Seroprevalence of influenza D virus in bulls in Argentina.
Abstract: Influenza D virus (IDV) is considered a new agent involved in bovine respiratory disease (BRD). Based on seroprevalence studies or isolation from clinical samples, this virus has been detected on several continents and in several animal species, including cattle, pigs, camel, horses, and goats. We used an indirect in-house ELISA to detect anti-IDV antibodies in 165 serum samples from bulls on 116 farms in the province of La Pampa, Argentina. Eighty-five of 116 (73%) farms had at least 1 positive animal, and 112 of 165 (68%) of the analyzed samples were positive. There were no significant differences in the proportion of seropositive samples depending on the geographic region in which the samples were taken. Our results suggest that IDV infection is endemic in La Pampa; the clinical importance of IDV in Argentina remains to be investigated.
Publication Date: 2020-06-18 PubMed ID: 32552516PubMed Central: PMC7438650DOI: 10.1177/1040638720934056Google Scholar: Lookup
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- Journal Article
Summary
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This study reveals that a significant majority of bulls in La Pampa, Argentina, carry antibodies for the Influenza D virus (IDV), indicating the virus is endemic in the area.
Objective and Methodology
- The research aimed to evaluate the presence and prevalence of the Influenza D virus, a potential infectious agent in bovine respiratory disease, in cattle in the province of La Pampa, Argentina.
- The team utilized an in-house indirect ELISA (Enzyme-Linked Immunosorbent Assay) for verifying the presence of anti-IDV antibodies. This technique is widely used to detect antigens or antibodies in a sample.
- They collected 165 serum samples from bulls across 116 farms in different parts of the province for their analysis.
Results and Interpretation
- The analysis showed that 112 out of 165 samples (68%) were positive for anti-IDV antibodies. This indicates that these bulls had been exposed to the virus at some point in their lives.
- On a farm level, it was found that 85 out of 116 farms (73%) had at least one positive animal.
- Interestingly, a significant disparity in the proportion of seropositive samples based on the geographical region was not found. This suggests that the virus’s spread is relatively uniform across the province.
Implications and Further Research
- The high seroprevalence of IDV implies that the virus is endemic in the La Pampa region. However, the study does not determine if or how it contributes to bovine respiratory disease or other health problems in the cattle population.
- Although the study highlights the widespread nature of IDV in La Pampa, more research is necessary to understand the virus’s clinical importance in cattle in Argentina and its potential effect on public health or the agriculture industry.
- Further studies should also investigate whether other animal species that interact with cattle in the region are also potential carriers of the virus, as IDV has been found in various animals such as pigs, camels, horses, and goats on other continents.
Cite This Article
APA
Alvarez IJ, Fort M, Pasucci J, Moreno F, Gimenez H, Näslund K, Hägglund S, Zohari S, Valarcher JF.
(2020).
Seroprevalence of influenza D virus in bulls in Argentina.
J Vet Diagn Invest, 32(4), 585-588.
https://doi.org/10.1177/1040638720934056 Publication
Researcher Affiliations
- Laboratorio Alvarez, Bahía Blanca, Buenos Aires, Argentina (Alvarez).
- Instituto Nacional de Tecnología Agropecuaria, Anguil, La Pampa, Argentina (Fort, Gimenez).
- Centro de Investigacion Veterinaria Tandil, Buenos Aires, Argentina (Pasucci).
- Instituto Nacional de Tecnología Agropecuaria, Tandil, Buenos Aires, Argentina (Moreno).
- Swedish University of Agricultural Sciences, Host Pathogen Interaction Group, Department of Clinical Sciences, Uppsala, Sweden (Näslund, Hägglund, Valarcher, Zohari).
- Department of Microbiology, National Veterinary Institute, Uppsala, Sweden (Näslund, Zohari).
- Laboratorio Alvarez, Bahía Blanca, Buenos Aires, Argentina (Alvarez).
- Instituto Nacional de Tecnología Agropecuaria, Anguil, La Pampa, Argentina (Fort, Gimenez).
- Centro de Investigacion Veterinaria Tandil, Buenos Aires, Argentina (Pasucci).
- Instituto Nacional de Tecnología Agropecuaria, Tandil, Buenos Aires, Argentina (Moreno).
- Swedish University of Agricultural Sciences, Host Pathogen Interaction Group, Department of Clinical Sciences, Uppsala, Sweden (Näslund, Hägglund, Valarcher, Zohari).
- Department of Microbiology, National Veterinary Institute, Uppsala, Sweden (Näslund, Zohari).
- Laboratorio Alvarez, Bahía Blanca, Buenos Aires, Argentina (Alvarez).
- Instituto Nacional de Tecnología Agropecuaria, Anguil, La Pampa, Argentina (Fort, Gimenez).
- Centro de Investigacion Veterinaria Tandil, Buenos Aires, Argentina (Pasucci).
- Instituto Nacional de Tecnología Agropecuaria, Tandil, Buenos Aires, Argentina (Moreno).
- Swedish University of Agricultural Sciences, Host Pathogen Interaction Group, Department of Clinical Sciences, Uppsala, Sweden (Näslund, Hägglund, Valarcher, Zohari).
- Department of Microbiology, National Veterinary Institute, Uppsala, Sweden (Näslund, Zohari).
- Laboratorio Alvarez, Bahía Blanca, Buenos Aires, Argentina (Alvarez).
- Instituto Nacional de Tecnología Agropecuaria, Anguil, La Pampa, Argentina (Fort, Gimenez).
- Centro de Investigacion Veterinaria Tandil, Buenos Aires, Argentina (Pasucci).
- Instituto Nacional de Tecnología Agropecuaria, Tandil, Buenos Aires, Argentina (Moreno).
- Swedish University of Agricultural Sciences, Host Pathogen Interaction Group, Department of Clinical Sciences, Uppsala, Sweden (Näslund, Hägglund, Valarcher, Zohari).
- Department of Microbiology, National Veterinary Institute, Uppsala, Sweden (Näslund, Zohari).
- Laboratorio Alvarez, Bahía Blanca, Buenos Aires, Argentina (Alvarez).
- Instituto Nacional de Tecnología Agropecuaria, Anguil, La Pampa, Argentina (Fort, Gimenez).
- Centro de Investigacion Veterinaria Tandil, Buenos Aires, Argentina (Pasucci).
- Instituto Nacional de Tecnología Agropecuaria, Tandil, Buenos Aires, Argentina (Moreno).
- Swedish University of Agricultural Sciences, Host Pathogen Interaction Group, Department of Clinical Sciences, Uppsala, Sweden (Näslund, Hägglund, Valarcher, Zohari).
- Department of Microbiology, National Veterinary Institute, Uppsala, Sweden (Näslund, Zohari).
- Laboratorio Alvarez, Bahía Blanca, Buenos Aires, Argentina (Alvarez).
- Instituto Nacional de Tecnología Agropecuaria, Anguil, La Pampa, Argentina (Fort, Gimenez).
- Centro de Investigacion Veterinaria Tandil, Buenos Aires, Argentina (Pasucci).
- Instituto Nacional de Tecnología Agropecuaria, Tandil, Buenos Aires, Argentina (Moreno).
- Swedish University of Agricultural Sciences, Host Pathogen Interaction Group, Department of Clinical Sciences, Uppsala, Sweden (Näslund, Hägglund, Valarcher, Zohari).
- Department of Microbiology, National Veterinary Institute, Uppsala, Sweden (Näslund, Zohari).
- Laboratorio Alvarez, Bahía Blanca, Buenos Aires, Argentina (Alvarez).
- Instituto Nacional de Tecnología Agropecuaria, Anguil, La Pampa, Argentina (Fort, Gimenez).
- Centro de Investigacion Veterinaria Tandil, Buenos Aires, Argentina (Pasucci).
- Instituto Nacional de Tecnología Agropecuaria, Tandil, Buenos Aires, Argentina (Moreno).
- Swedish University of Agricultural Sciences, Host Pathogen Interaction Group, Department of Clinical Sciences, Uppsala, Sweden (Näslund, Hägglund, Valarcher, Zohari).
- Department of Microbiology, National Veterinary Institute, Uppsala, Sweden (Näslund, Zohari).
- Laboratorio Alvarez, Bahía Blanca, Buenos Aires, Argentina (Alvarez).
- Instituto Nacional de Tecnología Agropecuaria, Anguil, La Pampa, Argentina (Fort, Gimenez).
- Centro de Investigacion Veterinaria Tandil, Buenos Aires, Argentina (Pasucci).
- Instituto Nacional de Tecnología Agropecuaria, Tandil, Buenos Aires, Argentina (Moreno).
- Swedish University of Agricultural Sciences, Host Pathogen Interaction Group, Department of Clinical Sciences, Uppsala, Sweden (Näslund, Hägglund, Valarcher, Zohari).
- Department of Microbiology, National Veterinary Institute, Uppsala, Sweden (Näslund, Zohari).
- Laboratorio Alvarez, Bahía Blanca, Buenos Aires, Argentina (Alvarez).
- Instituto Nacional de Tecnología Agropecuaria, Anguil, La Pampa, Argentina (Fort, Gimenez).
- Centro de Investigacion Veterinaria Tandil, Buenos Aires, Argentina (Pasucci).
- Instituto Nacional de Tecnología Agropecuaria, Tandil, Buenos Aires, Argentina (Moreno).
- Swedish University of Agricultural Sciences, Host Pathogen Interaction Group, Department of Clinical Sciences, Uppsala, Sweden (Näslund, Hägglund, Valarcher, Zohari).
- Department of Microbiology, National Veterinary Institute, Uppsala, Sweden (Näslund, Zohari).
MeSH Terms
- Animals
- Antibodies, Viral / blood
- Argentina / epidemiology
- Cattle
- Cattle Diseases / epidemiology
- Cattle Diseases / virology
- Enzyme-Linked Immunosorbent Assay / veterinary
- Male
- Orthomyxoviridae Infections / epidemiology
- Orthomyxoviridae Infections / veterinary
- Orthomyxoviridae Infections / virology
- Prevalence
- Seroepidemiologic Studies
- Thogotovirus / isolation & purification
Conflict of Interest Statement
The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
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Citations
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