Progesterone Device Use Improves Ovum Pick-Up Efficiency in Acyclic Donors.
Abstract: The aim of this study was to evaluate follicular dynamics and ovum pick-up (OPU) efficacy in untreated mares or mares treated with an intravaginal progesterone (P4) device during seasonal anestrus (acyclic) and during the breeding season (cyclic). Six mares (mean age = 5 years), were recruited into an ovum pick-up scheme that was performed every 14 days with and without the P4 device, during the acyclic and cyclic phases. Aspirations amounted to seven procedures with or without the P4 device during each phase. Five ultrasound assessments were performed at each interval between the OPUs. Data on follicular number and diameter as well as the numbers of recovered and the percentage of recovered oocytes were also collected. The number of follicles from mares in the acyclic phase was higher (P < .005) regardless of the treatment. However, the follicular diameter was smaller for the P4 group (P < .005) from the 2nd to the 5th evaluation post-OPU procedure. The percentage of oocytes recovered during the acyclic phase was higher for mares treated with the P4 device (P < .005). The P4 device resulted in follicles with smaller diameters and facilitated OPU efficacy.
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Publication Date: 2023-09-29 PubMed ID: 37776919DOI: 10.1016/j.jevs.2023.104933Google Scholar: Lookup
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- Journal Article
Summary
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This study explored how the use of an intravaginal progesterone device in horses could enhance the efficiency of egg retrieval during both breeding and non-breeding seasons. The results showed that the device helped produce smaller follicles and increased the success rate of ovum pick-up procedures during the non-breeding season.
Study Design and Procedure
- The study aimed to compare follicular development and egg retrieval efficiency in horses during their breeding (cyclic) and non-breeding (acyclic) phases.
- For this experiment, six horses were selected and subjected to an ovum pick-up (OPU) process every two weeks — both with and without the use of a progesterone (P4) device. The procedures were conducted during both the cyclic and acyclic phases.
- The study consisted of seven aspirations that were performed both with and without the P4 device during each phase.
- In order to monitor the follicular dynamics, ultrasound assessments were performed five times during each interval between the OPU procedures.
Data Collection and Analysis
- The team collected data on the number and size of the follicles as well as the number of recovered oocytes and their percentage.
- This data was then analyzed for any significant differences between the untreated group and the group treated with the P4 device, in both the acyclic and cyclic phases.
Results and Conclusion
- Findings showed that the number of follicles was significantly higher for untreadted horses during the non-breeding phase, irrespective of whether the P4 device was used or not.
- Notably, though the follicular diameter was smaller for the P4 group during the non-breeding phase, the efficiency of OPU was greater.
- In conclusion, the use of a P4 device yielded follicles with smaller diameters and improved the success of the OPU procedures. Hence, it could potentially be a useful tool in increasing the efficiency of retrieving eggs from horses, especially during the non-breeding phase.
Cite This Article
APA
Fanhani GG, Cavalieri FLB, Andreazzi MA, da Silva CI, Andreazzi KC, Pereira LDC, Emanuelli IP, Dos Santos JMG, Moreski DAB, Colombo AHB, Morotti F, Seneda MM.
(2023).
Progesterone Device Use Improves Ovum Pick-Up Efficiency in Acyclic Donors.
J Equine Vet Sci, 131, 104933.
https://doi.org/10.1016/j.jevs.2023.104933 Publication
Researcher Affiliations
- Campus Maringá, Programa de Pós-Graduação em Tecnologias Limpas e Curso de Medicina Veterinária, Universidade Cesumar (UNICESUMAR), Maringá, Parana, Brazil.
- Campus Maringá, Programa de Pós-Graduação em Tecnologias Limpas e Curso de Medicina Veterinária, Universidade Cesumar (UNICESUMAR), Maringá, Parana, Brazil.
- Campus Maringá, Programa de Pós-Graduação em Tecnologias Limpas e Curso de Medicina Veterinária, Universidade Cesumar (UNICESUMAR), Maringá, Parana, Brazil.
- Programa de Pós-Graduação em Ciência de Alimentos, Universidade Estadual de Maringá (UEM), Maringá, Parana, Brazil.
- Campus Maringá, Programa de Pós-Graduação em Tecnologias Limpas e Curso de Medicina Veterinária, Universidade Cesumar (UNICESUMAR), Maringá, Parana, Brazil.
- Campus Maringá, Programa de Pós-Graduação em Tecnologias Limpas e Curso de Medicina Veterinária, Universidade Cesumar (UNICESUMAR), Maringá, Parana, Brazil.
- Campus Maringá, Programa de Pós-Graduação em Tecnologias Limpas e Curso de Medicina Veterinária, Universidade Cesumar (UNICESUMAR), Maringá, Parana, Brazil.
- Campus Maringá, Programa de Pós-Graduação em Tecnologias Limpas e Curso de Medicina Veterinária, Universidade Cesumar (UNICESUMAR), Maringá, Parana, Brazil.
- Campus Maringá, Programa de Pós-Graduação em Tecnologias Limpas e Curso de Medicina Veterinária, Universidade Cesumar (UNICESUMAR), Maringá, Parana, Brazil.
- Campus Maringá, Programa de Pós-Graduação em Tecnologias Limpas e Curso de Medicina Veterinária, Universidade Cesumar (UNICESUMAR), Maringá, Parana, Brazil.
- Laboratório de Biotecnologia da Reprodução Animal, Universidade Estadual de Londrina (UEL), Londrina, Parana, Brazil.
- Laboratório de Biotecnologia da Reprodução Animal, Universidade Estadual de Londrina (UEL), Londrina, Parana, Brazil. Electronic address: marcelo.seneda@uel.br.
MeSH Terms
- Animals
- Female
- Horses
- Progesterone
- Ovarian Follicle / diagnostic imaging
- Oocytes
- Anestrus
Conflict of Interest Statement
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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