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In vitro1984; 20(3 Pt 1); 198-204; doi: 10.1007/BF02618188

Spermidine cytotoxicity in vitro: effect of serum and oxygen tension.

Abstract: Plasma amine oxidase activities (benzylamine oxidase and spermine oxidase) were determined in the sera of a number of species of various ages. Benzylamine oxidase (BZO) activity, measured spectrophotometrically, was present in bovine, equine, and ovine species examined. Generally its activity in serum increased with the age of the animal. Spermine oxidase activity (SPO) was estimated by a bioassay of in vitro toxicity and did not necessarily correlate with BZO. Cytotoxicity in the presence of spermidine was found only in the sera of the ruminant species examined. Serum activity tended to rise with animal age; however, great variability was found in perinatal bovine sera. The 50% lethal dose (LD50) of spermidine in the presence of 5% serum and 4 X 10(4) NS1 cells/ml was in the micromolar range. Aminoguanidine, a known inhibitor of SPO, could prevent the cytotoxic effects of exogenously added spermidine in vitro. In contrast, raising the ambient oxygen tension in the incubation environment to 95% lowered the LD50 dose of spermidine required for cytotoxicity. The results suggest that a cell line of hematogenous origin is susceptible to the cytotoxic effects of the products of oxidative deamination of spermidine by SPO, an enzyme present in perinatal bovine sera, and that these cytotoxic effects are potentiated in the presence of an oxygen-enriched environment in vitro.
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Publication Date: 1984-03-01 PubMed ID: 6715006DOI: 10.1007/BF02618188Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • U.S. Gov't
  • P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article focuses on the in vitro cytotoxicity of Spermidine and how it is affected by serum and oxygen tension. The study unearths the key role of plasma amine oxidase activities, and their increase with age, in the resultant cytotoxic effects, while also highlighting that higher oxygen levels can intensify these effects.

Discussion on Plasma Amine Oxidase Activities

  • The researchers measured the activities of plasma amine oxidase activities, specifically benzylamine oxidase (BZO) and spermine oxidase (SPO), in the sera of various species at different ages.
  • The BZO activity was observed in bovine, horse, and sheep, with its activity generally increasing as the animal aged. This activity was measured spectrophotometrically.
  • SPO activity, however, did not necessarily correspond with BZO activity.

Spermidine Cytotoxicity

  • The cytotoxic effects of spermidine were uniquely observed in the sera of ruminants. This implies that the cytotoxicity of spermidine varied among different species.
  • Serum activity tended to rise as the animal aged, much like BZO; however, there were significant deviations in perinatal bovine sera.
  • The lethal dose of spermidine, under the conditions of 5% serum and 4 X 10(4) NS1 cells/ml, was within the micromolar range. This shows that the cytotoxic effects of spermidine can be profound at a relatively low concentration.

Impact of Aminoguanidine and Oxygen Tension

  • The cytotoxic effects of spermidine could be thwarted by aminoguanidine, an inhibitor of SPO. This suggests a relationship between SPO and spermidine cytotoxicity.
  • Increasing the ambient oxygen tension to 95% significantly reduced the required lethal dose of spermidine. This finding indicated that higher oxygen levels could potentiate the cytotoxic effects of spermidine.

Summary

  • The study concludes that a specific cell line of hematogenous origin can suffer cytotoxic effects from oxidative deamination products of spermidine, an occurrence mediated by SPO.
  • The enzyme SPO, found in perinatal bovine sera, was crucial in this process. The presence of an oxygen-rich environment in vitro significantly enhanced these cytotoxic effects.

Cite This Article

APA
Hegre OD, Marshall S, Hickey GE. (1984). Spermidine cytotoxicity in vitro: effect of serum and oxygen tension. In Vitro, 20(3 Pt 1), 198-204. https://doi.org/10.1007/BF02618188

Publication

In vitro
ISSN: 0073-5655
NlmUniqueID: 0063733
Country: United States
Language: English
Volume: 20
Issue: 3 Pt 1
Pages: 198-204

Researcher Affiliations

Hegre, O D
    Marshall, S
      Hickey, G E

        MeSH Terms

        • Aging
        • Animals
        • Benzylamine Oxidase / blood
        • Cattle / blood
        • Cell Line
        • Cell Survival / drug effects
        • Chickens / blood
        • Guanidines / pharmacology
        • Horses / blood
        • Kinetics
        • Lethal Dose 50
        • Mice
        • Monoamine Oxidase / blood
        • Oxidoreductases Acting on CH-NH Group Donors / blood
        • Oxygen / pharmacology
        • Plasmacytoma
        • Sheep / blood
        • Spermidine / toxicity

        Grant Funding

        • AM 19899 / NIADDK NIH HHS
        • AM 32237 / NIADDK NIH HHS
        • HD 00412 / NICHD NIH HHS

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        Citations

        This article has been cited 3 times.
        1. Nilson R, Lübbers O, Weiß L, Singh K, Scharffetter-Kochanek K, Rojewski M, Schrezenmeier H, Zeplin PH, Funk W, Krutzke L, Kochanek S, Kritzinger A. Transduction Enhancers Enable Efficient Human Adenovirus Type 5-Mediated Gene Transfer into Human Multipotent Mesenchymal Stromal Cells. Viruses 2021 Jun 12;13(6).
          doi: 10.3390/v13061136pubmed: 34204818google scholar: lookup
        2. Parchment RE, Natarajan K. A free-radical hypothesis for the instability and evolution of genotype and phenotype in vitro. Cytotechnology 1992;10(2):93-124.
          doi: 10.1007/BF00570888pubmed: 1369214google scholar: lookup
        3. Šebela M, Rašková M. Polyamine-Derived Aminoaldehydes and Acrolein: Cytotoxicity, Reactivity and Analysis of the Induced Protein Modifications. Molecules 2023 Nov 4;28(21).
          doi: 10.3390/molecules28217429pubmed: 37959847google scholar: lookup
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