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Home / Equine Research Bank / BMC Vet Res / Rate of manual leukocyte differentials in dog, cat and horse...
BMC veterinary research2014; 10; 125; doi: 10.1186/1746-6148-10-125

Rate of manual leukocyte differentials in dog, cat and horse blood samples using ADVIA 120 cytograms.

Abstract: Modern automated haematology instruments are capable of performing leukocyte differentials faster, cheaper and with a higher precision than the traditional 100-cell manual differential count. Thus, in human laboratories, criteria are defined for performing a manual review of the blood smear resulting in a marked reduction of manual differential counts. While common in human laboratories, this approach to reducing the number of manual differentials in veterinary laboratories is still not commonly performed. Thus, our aim was to determine the rate and causes of manual leukocyte differentials in a university clinical pathology laboratory using the automated laser-based haematology analyser ADVIA 120. Overall, 14,953 complete blood cell counts from dogs, cats and horses were reviewed. Manual leukocyte differentials were requested if abnormal ADVIA peroxidase and baso cytograms were detected (i.e. suspicion of left shift or atypical lymphocytes/blasts, inappropriate separation of cell populations). Results: In 21% of canine, 32% of feline and 20% of equine samples, a manual differential was requested. Indistinct separation of the cell population was present in 10% to 15% of the cases. Depending on the species, atypical lymphocytes were suspected in 2% to 12%, left shift in 13% to 25% and suspicion of blasts was present in less than 0.4% of the cases. Conclusions: The obtained results are comparable to those published for human medicine and the rate of manual differentiation could be markedly reduced in veterinary laboratories if microscopic examination was used as a validation procedure rather than as a reflexive substitute for automated differentiation.
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Publication Date: 2014-06-05 PubMed ID: 24903909PubMed Central: PMC4053280DOI: 10.1186/1746-6148-10-125Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses how modern automated haematology instruments efficiently perform leukocyte differentials on animal blood samples, potentially minimizing the need for manual review. The research aims to determine the rate and reasons for manual leukocyte differentials in a veterinary clinical pathology laboratory.

Study Methodology and Process

  • The study utilized an automated laser-based haematology analyser known as ADVIA 120 to review complete blood cell counts (CBCs) from dogs, cats, and horses. The total number of samples reviewed was 14,953.
  • Manual leukocyte differentials were carried out on samples where abnormal ADVIA peroxidase and baso cytograms were detected. These occur when there is suspicion of a left shift, atypical lymphocytes/blasts or improper separation of cell populations.

Research Findings

  • Canine, feline, and equine samples required a manual differential in 21%, 32%, and 20% of the cases respectively.
  • There was an indistinct separation in the cell population in around 10% to 15% of the samples.
  • Depending on the species, suspicion of atypical lymphocytes was in the range of 2% to 12%, left shift suspicion in 13% to 25%, and suspicion for the presence of blasts was less than 0.4%.
  • The data gathered from this study were similar to those documented in human medicine literature.

Conclusions and Implications

  • The study suggests that there could be a significant reduction in manual differentiation if veterinary laboratories used microscopic examination only as a validation process, not a reflexive substitute for automated differentiation.
  • The implication is that employing automated haematology instruments for leukocyte differentials can result in cost and time efficiency in veterinary laboratories just as it has in human laboratories.

Cite This Article

APA
Stirn M, Moritz A, Bauer N. (2014). Rate of manual leukocyte differentials in dog, cat and horse blood samples using ADVIA 120 cytograms. BMC Vet Res, 10, 125. https://doi.org/10.1186/1746-6148-10-125

Publication

BMC veterinary research
ISSN: 1746-6148
NlmUniqueID: 101249759
Country: England
Language: English
Volume: 10
Pages: 125

Researcher Affiliations

Stirn, Martina
    Moritz, Andreas
      Bauer, Natali
      • Department of Veterinary Clinical Sciences, Clinical Pathology and Clinical Pathophysiology, Justus-Liebig University Giessen, Frankfurterstr, 126, Giessen 35392, Germany. natali.bauer@vetmed.uni-giessen.de.

      MeSH Terms

      • Animals
      • Automation
      • Cats / blood
      • Dogs / blood
      • Horses / blood
      • Leukocyte Count / methods
      • Leukocyte Count / veterinary
      • Leukocytes / classification
      • Lymphocyte Subsets
      • Reproducibility of Results
      • Sensitivity and Specificity
      • Species Specificity

      References

      This article includes 23 references
      1. Moritz A. Der Einsatz lasergestützter Multiparameter-Hämatologiesysteme in der Veterinärmedizin. Wetzlar, Germany: Pandora Verlag Wetzlar; 2000.
      2. Moritz A, Fickenscher Y, Meyer K, Failing K, Weiss DJ. Canine and feline hematology reference values for the ADVIA 120 hematology system.. Vet Clin Pathol 2004;33(1):32-8.
        doi: 10.1111/j.1939-165X.2004.tb00347.xpubmed: 15048625google scholar: lookup
      3. Lilliehöök I, Tvedten H. Validation of the Sysmex XT-2000iV hematology system for dogs, cats, and horses. II. Differential leukocyte counts.. Vet Clin Pathol 2009 Jun;38(2):175-82.
        doi: 10.1111/j.1939-165X.2009.00126.xpubmed: 19392762google scholar: lookup
      4. Lilliehöök I, Tvedten H. Validation of the Sysmex XT-2000iV hematology system for dogs, cats, and horses. I. Erythrocytes, platelets, and total leukocyte counts.. Vet Clin Pathol 2009 Jun;38(2):163-74.
        doi: 10.1111/j.1939-165X.2009.00125.xpubmed: 19392763google scholar: lookup
      5. Neuerer FF. Evaluation des vollautomatischen Hämatologiegerätes CELL-DYN 3500 im klinischen Einsatz bei Hund und Katze. Munich, Germany: Dissertation München; 2005.
      6. Bauer N, Nakagawa J, Dunker C, Failing K, Moritz A. Evaluation of the automated hematology analyzer Sysmex XT-2000iV™ compared to the ADVIA® 2120 for its use in dogs, cats, and horses: Part I--precision, linearity, and accuracy of complete blood cell count.. J Vet Diagn Invest 2011 Nov;23(6):1168-80.
        doi: 10.1177/1040638711425572pubmed: 22362798google scholar: lookup
      7. Bauer N, Nakagawa J, Dunker C, Failing K, Moritz A. Evaluation of the automated hematology analyzer Sysmex XT-2000iV ™ compared to the ADVIA ® 2120 for its use in dogs, cats, and horses. Part II: Accuracy of leukocyte differential and reticulocyte count, impact of anticoagulant and sample aging.. J Vet Diagn Invest 2012 Jan;24(1):74-89.
        doi: 10.1177/1040638711436243pubmed: 22362937google scholar: lookup
      8. Mathers RA, Evans GO, Bleby J, Tornow T. Evaluation of the Sysmex XT-2000iV haematology analyser for rat, dog and mouse whole blood samples. Comp Clin Pathol 2008;17:137–144.
        doi: 10.1007/s00580-008-0734-2google scholar: lookup
      9. Goldmann F, Bauer N, Moritz A. Evaluation of the IDEXX ProCyte Dx analyzer for dogs and cats compared to the Siemens ADVIA 2120 and manual differential. Comp Clin Pathol 2012;2303:283–296.
      10. Goldmann F, Bauer N, Moritz A. Comparison of the ProCyte Dx analyzer with the ADVIA 2120 and the manual differential for validation of equine and bovine hemograms. Comp Clin Pathol 2012;2205:855–868.
      11. Novis DA, Walsh M, Wilkinson D, St Louis M, Ben-Ezra J. Laboratory productivity and the rate of manual peripheral blood smear review: a College of American Pathologists Q-Probes study of 95,141 complete blood count determinations performed in 263 institutions.. Arch Pathol Lab Med 2006 May;130(5):596-601.
        pubmed: 16683868doi: 10.5858/2006-130-596-lpatrogoogle scholar: lookup
      12. Rabizadeh E, Pickholtz I, Barak M, Froom P. Historical data decrease complete blood count reflex blood smear review rates without missing patients with acute leukaemia.. J Clin Pathol 2013 Aug;66(8):692-4.
        doi: 10.1136/jclinpath-2012-201423pubmed: 23505267google scholar: lookup
      13. Lantis KL, Harris RJ, Davis G, Renner N, Finn WG. Elimination of instrument-driven reflex manual differential leukocyte counts. Optimization of manual blood smear review criteria in a high-volume automated hematology laboratory.. Am J Clin Pathol 2003 May;119(5):656-62.
        doi: 10.1309/VH1KMV8WB7GB7R14pubmed: 12760283google scholar: lookup
      14. Tvedten HW, Haines C. Canine automated differential leukocyte count: study using a hematology analyzer system.. Vet Clin Pathol 1994;23(3):90-96.
        doi: 10.1111/j.1939-165X.1994.tb00686.xpubmed: 12666023google scholar: lookup
      15. Tvedten HW, Korcal D. Automated differential leukocyte count in horses, cattle, and cats using the Technicon H-1E hematology system.. Vet Clin Pathol 1996;25(1):14-22.
        doi: 10.1111/j.1939-165X.1996.tb00960.xpubmed: 12660982google scholar: lookup
      16. Barnes PW, McFadden SL, Machin SJ, Simson E. The international consensus group for hematology review: suggested criteria for action following automated CBC and WBC differential analysis.. Lab Hematol 2005;11(2):83-90.
        doi: 10.1532/LH96.05019pubmed: 16024331google scholar: lookup
      17. Bain BJ. Diagnosis from the blood smear.. N Engl J Med 2005 Aug 4;353(5):498-507.
        doi: 10.1056/NEJMra043442pubmed: 16079373google scholar: lookup
      18. Bayer Health Care LLC. ADVIA 120 Operator's Guide. 2004.
      19. Harris N, Kunicka J, Kratz A. The ADVIA 2120 hematology system: flow cytometry-based analysis of blood and body fluids in the routine hematology laboratory.. Lab Hematol 2005;11(1):47-61.
        doi: 10.1532/LH96.04075pubmed: 15790553google scholar: lookup
      20. Moritz A, Hoffmann C. [Platelet count in the cat].. Tierarztl Prax Ausg K Kleintiere Heimtiere 1997 Nov;25(6):695-700.
        pubmed: 9459839
      21. RUEMKE CL. Laboratory aids. Variability of results in differential cell counts on blood smears.. Triangle 1960 Jan;4:154-8.
        pubmed: 14440049
      22. Clark P, Mogg TD, Tvedten HW, Korcal D. Artifactual changes in equine blood following storage, detected using the Advia 120 hematology analyzer.. Vet Clin Pathol 2002;31(2):90-4.
        doi: 10.1111/j.1939-165X.2002.tb00286.xpubmed: 12040491google scholar: lookup
      23. Furlanello T, Tasca S, Caldin M, Carli E, Patron C, Tranquillo M, Lubas G, Solano-Gallego L. Artifactual changes in canine blood following storage, detected using the ADVIA 120 hematology analyzer.. Vet Clin Pathol 2006 Mar;35(1):42-6.
        doi: 10.1111/j.1939-165X.2006.tb00087.xpubmed: 16511790google scholar: lookup

      Citations

      This article has been cited 2 times.
      1. Oikonomidis IL, Milne E, Piccinelli C. Differential white blood cell counts in rabbits: a comparison of the Advia 2120 and a manual method.. J Vet Diagn Invest 2021 Jul;33(4):670-676.
        doi: 10.1177/10406387211007877pubmed: 33834920google scholar: lookup
      2. Dondi F, Vasylyeva K, Serafini F, Gruarin M, Troìa R, Giunti M, Agnoli C. Heinz body-related interference with leukocyte and erythrocyte variables obtained by an automated hematology analyzer in cats.. J Vet Diagn Invest 2019 Sep;31(5):704-713.
        doi: 10.1177/1040638719863088pubmed: 31291839google scholar: lookup
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