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American journal of veterinary research2023; 85(1); doi: 10.2460/ajvr.23.07.0150

Radiological features of arterial channels in the equine third phalanx measured using a novel customized software represent changes of laminitis.

Abstract: To identify and measure radiolucencies at the solear margin of the distal phalanx in radiographs of healthy and laminitic hooves. Methods: Clinical records and dorsoproximal-palmarodistal radiographs of equine forelimbs with radiological diagnoses of either laminitis (n = 40, L) or navicular syndrome (n = 40, NS). Methods: Outlines of the radiolucent structures at the solar margin were drawn in ImageJ, and a customized novel plugin "Arteries Analyzer/ImageJ" was used for measurements. The diverging radiolucencies outside the terminal arc of the distal phalanx were differentiated as arterial channels (open at the solear margin) and ellipses (closed at the solear margin). Comparisons between L and NS, between distal phalanges with and without ellipses, and of arterial channels and ellipses in areas were compared using Wilcoxon and the Mann-Whitney U tests, respectively. The reliability and repeatability of the method were tested using Friedman's test. Results: Fewer arterial channels but more ellipses were identified in L than in NS. In phalanges with ellipses (n = 47), the number of ellipses and the number of arterial channels were negatively correlated (PCC -0.181, P = .224). The number of ellipses correlated positively with the severity of laminitis (PCC 0.495, P < .001; n = 80) and with the degree of rotation of the distal phalanx (PCC 0.392, P < .001; n = 80). Conclusions: The software tool successfully measured arterial channels and ellipses outlined by the evaluators. Results indicate that healthy arteries develop into pathological ellipses in laminitic feet. This may be used to complement the interpretation of radiographs and support clinical decision-making.
Publication Date: 2023-11-01 PubMed ID: 37903449DOI: 10.2460/ajvr.23.07.0150Google Scholar: Lookup
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  • Journal Article

Summary

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The research focuses on measuring changes in the structure of horse hooves due to a disease called laminitis, using a new customized software. According to the study, these changes are visible in x-ray images of the hooves and could serve an important role in diagnosing and treating the disease.

Research Methodology

The study used two groups of horse forelimbs (40 with laminitis, 40 with navicular syndrome). X-ray images of these hooves were scrutinised for changes in their structure due to the disease conditions. A software called ImageJ and a custom plugin “Arteries Analyzer/ImageJ” were used to outline and measure different radiolucent structures at the margin of the solar (the bottom part of the hoof). These measurements aimed to identify changes in the hoof due to laminitis.

The researchers particularly focused on the following areas in their analysis:

  • Radiolucencies outside the terminal arc of the distal phalanx.
  • The presence of arterial channels and ellipses at the solear margin.

Statistical tests including Wilcoxon and the Mann-Whitney U tests were used to differentiate the conditions and compare the measurements obtained.

Results and Conclusions

The measurements from the software showed fewer arterial channels and more ellipses in hooves with laminitis when compared to those with navicular syndrome. There was a negative correlation found between the number of ellipses and the number of arterial channels in hooves that contained ellipses.

Interestingly, the study found a positive correlation between the number of ellipses and both the severity of laminitis and the degree of rotation of the distal phalanx. These findings indicate that arterial channels in healthy hooves might turn into pathological ellipses in laminitic feet.

In conclusion, the software tool was successful in measuring the arterial channels and ellipses as outlined by the researchers. Thus, these measurements might provide valuable insights for diagnosing and treating laminitis, making the software a helpful tool in clinical decision-making.

Cite This Article

APA
Reiser V, Reiser A, Licka TF. (2023). Radiological features of arterial channels in the equine third phalanx measured using a novel customized software represent changes of laminitis. Am J Vet Res, 85(1). https://doi.org/10.2460/ajvr.23.07.0150

Publication

ISSN: 1943-5681
NlmUniqueID: 0375011
Country: United States
Language: English
Volume: 85
Issue: 1

Researcher Affiliations

Reiser, Valentina
  • Clinic for Horses, Department for Horses and Small Animals, Vienna University of Veterinary Medicine, Vienna, Austria.
Reiser, Andreas
  • Clinic for Horses, Department for Horses and Small Animals, Vienna University of Veterinary Medicine, Vienna, Austria.
Licka, Theresia F
  • Clinic for Horses, Department for Horses and Small Animals, Vienna University of Veterinary Medicine, Vienna, Austria.
  • Department of Veterinary Clinical Studies, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Roslin Midlothian, Scotland.

MeSH Terms

  • Horses
  • Animals
  • Reproducibility of Results
  • Radiography
  • Forelimb / diagnostic imaging
  • Forelimb / pathology
  • Bone and Bones / pathology
  • Arteries
  • Horse Diseases / diagnostic imaging
  • Horse Diseases / pathology
  • Foot Diseases / diagnostic imaging
  • Foot Diseases / veterinary
  • Foot Diseases / pathology