Susceptibility of equine chorionic girdle cells to lymphokine-activated killer cell activity.
Abstract: Equine chorionic girdle cells are a subpopulation of highly invasive trophoblast cells that attach and invade the uterine epithelium on Day 35 (Day 0 = day of ovulation). These invading chorionic girdle cells form endometrial cups that are associated with a marked local maternal leukocytic response that may result in the demise of the cups at Day 120 of pregnancy. Once endometrial cups become established in the uterine wall they do not express MHC antigens, and therefore may only be susceptible to non-MHC restricted cytotoxic cells. The susceptibility of cultured chorionic girdle cells to LAK cell cytotoxicity was tested in order to evaluate the role of this type of cytotoxicity in the life-cycle of endometrial cup tissue. Methods: Chorionic girdle cells from ten Day 34 conceptuses were collected, cultured, and used as a target cell in a lymphokine activated killer assay to determine if these cells were susceptible to lymphokine activated killing. Results: Cultured chorionic girdle cells demonstrated similar in vitro morphological features and patterns of antigen expression to those seen in vivo in endometrial cups. Cultured chorionic girdle cells were susceptible to lymphokine activated killing. Conclusions: Lymphokine activated killer cells may play a role in the degeneration of endometrial cup tissue. The potential mechanisms of regulation of uterine LAK cell activity in the horse are discussed.
Publication Date: 1996-09-01 PubMed ID: 8874715DOI: 10.1111/j.1600-0897.1996.tb00160.xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article investigates the susceptibility of equine chorionic girdle cells (a type of cell involved in horse pregnancy) to lymphokine-activated killer (LAK) cell activity, revealing that these cells are susceptible to such activity. This suggests a possible role for LAK cells in the life-cycle of horse pregnancy-related tissues.
Objective of the Research
- This research aims to examine the susceptibility of equine chorionic girdle cells – a subset of invasive trophoblast cells critical to horse pregnancy – to the cytotoxic activity of LAK cells.
About Chorionic Girdle Cells
- These cells attach and invade the uterine epithelium approximately 35 days after ovulation (taken as Day 0) in horses. They form endometrial cups, associated with a strong local maternal leukocytic response, in the uterine wall that may result in the death of the cups around Day 120 of pregnancy.
- Once established, the endometrial cups do not express major histocompatibility complex (MHC) antigens, suggesting their susceptibility to non-MHC restricted cytotoxic cells – hence the focus on LAK cell activity.
Methods Used in the Research
- Researchers collected chorionic girdle cells from ten Day 34 conceptuses (embryos in the stage of pregnancy where major cellular differentiation begins), cultured them, and used them as a target in a lymphokine activated killer assay to see if they would be susceptible to LAK cell killing.
Results of the Research
- When cultivated, the chorionic girdle cells displayed similar in vitro morphological characteristics and antigen expression patterns as they would in vivo in endometrial cups.
- The chorionic girdle cells in culture proved to be susceptible to lymphokine-activated killing, demonstrating the influence of LAK cells.
Conclusions of the Research
- The study concludes that LAK cells may have a role to play in the breakdown of endometrial cup tissue and discuss potential mechanisms by which uterine LAK cell activity could be regulated in horses.
Cite This Article
APA
Vagnoni KE, Schram BR, Ginther OJ, Lunn DP.
(1996).
Susceptibility of equine chorionic girdle cells to lymphokine-activated killer cell activity.
Am J Reprod Immunol, 36(3), 184-190.
https://doi.org/10.1111/j.1600-0897.1996.tb00160.x Publication
Researcher Affiliations
- Department of Obstetrics and Gynecology, Meriter Hospital, University of Wisconsin, Madison 53715, USA.
MeSH Terms
- Animals
- Chorion / cytology
- Chorion / immunology
- Cytotoxicity, Immunologic
- Horses
- Immunity, Innate
- Killer Cells, Lymphokine-Activated / immunology
- Trophoblasts / immunology
Citations
This article has been cited 2 times.- Rapacz-Leonard A, Dąbrowska M, Janowski T. Major histocompatibility complex I mediates immunological tolerance of the trophoblast during pregnancy and may mediate rejection during parturition.. Mediators Inflamm 2014;2014:579279.
- Futas J, Horin P. Natural killer cell receptor genes in the family Equidae: not only Ly49.. PLoS One 2013;8(5):e64736.
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