Sinus lift with autologous bone alone or in addition to equine bone: an immunohistochemical study in man.
Abstract: The aim of the present study was to perform a comparative histological and immunohistochemical evaluation of microvessel density, vascular endothelial growth factor, and nitric oxide synthase (NOS) expression in sinus augmentation using autologous bone alone or in combination with equine bone. Methods: Sixteen patients underwent sinus augmentation with autologous bone and a 50:50 mixture of autologous and equine bone. Control cores were harvested from preexisting nonaugmented bone under the sinus floor. The specimens were processed for immunohistochemistry. Results: The greatest values of microvessel density were found in the sites grafted with autologous bone with significant differences between control versus autologous group (P < 0.01) and control versus autologous + equine group (P < 0.01). The higher and lower intensities of vascular endothelial growth factor and NOS3 expression were prevalent in the sites grafted with autologous bone with significant differences with the controls (P < 0.05). No significant differences (P > 0.05) were found in the NOS1 expression among the groups. Conclusions: The results obtained showed that the mixture of autologous and equine bone was biocompatible, and its use was associated with new blood vessels ingrowth during healing, which has been found to be extremely important for bone formation.
Publication Date: 2011-09-13 PubMed ID: 21909027DOI: 10.1097/ID.0b013e3182310b3dGoogle Scholar: Lookup
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- Comparative Study
- Journal Article
Summary
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The research is conducted to compare the effects of using autologous (sourced from the patient) bone alone or combined with equine bone for sinus augmentation, given that they impact microvessel density, vascular endothelial growth factor, and nitric oxide synthase expression values.
Research Methodology
- Sixteen patients were selected and both the autologous bone alone and a 50:50 mixture of autologous and equine bone were used for sinus augmentation procedure.
- Control cores (or untreated sinus samples) were also gathered from nonaugmented bone under the sinus floor.
- These specimens were then processed for immunohistochemistry, a testing method that uses antibodies to check for specific antigens in tissue samples.
Findings
- Higher microvessel density, Vascular Endothelial Growth Factor (VEGF) – a key driver in blood vessel formation, and Nitric Oxide Synthase 3 (NOS3) – responsible for endothelial nitric oxide production, were found in sites grafted with autologous bone.
- These parameters showed notable difference against the control values for autologous & autologous + equine groups (P values less than 0.01).
- Notably the NOS1 expression, different from NOS3 and prominent in nerve cells, didn’t show any significant difference among all groups.
Conclusions
- The use of a combined autologous and equine bone approach was found to be biocompatible and associated with the growth of new blood vessels during the healing process.
- This influx of blood vessels is critical for successful bone formation. Hence the admixture could be used as a safe and effective option for sinus augmentation techniques.
Cite This Article
APA
Artese L, Piattelli A, Di Stefano DA, Piccirilli M, Pagnutti S, D'Alimonte E, Perrotti V.
(2011).
Sinus lift with autologous bone alone or in addition to equine bone: an immunohistochemical study in man.
Implant Dent, 20(5), 383-388.
https://doi.org/10.1097/ID.0b013e3182310b3d Publication
Researcher Affiliations
- Dental School, University of Chieti-Pescara, Chieti, Italy.
MeSH Terms
- Adult
- Aged
- Animals
- Biocompatible Materials / therapeutic use
- Bone Transplantation / methods
- Bone Transplantation / pathology
- Collagen
- Female
- Horses
- Humans
- Image Processing, Computer-Assisted
- Immunohistochemistry
- Jaw, Edentulous, Partially / rehabilitation
- Jaw, Edentulous, Partially / surgery
- Male
- Maxilla / surgery
- Membranes, Artificial
- Microscopy, Video
- Microvessels / pathology
- Middle Aged
- Nitric Oxide Synthase Type I / analysis
- Nitric Oxide Synthase Type III / analysis
- Osteogenesis / physiology
- Platelet Endothelial Cell Adhesion Molecule-1 / analysis
- Sinus Floor Augmentation / methods
- Surgical Flaps
- Transplantation, Autologous
- Transplantation, Heterologous
- Vascular Endothelial Growth Factor A / analysis
Citations
This article has been cited 15 times.- Di Stefano DA, Orlando F, Ottobelli M, Fiori D, Garagiola U. A comparison between anorganic bone and collagen-preserving bone xenografts for alveolar ridge preservation: systematic review and future perspectives. Maxillofac Plast Reconstr Surg 2022 Jul 12;44(1):24.
- Tumedei M, Mijiritsky E, Mourão CF, Piattelli A, Degidi M, Mangano C, Iezzi G. Histological and Biological Response to Different Types of Biomaterials: A Narrative Single Research Center Experience over Three Decades. Int J Environ Res Public Health 2022 Jun 28;19(13).
- Piolanti N, Del Chiaro A, Matassi F, Nistri L, Graceffa A, Marcucci M. Bone integration in acetabular revision hip arthroplasty using equine-derived bone grafts: a retrospective study. Eur J Orthop Surg Traumatol 2020 May;30(4):575-581.
- Di Stefano DA, Greco G, Gherlone E. A Preshaped Titanium Mesh for Guided Bone Regeneration with an Equine-Derived Bone Graft in a Posterior Mandibular Bone Defect: A Case Report. Dent J (Basel) 2019 Aug 1;7(3).
- Di Stefano DA, Zaniol T, Cinci L, Pieri L. Chemical, Clinical and Histomorphometric Comparison between Equine Bone Manufactured through Enzymatic Antigen-Elimination and Bovine Bone Made Non-Antigenic Using a High-Temperature Process in Post-Extractive Socket Grafting. A Comparative Retrospective Clinical Study. Dent J (Basel) 2019 Jul 1;7(3).
- Lo Giudice R, Rizzo G, Centofanti A, Favaloro A, Rizzo D, Cervino G, Squeri R, Costa BG, La Fauci V, Lo Giudice G. Steam Sterilization of Equine Bone Block: Morphological and Collagen Analysis. Biomed Res Int 2018;2018:9853765.
- La Monaca G, Iezzi G, Cristalli MP, Pranno N, Sfasciotti GL, Vozza I. Comparative Histological and Histomorphometric Results of Six Biomaterials Used in Two-Stage Maxillary Sinus Augmentation Model after 6-Month Healing. Biomed Res Int 2018;2018:9430989.
- Mattioli B, Iacoviello P, Aldiano C, Verrina G. Subcranial Le Fort III Advancement with Equine-Derived Bone Grafts to Correct Syndromic Midfacial Hypoplasia: A Case Report. J Maxillofac Oral Surg 2018 Sep;17(3):296-300.
- Lorenzo FDR. Treating a Recalcitrant Non-union of the Radius Using Autogenous Bone, Equine Bone Paste, Equine Demineralized Bone Matrix, Platelet Rich Plasma, and Bone Marrow Aspirate. A Case Report. J Orthop Case Rep 2017 Nov-Dec;7(6):31-35.
- Di Stefano DA, Greco GB, Riboli F. Guided Bone Regeneration of an Atrophic Mandible with a Heterologous Bone Block. Craniomaxillofac Trauma Reconstr 2016 Mar;9(1):88-93.
- Annibali S, Iezzi G, Sfasciotti GL, Cristalli MP, Vozza I, Mangano C, La Monaca G, Polimeni A. Histological and Histomorphometric Human Results of HA-Beta-TCP 30/70 Compared to Three Different Biomaterials in Maxillary Sinus Augmentation at 6 Months: A Preliminary Report. Biomed Res Int 2015;2015:156850.
- Papavasileiou DA, Leventis M, Agrogiannis G, Kalyvas D. Effect of Isolating the Periosteum With a Resorbable Barrier Membrane on Neoangiogenesis in Guided Bone Regeneration: An Experimental Study. Cureus 2025 Mar;17(3):e81069.
- Maiotti M, Massoni C. Arthroscopic Xenograft With Cerclage Fixation: A Method for Glenoid Bone Loss Reconstruction With Cerclage Fixation Using a Specific Posterior Guide. Arthrosc Tech 2023 Oct;12(10):e1657-e1664.
- Yamaguchi K, Munakata M, Sato D, Kataoka Y, Kawamata R. The Effectiveness and Practicality of a Novel Barrier Membrane for the Open Window in Maxillary Sinus Augmentation with a Lateral Approach, with Risk Indicators for Bone Graft Displacement and Bone Height Decrease: A Prospective Study in Humans. Bioengineering (Basel) 2023 Sep 22;10(10).
- Maiotti M, Massoni C, Di Pietto F, Romano M, Guastafierro A, Della Rotonda G, Russo R. Arthroscopic Subscapularis Augmentation With Xenograft Glenoid Bone Block in Patients With Recurrent Anterior Shoulder Instability. Arthrosc Sports Med Rehabil 2023 Jun;5(3):e809-e816.
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