Recombinant vesicular stomatitis (Indiana) virus expressing New Jersey and Indiana glycoproteins induces neutralizing antibodies to each serotype in swine, a natural host.
Abstract: Vesicular stomatitis virus (VSV) is the most common cause of vesicular disease outbreaks in livestock throughout the Western Hemisphere. Two major serotypes, Indiana and New Jersey, cause epidemic disease in pigs, cattle, and horses. We generated recombinant viruses derived from the Indiana serotype genome that were engineered to contain and express: (1) a single copy of the glycoprotein gene from the Indiana serotype (VSIV-GI); (2) a single copy of the glycoprotein gene from the New Jersey serotype (VSIV-GNJ); or (3) two copies of the glycoprotein gene, one from each of the two major VSV serotypes (VSIV-GNJGI) [Martinez I, Rodriguez LL, Jimenez C, Pauszek SJ, Wertz GW. Vesicular stomatitis virus glycoprotein is a determinant of pathogenesis in swine, a natural host. J Virol 2003;77(14):8039-47]. These recombinant viruses and a highly pathogenic New Jersey field isolate (VSNJV), from which the GNJ gene was derived, were inoculated into swine, a natural host, and the induction of neutralizing antibodies to both serotypes was analyzed. The neutralizing antibody response induced by VSIV-GI, VSIV-GNJ and VSNJV was serotype-specific, according to the glycoprotein expressed. VSIV-GNJGI expressed both glycoproteins stably through multiple rounds of replication in swine and induced neutralizing antibodies against both VSV serotypes, with a dominance of the Indiana serotype in the serological response. Pigs immunized with VSIV-GI or VSIV-GNJ were protected against homologous high dose virus challenge. Pigs inoculated with VSIV-GNJGI were protected against challenge with VSIV-GI but three of four animals developed lesions after challenge with the highly pathogenic New Jersey field isolate.
Publication Date: 2004-09-15 PubMed ID: 15364454DOI: 10.1016/j.vaccine.2004.03.065Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Research Support
- U.S. Gov't
- P.H.S.
Summary
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The study is focused on the creation of recombinant vesicular stomatitis viruses that can induce immune responses to two major disease causing serotypes in livestock, with a goal of developing vaccines that provide protection against both strains.
Introduction
- Vesicular stomatitis virus (VSV) is a significant cause of disease in livestock throughout the Western Hemisphere, affecting pigs, cattle, and horses. Its two main serotypes, Indiana (VSVI) and New Jersey (VSVNJ), wreak havoc by causing severe outbreaks.
- This research focuses on genetically engineering the VSV viruses, derived from the VSVI genome, to express glycoproteins from both VSVI and VSVNJ serotypes, with the aim of provoking an immune response to both serotypes.
Methods and Results
- Three types of recombinant viruses (VSIV-GI, VSIV-GNJ, and VSIV-GNJGI) were generated for this study.
- The VSIV-GI and VSIV-GNJ were engineered to contain a single copy of the glycoprotein gene from either VSVI or VSVNJ respectively. VSIV-GNJGI, however, was engineered to express glycoproteins from both serotypes.
- In addition, a highly pathogenic field isolate from the VSNJV was included in the study.
- These modified viruses were then inoculated into swine and the resultant immune response observed.
- The VSIV-GI, VSIV-GNJ, and VSNJV induced serotype-specific neutralizing antibodies in the swine, reinforcing the link between the glycoprotein expressed and the specificity of the immune response.
- VISV-GNJGI demonstrated the ability to stably express both serotype glycoproteins in pigs and induced neutralizing antibodies against both viruses, although the VSVI glycoprotein had greater dominance in the serological response.
Protection against Homologous Challenge
- It was also revealed that swine immunized with VSIV-GI and VSIV-GNJ were able to endure challenges from high doses of the homologous virus, highlighting their potential as vaccines.
- Those inoculated with VSIV-GNJGI were safeguarded against VSVI challenges, but showed limited protective superiority over the highly pathogenic field isolate from VSVNJ.
Conclusion
- This study presented a promising approach to managing VSV, particularly by the investigation of genetic modifications of the virus to induce immunity against both major VSV serotypes. However, additional work is needed to increase the efficiency of the induced immunity.
Cite This Article
APA
Martinez I, Barrera JC, Rodriguez LL, Wertz GW.
(2004).
Recombinant vesicular stomatitis (Indiana) virus expressing New Jersey and Indiana glycoproteins induces neutralizing antibodies to each serotype in swine, a natural host.
Vaccine, 22(29-30), 4035-4043.
https://doi.org/10.1016/j.vaccine.2004.03.065 Publication
Researcher Affiliations
- Department of Microbiology, University of Alabama School of Medicine, Birmingham, AL 35294, USA. lrodriguez@piadc.ars.usda.gov
MeSH Terms
- Animals
- Antibodies, Viral / blood
- Glycoproteins / genetics
- Glycoproteins / immunology
- Neutralization Tests
- Rhabdoviridae Infections / prevention & control
- Rhabdoviridae Infections / veterinary
- Swine / immunology
- Swine Diseases / prevention & control
- Vaccines, Synthetic / administration & dosage
- Vaccines, Synthetic / genetics
- Vaccines, Synthetic / immunology
- Vesicular stomatitis Indiana virus / classification
- Vesicular stomatitis Indiana virus / genetics
- Vesicular stomatitis Indiana virus / immunology
- Vesiculovirus
- Viral Proteins / genetics
- Viral Proteins / immunology
- Viral Vaccines / administration & dosage
- Viral Vaccines / genetics
- Viral Vaccines / immunology
Grant Funding
- R37AI12464 / NIAID NIH HHS
Citations
This article has been cited 7 times.- Lütge M, Pikor NB, Ludewig B. Differentiation and activation of fibroblastic reticular cells. Immunol Rev 2021 Jul;302(1):32-46.
- Xue X, Yu Z, Jin H, Liang L, Li J, Li X, Wang Y, Cui S, Li G. Recombinant adenovirus expressing vesicular stomatitis virus G proteins induce both humoral and cell-mediated immune responses in mice and goats. BMC Vet Res 2021 Jan 18;17(1):36.
- Velazquez-Salinas L, Pauszek SJ, Holinka LG, Gladue DP, Rekant SI, Bishop EA, Stenfeldt C, Verdugo-Rodriguez A, Borca MV, Arzt J, Rodriguez LL. A Single Amino Acid Substitution in the Matrix Protein (M51R) of Vesicular Stomatitis New Jersey Virus Impairs Replication in Cultured Porcine Macrophages and Results in Significant Attenuation in Pigs. Front Microbiol 2020;11:1123.
- Velazquez-Salinas L, Naik S, Pauszek SJ, Peng KW, Russell SJ, Rodriguez LL. Oncolytic Recombinant Vesicular Stomatitis Virus (VSV) Is Nonpathogenic and Nontransmissible in Pigs, a Natural Host of VSV. Hum Gene Ther Clin Dev 2017 Jun;28(2):108-115.
- Fang X, Zhang S, Sun X, Li J, Sun T. Evaluation of attenuated VSVs with mutated M or/and G proteins as vaccine vectors. Vaccine 2012 Feb 8;30(7):1313-21.
- Lee HS, Heo EJ, Jeoung HY, Ko HR, Kweon CH, Youn HJ, Ko YJ. Enzyme-linked immunosorbent assay using glycoprotein and monoclonal antibody for detecting antibodies to vesicular stomatitis virus serotype New Jersey. Clin Vaccine Immunol 2009 May;16(5):667-71.
- Russell SJ, Peng KW. Viruses as anticancer drugs. Trends Pharmacol Sci 2007 Jul;28(7):326-33.
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