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Home / Equine Research Bank / Viruses / Prevalence and Sequence Analysis of Equine Rhinitis Viruses ...
Viruses2024; 16(8); 1204; doi: 10.3390/v16081204

Prevalence and Sequence Analysis of Equine Rhinitis Viruses among Horses in Poland.

Abstract: Equine rhinitis A (ERAV) and B (ERBV) viruses are respiratory pathogens with worldwide distribution. The current study aimed to determine the frequency of infection of ERAV and ERBV among horses and foals at Polish national studs, and to determine genetic variability within the viruses obtained. Virus-specific quantitative RT-PCR assays targeting a 5' untranslated region were used to screen nasal swabs collected from 621 horses at 16 national horse studs from throughout Poland, including 553 healthy horses and 68 horses with respiratory disease. A partial DNA polymerase gene was amplified and sequenced from the qRT-PCR-positive samples. The obtained sequences were analysed using phylogeny and genetic network analysis. None of the nasal swabs were positive for ERAV, whereas ERBV was found in 11/621 (1.78%) samples collected from 10 healthy horses and one foal affected by respiratory disease. Partial DNA polymerase gene sequence variability was correlated with individual horses and studs from which samples were collected when only Polish sequences were analysed, but there was no correlation between country of origin and ERBV sequence when Polish and international sequences were included in the network. The report presents the first detection of ERBV in Poland.
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Publication Date: 2024-07-26 PubMed ID: 39205178PubMed Central: PMC11359465DOI: 10.3390/v16081204Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The current research investigated the prevalence of Equine Rhinitis A (ERAV) and B (ERBV) viruses among horses in Poland by using virus-specific RT-PCR assays, analyzing the results, and sequencing the positive samples’ partial DNA polymerase gene. They found ERBV in 1.78% of the samples but none for ERAV. This marks the first detection of ERBV in Poland.

Objective of the Study

  • The main aim of this research was to determine the frequency of Equine Rhinitis A and B viruses among horses and foals in national studs across Poland.
  • The study also aimed to analyze the genetic variability in the viruses found via sequencing the partial DNA polymerase gene of qRT-PCR-positive samples.

Methodology

  • Nasal swabs were collected from 621 horses at 16 national horse studs located in various parts of Poland.
  • The sample included 553 healthy horses and 68 horses with reported respiratory diseases.
  • Virus-specific quantitative RT-PCR assays were used, targeting a 5′ untranslated region to screen these nasal swabs.
  • The positive samples from this screening were then used for amplification and sequencing of their partial DNA polymerase gene.

Findings and Analysis

  • No nasal swabs tested positive for ERAV.
  • However, ERBV was found in 11 out of the 621 samples. These included 10 healthy horses and one foal suffering from respiratory disease.
  • The sequences derived from the partial DNA polymerase gene demonstrated variability which was linked to individual horses and studs from where the samples were collected.

Conclusions

  • This study marks the first detection of ERBV in Poland, suggesting a low prevalence rate (1.78%) among horses.
  • While the gene sequence variability correlated with individual horses and studs based on their origin in Poland, no such correlation existed when Polish and international ERBV sequences were included in the network, indicating the local geographical link with the genetic variations.

Cite This Article

APA
Stasiak K, Dunowska M, Rola J. (2024). Prevalence and Sequence Analysis of Equine Rhinitis Viruses among Horses in Poland. Viruses, 16(8), 1204. https://doi.org/10.3390/v16081204

Publication

Viruses
ISSN: 1999-4915
NlmUniqueID: 101509722
Country: Switzerland
Language: English
Volume: 16
Issue: 8
PII: 1204

Researcher Affiliations

Stasiak, Karol
  • Department of Virology, National Veterinary Research Institute, 24-100 Pulawy, Poland.
Dunowska, Magdalena
  • School of Veterinary Science, Massey University, Palmerston North 4442, New Zealand.
Rola, Jerzy
  • Department of Virology, National Veterinary Research Institute, 24-100 Pulawy, Poland.

MeSH Terms

  • Horses / virology
  • Animals
  • Poland / epidemiology
  • Horse Diseases / virology
  • Horse Diseases / epidemiology
  • Picornaviridae Infections / veterinary
  • Picornaviridae Infections / virology
  • Picornaviridae Infections / epidemiology
  • Phylogeny
  • Prevalence
  • Genetic Variation
  • Erbovirus / genetics
  • Erbovirus / isolation & purification
  • Rhinovirus / genetics
  • Rhinovirus / isolation & purification
  • Rhinovirus / classification
  • Sequence Analysis, DNA

Conflict of Interest Statement

The authors declare no conflicts of interest.

References

This article includes 32 references
  1. International Committee on Taxonomy of Viruses, ICTV Masters Species List 2022 MSL38 v3. [(accessed on 10 April 2024)]. Available online: https://ictv.global/msl.
  2. Horsington J.J., Gilkerson J.R., Hartley C.A.. Identification of mixed equine rhinitis B virus infections leading to further insight on the relationship between genotype, serotype and acid stability phenotype. Virus Res 2011;155:506–513.
    doi: 10.1016/j.virusres.2010.12.007pubmed: 21185890google scholar: lookup
  3. Horsington J.J., Lynch S.E., Gilkerson J.R., Studdert M.J., Hartley C.A.. Equine picornaviruses: Well known but poorly understood. Vet. Microbiol. 2013;167:78–85.
    doi: 10.1016/j.vetmic.2013.05.012pubmed: 23820049google scholar: lookup
  4. Diaz-Mendez A., Viel L., Hewson J., Doig P., Carman S., Chambers T., Tiwari A., Dewey C.. Surveillance of equine respiratory viruses in Ontario. Can. J. Vet. Res. 2010;74:271–278.
    pmc: PMC2949340pubmed: 21197227
  5. Dynon K., Black W.D., Ficorilli N., Hartley C.A., Studdert M.J.. Detection of viruses in nasal swab samples from horses with acute, febrile, respiratory disease using virus isolation, polymerase chain reaction and serology. Aust. Vet. J. 2007;85:46–50.
    doi: 10.1111/j.1751-0813.2006.00096.xpubmed: 17300454google scholar: lookup
  6. Mori A., De Benedictis P., Marciano S., Zecchin B., Zuin A., Zecchin B., Capua I., Cattoli G.. Development of a real-time duplex TaqMan-PCR for the detection of Equine rhinitis A and B viruses in clinical specimens. J. Virol. Methods 2009;155:175–181.
    doi: 10.1016/j.jviromet.2008.10.009pubmed: 19013197google scholar: lookup
  7. Quinlivan M., Maxwell G., Lyons P., Arkins S., Cullinane A.. Real-time RT-PCR for the detection and quantitative analysis of equine rhinitis viruses. Equine Vet. J. 2010;42:98–104.
    doi: 10.2746/042516409X479559pubmed: 20156243google scholar: lookup
  8. Lynch S.E., Gilkerson J.R., Symes S.J., Huang J.A., Hartley C.A.. Persistence and chronic urinary shedding of the aphthovirus equine rhinitis A virus. Comp. Immunol. Microbiol. Infect. Dis. 2013;36:95–103.
    doi: 10.1016/j.cimid.2012.10.003pubmed: 23183058google scholar: lookup
  9. Hartley C.A., Ficorilli N., Dynon K., Drummer H.E., Huang J.A., Studdert M.J.. Equine rhinitis A virus: Structural proteins and immune response. J. Gen. Virol. 2001;82:1725–1728.
    doi: 10.1099/0022-1317-82-7-1725pubmed: 11413384google scholar: lookup
  10. Woo P.C., Lau S.K., Choi G.K., Huang Y., Wernery R., Joseph S., Wong E.Y.M., Elizabeth S.K., Patteril N.A.G., Li T.. Equine rhinitis B viruses in horse fecal samples from the Middle East. Virol. J. 2016;13:94.
    doi: 10.1186/s12985-016-0547-xpmc: PMC4897857pubmed: 27267372google scholar: lookup
  11. Bażanów B., Frącka A., Jackulak N., Romuk E., Gębarowski T., Owczarek A., Stygar D.. Viral, serological, and antioxidant investigations of equine rhinitis a virus in serum and nasal swabs of commercially used horses in Poland. Biomed Res. Int. 2018;2018:8719281.
    doi: 10.1155/2018/8719281pmc: PMC5937552pubmed: 29850583google scholar: lookup
  12. Grądzki Z., Boguta L.. Seroprevalence of equine rhinitis B viruses in Poland. Med. Wet. 2009;65:119–123.
  13. Stasiak K., Dunowska M., Rola J.. Prevalence and sequence analysis of equid herpesviruses from the respiratory tract of Polish horses. Virol. J. 2018;15:106.
    doi: 10.1186/s12985-018-1018-3pmc: PMC6042439pubmed: 29996858google scholar: lookup
  14. Lu Z., Timoney P.J., White J., Balasuriya U.B.. Development of one-step TaqMan® real-time reverse transcription-PCR and conventional reverse transcription-PCR assays for the detection of equine rhinitis A and B viruses. BMC Vet. Res. 2012;8:120.
    doi: 10.1186/1746-6148-8-120pmc: PMC3542198pubmed: 22830930google scholar: lookup
  15. Black W.D., Hartley C.A., Ficorilli N.P., Studdert M.J.. Reverse transcriptase-polymerase chain reaction for the detection equine rhinitis B viruses and cell culture isolation of the virus. Arch. Virol. 2007;152:137–149.
    doi: 10.1007/s00705-006-0810-3pmc: PMC7086902pubmed: 16932985google scholar: lookup
  16. Tamura K., Stecher G., Kumar S.. MEGA11: Molecular Evolutionary Genetics Analysis Version 11. Mol. Biol. Evol. 2021;38:3022–3027.
    doi: 10.1093/molbev/msab120pmc: PMC8233496pubmed: 33892491google scholar: lookup
  17. Pusterla N., James K., Barnum S., Bain F., Barnett D.C., Chappell D., Gaughan E., Craig B., Schneider C., Vaala W.. Frequency of detection and prevalence factors associated with common respiratory pathogens in equids with acute onset of fever and/or respiratory signs (2008–2021). Pathogens 2022;11:759.
    doi: 10.3390/pathogens11070759pmc: PMC9317490pubmed: 35890002google scholar: lookup
  18. Mumford J.A., Thomson G.R.. Studies on picornaviruses isolated from the respiratory tract of horses. Equine Infectious Diseases IV. Proceedings of the Fourth International Conference on Equine Infectious Diseases 1978:419–429.
  19. Carman S., Rosendal S., Huber L., Gyles C., McKee S., Willoughby R.A., Dubovi E., Thorsen J., Lein D.. Infectious agents in acute respiratory disease in horses in Ontario. J. Vet. Diagn. Investig. 1997;9:17–23.
    doi: 10.1177/104063879700900104pubmed: 9087920google scholar: lookup
  20. Diaz-Mendez A., Hewson J., Shewen P., Viel L.. Characteristics of respiratory tract disease in horses inoculated with equine rhinitis A virus. Am. J. Vet. Res. 2014;75:169–178.
    doi: 10.2460/ajvr.75.2.169pubmed: 24471753google scholar: lookup
  21. Bernardino P., James K., Barnum S., Corbin R., Wademan C., Pusterla N.. What have we learned from 7 years of equine rhinitis B virus qPCR testing in nasal secretions from horses with respiratory signs. Vet. Rec. 2021;188:e26.
    doi: 10.1002/vetr.26pubmed: 33759193google scholar: lookup
  22. Schneider C., James K., Craig B.W., Chappel D.E., Vaala W., van Harreveld P.D., Wright C.A., Barnum S., Pusterla N.. Characterization of Equine Rhinitis B Virus Infection in Clinically Ill Horses in the United States during the Period 2012–2023. Pathogens 2023;12:1324.
    doi: 10.3390/pathogens12111324pmc: PMC10674924pubmed: 38003789google scholar: lookup
  23. Burrows R., Goodridge D. Observations of picornavirus, adenovirus, and equine herpesvirus infections in the Pirbright pony herd. In: Bryans J.T., Gerber H., editors. Proceedings of the 4th International Conference on Equine Infectious Diseases; Lyon, France. 24–27 September 1978; Lyon, France: Veterinary Publications; 1978. pp. 155–164.
  24. Pusterla N., Rice M., Henry T., Barnum S., James K.. Investigation of the shedding of selected respiratory pathogens in healthy horses presented for routine dental care. J. Vet. Dent. 2020;37:88–93.
    doi: 10.1177/0898756420949135pubmed: 32840159google scholar: lookup
  25. Wilcox A., Barnum S., Wademan C., Corbin R., Escobar E., Hodzic E., Schumacher S., Pusterla N.. Frequency of detection of respiratory pathogens in clinically healthy show horses following a multi-county outbreak of equine herpesvirus-1 myeloencephalopathy in California. Pathogens 2022;11:1161.
    doi: 10.3390/pathogens11101161pmc: PMC9612363pubmed: 36297218google scholar: lookup
  26. Pusterla N., Sandler-Burtness E., Barnum S., Hill L.A., Mendonsa E., Khan R., Portener D., Ridland H., Schumacher S.. Frequency of detection of respiratory pathogens in nasal secretions from healthy sport horses attending a spring show in California. J. Equine Vet. Sci. 2022;117:104089.
    doi: 10.1016/j.jevs.2022.104089pubmed: 35908600google scholar: lookup
  27. Smith F.L., Watson J.L., Spier S.J., Kilcoyne I., Mapes S., Sonder C., Pusterla N.. Frequency of shedding of respiratory pathogens in horses recently imported to the United States. J. Vet. Intern. Med. 2018;32:1436–1441.
    doi: 10.1111/jvim.15145pmc: PMC6060314pubmed: 29761571google scholar: lookup
  28. Stout A.E., Hofmar-Glennon H.G., André N.M., Goodman L.B., Anderson R.R., Mitchell P.K., Thompson B.S., Lejeune M., Whittaker G.R., Goodrich E.L.. Infectious disease surveillance of apparently healthy horses at a multi-day show using a novel nanoscale real-time PCR panel. J. Vet. Diagn. Investig. 2021;33:80–86.
    doi: 10.1177/1040638720972096pmc: PMC7758683pubmed: 33179576google scholar: lookup
  29. Kriegshäuser G., Deutz A., Kuechler E., Skern T., Lussy H., Nowotny N.. Prevalence of neutralizing antibodies to Equine rhinitis A and B virus in horses and man. Vet. Microbiol. 2005;106:293–296.
    doi: 10.1016/j.vetmic.2004.12.029pubmed: 15778036google scholar: lookup
  30. Black W.D., Wilcox R.S., Stevenson R.A., Hartley C.A., Ficorilli N.P., Gilkerson J.R., Studdert M.J.. Prevalence of serum neutralising antibody to equine rhinitis A virus (ERAV), equine rhinitis B virus 1 (ERBV1) and ERBV2. Vet. Microbiol. 2007;119:65–71.
    doi: 10.1016/j.vetmic.2006.08.031pubmed: 17046179google scholar: lookup
  31. Rossi T.M., Moore A., O’Sullivan T.L., Greer A.L.. Equine Rhinitis A Virus Infection at a Standardbred Training Facility: Incidence, Clinical Signs, and Risk Factors for Clinical Disease. Front. Vet. Sci. 2019;6:71.
    doi: 10.3389/fvets.2019.00071pmc: PMC6424864pubmed: 30918893google scholar: lookup
  32. Back H., Weld J., Walsh C., Cullinane A.. Equine Rhinitis A Virus Infection in Thoroughbred Racehorses—A Putative Role in Poor Performance?. Viruses 2019;11:963.
    doi: 10.3390/v11100963pmc: PMC6848918pubmed: 31635401google scholar: lookup

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