eukaryot. Microbiol. 2002;49(6):498–504.
    pubmed: 12503687
  37. Yuan C.L, Keeling P.J, Krause P.J, Horak A, Bent S, Rollend L, Hua X.G. Colpodella spp.-like parasite infection in woman, China.. Emerg. Infect. Dis. 2012;18(1):125–127.
    pmc: PMC3310105pubmed: 22260904
  38. Jiang J.F, Jiang R.R, Chang Q.C, Zheng Y.C, Jiang B.G, Sun Y, Jia N, Wei R, Liu H.B, Huo Q.B, Wang H, von Fricken M.E, Cao W.C. Potential novel tick-borne Colpodella species parasite infection in patient with neurological symptoms.. PLoS Negl. Trop. Dis. 2018;12(8):e0006546.
    pmc: PMC6071948pubmed: 30071019
  39. Matsimbe A.M, Magaia V, Sanches G.S, Neves L, Noormahomed E, Antunes S, Domingos A. Molecular detection of pathogens in ticks infesting cattle in Nampula Province, Mozambique.. Exp. Appl. Acarol. 2017;73(1):91–102.
    pmc: PMC5705812pubmed: 28856544
  40. Chiu H.C, Sun X, Bao Y, Fu W, Lin K, Chen T, Zheng C, Li S, Chen W, Huang C. Molecular identification of Colpodella spp. of South China tiger Panthera tigris amoyensis (Hilzheimer) in the Meihua Mountains, Fujian, China.. Folia Parasitol (Praha) 2022;69:019.
    pubmed: 36193766
  41. Xu M, Hu Y, Qiu H, Wang J, Jiang J. Colpodella spp. (Phylum Apicomplexa) identified in horses shed light on its potential transmission and zoonotic pathogenicity.. Front. Microbiol. 2022;13:857752.
    pmc: PMC9058166pubmed: 35509316

Citations

This article has been cited 8 times.
  1. Gomontean B, Wannasingha W, Jumpato W, Nabkrathok A, Jaroenchaiwattanachote C, Wongpakam K, Pramual P. Molecular Detections of Hematoparasites in Tabanids (Diptera: Tabanidae) from Northeastern Thailand. Pathogens 2025 Nov 27;14(12).
    doi: 10.3390/pathogens14121208pubmed: 41471164google scholar: lookup
  2. Ullah A, Geng M, Chen W, Zhu Q, Shi L, Zhang X, Akhtar MF, Wang C, Khan MZ. Effect of Parasitic Infections on Hematological Profile, Reproductive and Productive Performance in Equines. Animals (Basel) 2025 Nov 14;15(22).
    doi: 10.3390/ani15223294pubmed: 41302002google scholar: lookup
  3. Yang X, Dai Y, Yu J, Tang J, Chen X, Chen Q, Cao W, Wu J, Yu F, Zhan L. Detection of Colpodella sp. in blood of an ICU patient in Guizhou Province, China: a preliminary molecular report. Front Med (Lausanne) 2025;12:1638864.
    doi: 10.3389/fmed.2025.1638864pubmed: 41103706google scholar: lookup
  4. Zhao Y, Cao Z, Li S, Du C, Jiang J. Biological characteristics and epidemiological insights into the zoonotic potential of Colpodella spp.: a scoping review. Infect Dis Poverty 2025 Aug 28;14(1):91.
    doi: 10.1186/s40249-025-01361-1pubmed: 40877989google scholar: lookup
  5. Jhaiaun P, Rudeekiatthamrong A, Chimnoi W, Nguyen GT, Ngasaman R, Phasuk J, Kamyingkird K. Molecular Detection of Hemoparasites in Hematophagous Insects Collected from Livestock Farms in Northeastern Thailand. Insects 2025 Feb 14;16(2).
    doi: 10.3390/insects16020207pubmed: 40003837google scholar: lookup
  6. González CR, Reyes C, Castillo A, Valderrama L, Llanos L, Fernández J, Eastwood G, Cancino-Faure B. Molecular evidence of pathogens and endosymbionts in the black horse fly Osca lata (Diptera: Tabanidae) in Southern Chile. PLoS Negl Trop Dis 2024 Sep;18(9):e0012525.
    doi: 10.1371/journal.pntd.0012525pubmed: 39331668google scholar: lookup
  7. Weluwanarak T, Chaiphongpachara T, Changbunjong T. Evaluation of the wing cell contour to distinguish between Stomoxys bengalensis and Stomoxys sitiens (Diptera: Muscidae) using outline-based morphometrics. Curr Res Parasitol Vector Borne Dis 2024;6:100204.
    doi: 10.1016/j.crpvbd.2024.100204pubmed: 39185326google scholar: lookup
  8. Salti MI, Sam-Yellowe TY. Are Colpodella Species Pathogenic? Nutrient Uptake and Approaches to Diagnose Infections. Pathogens 2024 Jul 21;13(7).
    doi: 10.3390/pathogens13070600pubmed: 39057826google scholar: lookup
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