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Home / Equine Research Bank / Viruses / Seroprevalence and Risk Factors of Crimean-Congo Hemorrhagic...
Viruses2025; 17(3); 387; doi: 10.3390/v17030387

Seroprevalence and Risk Factors of Crimean-Congo Hemorrhagic Fever Exposure in Wild and Domestic Animals in Benin.

Abstract: Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne zoonotic viral disease prevalent in Africa. While infection is asymptomatic in animals, it can cause severe illness with hemorrhagic manifestations and high mortality rates in humans. This study aimed to determine the seroprevalence and potential risk factors of CCHF in wild (rodents, birds) and domestic (cattle, horses) animals in Benin. A cross-sectional study was carried out from 2022 to 2024 with the assistance of cattle breeders, hunters, farmers and bushmeat sellers in 15 districts found in three agroecological zones in the country. A total of 366 serum samples were analyzed, comprising 254 collected from wild animals and 112 from domestic animals. Among the wild animals tested, 1.18% (95% CI: 0.31-3.70; n = 3) were seropositive for antibodies against CCHF virus (CCHFV). The seroprevalence rates were 3.7% (95% CI: 0.19-20.89) in squirrels, 5.88% (95% CI: 0.31-30.76) in hares and 1.19% (95% CI: 0.06-7.38) in giant rats. In domestic animals, anti-CCHFV antibodies were detected in 38 of the 112 samples, resulting in an overall seroprevalence of 33.93% (95% CI: 25.42-43.56). Specifically, antibodies were identified in 34 out of 81 cattle (41.98%, 95% CI: 31.26-53.46) and 4 out of 24 horses (16.67%, 95% CI: 5.48-38.19). No positive samples were reported in pigeons. This study provides the first seroprevalence data on CCHF in wild and domestic animals in Benin. It highlights the risk and epidemiological dynamics of the disease and underscores the need for further investigations into tick vectors and human populations.
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Publication Date: 2025-03-08 PubMed ID: 40143315PubMed Central: PMC11946281DOI: 10.3390/v17030387Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study aim was to establish the prevalence and risk factors for the spread of Crimean-Congo hemorrhagic fever (CCHF) in wild and domestic animals in Benin. They found 3 wild animals of the 254 tested and 38 domestic animals of the 112 tested had antibodies for CCHF. Their findings present first such data from Benin and call for further investigation especially towards tick vectors in human populations.

Objective of the Research

  • This research was conducted with the aim to understand the seroprevalence, or the measure of the presence of specific antibodies in serums, and potential risk factors of Crimean-Congo Hemorrhagic Fever (CCHF) amongst wild and domestic animals in Benin. CCHF is a tick-borne viral disease that primarily affects animals but can cause severe illness in humans.

Research Conducted and Analysis

  • The researchers carried out a cross-sectional study from 2022 to 2024 across three agroecological zones and 15 districts in Benin. They were assisted by stakeholders including cattle breeders, hunters, farmers, and bushmeat sellers.
  • A total of 366 serum samples were analyzed – 254 from wild animals (such as rodents, birds) and 112 from domesticated animals (cattle and horses).
  • The study reveals that 1.18% of wild animals and 33.93% of domestic animals tested were found to have antibodies against the CCHF virus (CCHFV), indicating exposure to the virus.
  • In terms of specific animal species wise prevalence, antibodies were identified in 3.7% squirrels, 5.88% hares, and 1.19% giant rats tested. Among domestic animals, 41.98% of the cattle and 16.67% of the horses were found to carry anti-CCHFV antibodies. However, none of the tested pigeons were found with positive samples.

Significance of the Findings

  • This research provides the first seroprevalence data on CCHF in both wild and domestic animals in Benin.
  • The findings of the study help comprehend the risk and epidemiological dynamics of the disease in the region, thus, providing valuable information for future preventive measures.
  • The high seroprevalence in domestic animals and presence in wild animals point toward the need for further investigation particularly concerning tick vectors, which are the transmission agents of the virus, and potential exposure in human populations.

Cite This Article

APA
Yessinou RE, Farougou S, Olopade JO, Oluwayelu DO, Happi A, Happi C, Groschup M. (2025). Seroprevalence and Risk Factors of Crimean-Congo Hemorrhagic Fever Exposure in Wild and Domestic Animals in Benin. Viruses, 17(3), 387. https://doi.org/10.3390/v17030387

Publication

Viruses
ISSN: 1999-4915
NlmUniqueID: 101509722
Country: Switzerland
Language: English
Volume: 17
Issue: 3
PII: 387

Researcher Affiliations

Yessinou, Roland Eric
  • Communicable Diseases Research Unit, Department of Production and Animal Health, University of Abomey-Calavi, Abomey Calavi 229, Benin.
Farougou, Souaïbou
  • Communicable Diseases Research Unit, Department of Production and Animal Health, University of Abomey-Calavi, Abomey Calavi 229, Benin.
Olopade, James Olukayode
  • Department of Veterinary Anatomy, Faculty of Veterinary Medicine, University of Ibadan, Ibadan 200284, Nigeria.
  • Humboldt Research Hub for Zoonotic Arboviral Diseases, Faculty of Veterinary Medicine, University of Ibadan, Ibadan 200284, Nigeria.
Oluwayelu, Daniel Oladimeji
  • Humboldt Research Hub for Zoonotic Arboviral Diseases, Faculty of Veterinary Medicine, University of Ibadan, Ibadan 200284, Nigeria.
  • Department of Veterinary Microbiology, Faculty of Veterinary Medicine, University of Ibadan, Ibadan 200284, Nigeria.
Happi, Anise
  • African Center of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer's University, Oshogbo 232102, Nigeria.
Happi, Christian
  • African Center of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer's University, Oshogbo 232102, Nigeria.
Groschup, Martin
  • Friedrich-Loeffler-Institut, Institute of Novel and Emerging Infectious Diseases, Südufer 10, 17493 Greifswald, Insel Riems, Germany.

MeSH Terms

  • Animals
  • Hemorrhagic Fever, Crimean / epidemiology
  • Hemorrhagic Fever, Crimean / veterinary
  • Hemorrhagic Fever, Crimean / virology
  • Seroepidemiologic Studies
  • Benin / epidemiology
  • Animals, Domestic / virology
  • Risk Factors
  • Animals, Wild / virology
  • Cross-Sectional Studies
  • Hemorrhagic Fever Virus, Crimean-Congo / immunology
  • Cattle
  • Antibodies, Viral / blood
  • Humans
  • Horses
  • Zoonoses / epidemiology
  • Zoonoses / virology
  • Birds / virology
  • Male
  • Ticks / virology
  • Female

Grant Funding

  • None / Alexander von Humboldt Foundation

Conflict of Interest Statement

The authors declare no conflict of interest.

References

This article includes 45 references
  1. Chala B, Hamde F. Emerging and re-emerging vector-borne infectious diseases and the challenges for control: A review. Front. Public Health 2021;9:715759.
    doi: 10.3389/fpubh.2021.715759pmc: PMC8524040pubmed: 34676194google scholar: lookup
  2. Hanagandi P.B., Gonçalves F.G., do Amaral L.L.F., Chong J.J.R., Chankowsky J., Torres C.I., del Carpio R., Vakharia R.J., Torres C., Jaggi S. Multidisciplinary Approach to Tropical and Subtropical Infectious Diseases: Imaging with Pathologic Correlation. Neurographics 2015;5:258–278.
    doi: 10.3174/ng.6150133google scholar: lookup
  3. Temur A.I., Kuhn J.H., Pecor D.B., Apanaskevich D.A., Keshtkar-Jahromi M. Epidemiology of Crimean-Congo hemorrhagic fever (CCHF) in Africa—Underestimated for decades. Am. J. Trop. Med. Hyg. 2021;104:1978.
    doi: 10.4269/ajtmh.20-1413pmc: PMC8176481pubmed: 33900999google scholar: lookup
  4. Belobo J.T.E., Kenmoe S., Kengne-Nde C., Emoh C.P.D., Bowo-Ngandji A., Tchatchouang S., Sowe Wobessi J.N., Mbongue Mikangue C.A., Tazokong H.R., Kingue Bebey S.R. Worldwide epidemiology of Crimean-Congo Hemorrhagic Fever Virus in humans, ticks and other animal species, a systematic review and meta-analysis. PLoS Negl. Trop. Dis. 2021;15:e0009299.
    doi: 10.1371/journal.pntd.0009299pmc: PMC8096040pubmed: 33886556google scholar: lookup
  5. Bouchard C., Dibernardo A., Koffi J., Wood H., Leighton P.A., Lindsay L.R. Climate change and infectious diseases: The challenges: N Increased risk of tick-borne diseases with climate and environmental changes. Can. Commun. Dis. Rep. 2019;45:83.
    doi: 10.14745/ccdr.v45i04a02pmc: PMC6587693pubmed: 31285697google scholar: lookup
  6. Spengler J.R., Estrada-Peña A. Host preferences support the prominent role of Hyalomma ticks in the ecology of Crimean-Congo hemorrhagic fever. PLoS Negl. Trop. Dis. 2018;12:e0006248.
    doi: 10.1371/journal.pntd.0006248pmc: PMC5821391pubmed: 29420542google scholar: lookup
  7. Nasirian H. Crimean-Congo hemorrhagic fever (CCHF) seroprevalence: A systematic review and meta-analysis. Acta Trop. 2019;196:102–120.
    doi: 10.1016/j.actatropica.2019.05.019pubmed: 31108083google scholar: lookup
  8. White N.J., Watson J.A., Uyoga S., Williams T.N., Maitland K.M. Substantial misdiagnosis of severe malaria in African children. Lancet 2022;400:807.
    doi: 10.1016/S0140-6736(22)01600-2pubmed: 36088944google scholar: lookup
  9. Vonesch N., Binazzi A., Bonafede M., Melis P., Ruggieri A., Iavicoli S., Tomao P. Emerging zoonotic viral infections of occupational health importance. Pathog. Dis. 2019;77:ftz018.
    doi: 10.1093/femspd/ftz018pmc: PMC7108535pubmed: 30916772google scholar: lookup
  10. Spengler J.R., Bergeron É., Rollin P.E. Seroepidemiological studies of Crimean-Congo hemorrhagic fever virus in domestic and wild animals. PLoS Negl. Trop. Dis. 2016;10:e0004210.
    doi: 10.1371/journal.pntd.0004210pmc: PMC4704823pubmed: 26741652google scholar: lookup
  11. Bernard C., Holzmuller P., Bah M.T., Bastien M., Combes B., Jori F., Grosbois V., Vial L. Systematic review on Crimean–Congo hemorrhagic fever enzootic cycle and factors favoring virus transmission: Special focus on France, an apparently free-disease area in Europe. Front. Vet. Sci. 2022;9:932304.
    doi: 10.3389/fvets.2022.932304pmc: PMC9343853pubmed: 35928117google scholar: lookup
  12. Corniaux C., Thébaud B., Powell A., Apolloni A., Touré I. Cross-Border Livestock Mobility: Challenges for Wet Africa. .
  13. Estrada-Peña A., Gray J.S., Kahl O., Lane R.S., Nijhoff A.M. Research on the ecology of ticks and tick-borne pathogens—Methodological principles and caveats. Front. Cell. Infect. Microbiol. 2013;3:29.
    doi: 10.3389/fcimb.2013.00029pmc: PMC3737478pubmed: 23964348google scholar: lookup
  14. Spengler J.R., Estrada-Peña A., Garrison A.R., Schmaljohn C., Spiropoulou C.F., Bergeron É., Bente D.A. A chronological review of experimental infection studies of the role of wild animals and livestock in the maintenance and transmission of Crimean-Congo hemorrhagic fever virus. Antivir. Res. 2016;135:31–47.
    doi: 10.1016/j.antiviral.2016.09.013pmc: PMC5102700pubmed: 27713073google scholar: lookup
  15. Mukhaye E., Akoko J.M., Nyamota R., Mwatondo A., Muturi M., Nthiwa D., Kirwa L.J., Bargul J.L., Abkallo H.M., Bett B. Exposure patterns and the risk factors of Crimean Congo hemorrhagic fever virus amongst humans, livestock and selected wild animals at the human/livestock/wildlife interface in Isiolo County, upper eastern Kenya. PLoS Negl. Trop. Dis. 2024;18:e0012083.
    doi: 10.1371/journal.pntd.0012083pmc: PMC11423962pubmed: 39269988google scholar: lookup
  16. Muzammil K., Rayyani S., Abbas Sahib A., Gholizadeh O., Naji Sameer H., Jwad Kazem T., Badran Mohammed H., Ghafouri Kalajahi H., Zainul R., Yasamineh S. Recent Advances in Crimean-Congo Hemorrhagic Fever Virus Detection, Treatment, and Vaccination: Overview of Current Status and Challenges. Biol. Proced. Online 2024;26:20.
    doi: 10.1186/s12575-024-00244-3pmc: PMC11201903pubmed: 38926669google scholar: lookup
  17. Daodu O.B., Eisenbarth A., Schulz A., Hartlaub J., Olopade J.O., Oluwayelu D.O., Groschup M.H. Molecular detection of dugbe orthonairovirus in cattle and their infesting ticks (Amblyomma and Rhipicephalus (Boophilus)) in Nigeria. PLoS Negl. Trop. Dis. 2021;15:e0009905.
    doi: 10.1371/journal.pntd.0009905pmc: PMC8598060pubmed: 34788303google scholar: lookup
  18. Hartlaub J., Daodu O.B., Sadeghi B., Keller M., Olopade J., Oluwayelu D., Groschup M.H. Cross-Reaction or Co-Infection? Serological Discrimination of Antibodies Directed against Dugbe and Crimean-Congo Hemorrhagic Fever Orthonairovirus in Nigerian Cattle. Viruses 2021;13:1398.
    doi: 10.3390/v13071398pmc: PMC8310240pubmed: 34372604google scholar: lookup
  19. Matthews J., Secka A., McVey D.S., Dodd K.A., Faburay B. Serological Prevalence of Crimean–Congo Hemorrhagic Fever Virus Infection in Small Ruminants and Cattle in The Gambia. Pathogens 2023;12:749.
    doi: 10.3390/pathogens12060749pmc: PMC10302318pubmed: 37375439google scholar: lookup
  20. Oumorou M., Lejoly J. Écologie, flore et végétation de l’inselberg Sobakpérou (nord-Bénin). Acta Bot. Gall. 2003;150:65–84.
    doi: 10.1080/12538078.2003.10515987google scholar: lookup
  21. Vanvanhossou S.F.U., Dossa L.H., König S. Sustainable management of animal genetic resources to improve low-input livestock production: Insights into local Beninese cattle populations. Sustainability 2021;13:9874.
    doi: 10.3390/s጗9874google scholar: lookup
  22. Yassegoungbe F.P., Oloukoi D., Aoudji A.K.N., Schlecht E., Dossa L.H. Insights into the diversity of cow milk production systems on the fringes of coastal cities in West Africa: A case study from Benin. Front. Sustain. Food Syst. 2022;6:1001497.
    doi: 10.3389/fsufs.2022.1001497google scholar: lookup
  23. Houessou S.O., Dossa L.H., Diogo R.V.C., Ahozonlin M.C., Dahouda M., Schlecht E. Confronting pastoralists’ knowledge of cattle breeds raised in the extensive production systems of Benin with multivariate analyses of morphological traits. PLoS ONE 2019;14:e0222756.
    doi: 10.1371/journal.pone.0222756pmc: PMC6762103pubmed: 31557214google scholar: lookup
  24. Ahozonlin M.C., Gbangboche A.B., Dossa L.H. Current Knowledge on the Lagune Cattle Breed in Benin: A State of the Art Review. Ruminants 2022;2:271–281.
    doi: 10.3390/ruminants2020018google scholar: lookup
  25. Scherf B.D., Pilling D. The Second Report on the State of the World’s Animal Genetic Resources for Food and Agriculture. .
  26. Fanelli A., Buonavoglia D. Risk of Crimean Congo haemorrhagic fever virus (CCHFV) introduction and spread in CCHF-free countries in southern and Western Europe: A semi-quantitative risk assessment. One Health 2021;13:100290.
    doi: 10.1016/j.onehlt.2021.100290pmc: PMC8283130pubmed: 34307823google scholar: lookup
  27. Kuehnert P.A., Stefan C.P., Badger C.V., Ricks K.M. Crimean-Congo Hemorrhagic Fever Virus (CCHFV): A Silent but Widespread Threat. Curr. Trop. Med. Rep. 2021;8:141–147.
    doi: 10.1007/s40475-021-00235-4pmc: PMC7959879pubmed: 33747715google scholar: lookup
  28. Schüpbach G., Silva L.C., Buzzell-Hatav A. Surveillance plan proposal for early detection of zoonotic pathogens in ruminants. EFSA Support. Publ. 2023;20:7887E.
    doi: 10.2903/sp.efsa.2023.EN-7887google scholar: lookup
  29. Bespyatova L.A., Bugmyrin S.V., Kutenkov S.A., Nikonorova I.A. Abundance of Ixodid Ticks (Acari: Ixodidae) on Small Mammals in Forest Biotopes of the Middle Taiga Subzone of Karelia. Entomol. Rev. 2021;101:273–281.
    doi: 10.1134/S0013873821020147google scholar: lookup
  30. Estrada-Peña A., de la Fuente J. The ecology of ticks and epidemiology of tick-borne viral diseases. Antivir. Res. 2014;108:104–128.
    doi: 10.1016/j.antiviral.2014.05.016pubmed: 24925264google scholar: lookup
  31. Shah T., Li Q., Wang B., Baloch Z., Xia X. Geographical distribution and pathogenesis of ticks and tick-borne viral diseases. Front. Microbiol. 2023;14:1185829.
    doi: 10.3389/fmicb.2023.1185829pmc: PMC10244671pubmed: 37293222google scholar: lookup
  32. Palomar A.M., Portillo A., Santibáñez P., Mazuelas D., Arizaga J., Crespo A., Gutiérrez Ó., Cuadrado J.F., Oteo J.A. Crimean-Congo hemorrhagic fever virus in ticks from migratory birds, Morocco. Emerg. Infect. Dis. 2013;19:260.
    doi: 10.3201/eid1902.121193pmc: PMC3559059pubmed: 23347801google scholar: lookup
  33. Chumakov M.P. Translation Editor’s Introduction. .
    doi: 10.4182/KGVP3013.9-3.125google scholar: lookup
  34. Assogbadjo A.E., Codjia J.T.C., Sinsin B., Ekue M.R.M., Mensah G.A. Importance of rodents as a human food source in Benin. Belg. J. Zool. 2005;135:11–15.
  35. Yessinou R.E., Adoligbe C., Akpo Y., Adinci J., Youssao Abdou Karim I., Farougou S. Sensitivity of Different Cattle Breeds to the Infestation of Cattle Ticks Amblyomma variegatum, Rhipicephalus microplus, and Hyalomma spp. on the Natural Pastures of Opkara Farm, Benin. J. Parasitol. Res. 2018;2018:2570940.
    doi: 10.1155/2018/2570940pmc: PMC5889886pubmed: 29770229google scholar: lookup
  36. Kumsa B., Tamrat H., Tadesse G., Aklilu N., Cassini R. Prevalence and species composition of ixodid ticks infesting horses in three agroecologies in central Oromia, Ethiopia. Trop. Anim. Health Prod. 2012;44:119–124.
    doi: 10.1007/s11250-011-9897-ypubmed: 21656133google scholar: lookup
  37. Payne V.K., Mbafor F.L., Wabo Pone J., Tchoumboué J. Preliminary study of ectoparasites of horses in the western highlands of Cameroon. Vet. Med. Sci. 2017;3:63–70.
    doi: 10.1002/vms3.56pmc: PMC5488173pubmed: 28713574google scholar: lookup
  38. Mangombi J.B., Roqueplo C., Sambou M., Dahmani M., Mediannikov O., Comtet L., Davoust B. Seroprevalence of Crimean-Congo hemorrhagic fever in domesticated animals in Northwestern Senegal. Vector-Borne Zoonotic Dis. 2020;20:797–799.
    doi: 10.1089/vbz.2019.2592pubmed: 32429789google scholar: lookup
  39. Oluwayelu D., Afrough B., Adebiyi A., Varghese A., Eun-Sil P., Fukushi S., Yoshikawa T., Saijo M., Neumann E., Morikawa S.. Prevalence of Antibodies to Crimean-Congo Hemorrhagic Fever Virus in Ruminants, Nigeria, 2015. Emerg. Infect. Dis. 2020;26:744–747.
    doi: 10.3201/eid2604.190354pmc: PMC7101109pubmed: 32186489google scholar: lookup
  40. Dzikwi-Emennaa A.A., Meseko C., Emennaa P., Adeyinka A.J., Adamu A.M., Adegboye O.A. Detection of Crimean-Congo Hemorrhagic Fever Virus Antibodies in Cattle in Plateau State, Nigeria. Viruses 2022;14:2618.
    doi: 10.3390/v14122618pmc: PMC9787510pubmed: 36560622google scholar: lookup
  41. Maiga O., Sas M.A., Rosenke K., Kamissoko B., Mertens M., Sogoba N., Traore A., Sangare M., Niang M., Schwan T.G. Serosurvey of Crimean–Congo hemorrhagic fever virus in cattle, Mali, West Africa. Am. J. Trop. Med. Hyg. 2017;96:1341.
    doi: 10.4269/ajtmh.16-0818pmc: PMC5462568pubmed: 28719259google scholar: lookup
  42. Schulz A., Barry Y., Stoek F., Ba A., Schulz J., Haki M.L., Sas M.A., Doumbia B.A., Kirkland P., Bah M.Y. Crimean-Congo hemorrhagic fever virus antibody prevalence in Mauritanian livestock (cattle, goats, sheep and camels) is stratified by the animal’s age. PLoS Negl. Trop. Dis. 2021;15:e0009228.
    doi: 10.1371/journal.pntd.0009228pmc: PMC8081336pubmed: 33844691google scholar: lookup
  43. Valery A.E., Aimée D.-K.C., Maxime D.K., Clémence K.K.A.R.M., Mireille D. Serosurvey of Crimean-Congo Haemorrhagic Fever Virus (CCHFV) in Cattle in Livestock Areas of Côte d’Ivoire, West Africa. Microbiol. Res. J. Int. 2021;31:11–18.
    doi: 10.9734/mrji/2021/v31i130287google scholar: lookup
  44. Phonera M.C., Simuunza M.C., Kainga H., Ndebe J., Chembensofu M., Chatanga E., Kanyanda S., Changula K., Muleya W., Mubemba B. Seroprevalence and risk factors of Crimean-Congo hemorrhagic fever in cattle of smallholder farmers in Central Malawi. Pathogens 2021;10:1613.
    doi: 10.3390/pathogens10121613pmc: PMC8709441pubmed: 34959568google scholar: lookup
  45. Karanam S.K., Nagvishnu K., Uppala P.K., Edhi S., Varri S.R. Crimean-Congo hemorrhagic fever: Pathogenesis, transmission and public health challenges. World J. Virol. 2025;14:100003.
    doi: 10.5501/wjv.v14.i1.100003pmc: PMC11612873pubmed: 40134837google scholar: lookup

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