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Journal of virology1994; 68(8); 4890-4897; doi: 10.1128/JVI.68.8.4890-4897.1994

The extreme carboxyl terminus of the equine herpesvirus 1 homolog of herpes simplex virus VP16 is essential for immediate-early gene activation.

Abstract: Gene 12 of equine herpesvirus 1 (EHV-1), the homolog of herpes simplex virus (HSV) VP16 (alpha TIF, Vmw65), was cloned into a eukaryotic expression vector by PCR and used in transactivation studies of both the EHV-1 and HSV-1 IE1 promoters. Results demonstrated that the product of gene 12 is a potent transactivator of immediate-early gene expression of both viruses, which requires sequences in the upstream HSV-1 promoter for activity. Mutational analysis of the gene 12 open reading frame indicated that removal of the C-terminal 7 amino acids, which contain a short region of homology with the extreme C terminus of VP16, inactivated the protein. Within this region, only a single methionine residue appeared to be essential for activity, implying that gene 12 may have a modular array of organization similar to that of VP16. However, fusion of the gene 12 C terminus to a truncated form of VP16, which contained the complex formation domain, did not restore activity to the HSV-1 protein. These data demonstrate that the EHV-1 immediate-early transactivator may not be functionally colinear with VP16, with transactivation requiring both the C terminus and another region(s) present within the N-terminal portion.
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Publication Date: 1994-08-01 PubMed ID: 8035487PubMed Central: PMC236429DOI: 10.1128/JVI.68.8.4890-4897.1994Google Scholar: Lookup
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper discusses a study on the gene 12 of equine herpesvirus 1 (EHV-1) and its role in the immediate-early gene expression of both EHV-1 and herpes simplex virus (HSV-1), as well as the importance of a single methionine residue in the C-terminal region of this gene.

Study on Gene 12 of Equine Herpesvirus 1 (EHV-1)

  • The researchers cloned gene 12 of EHV-1, a homolog of herpes simplex virus (HSV) VP16, into a eukaryotic expression vector using PCR.
  • The goal was to use this cloning in transactivation studies of the EHV-1 and HSV-1 IE1 promoters.
  • The finding revealed that the product of gene 12 acts as a potent transactivator of immediate-early gene expression for both viruses.
  • It was found that the activity of this gene product requires sequences in the upstream HSV-1 promoter.

Mutational Analysis of Gene 12

  • The researchers carried out a mutational analysis on the open reading frame of gene 12.
  • They found that by removing the C-terminal 7 amino acids, which contain a short region of homology with the extreme C terminus of VP16, resulted in the inactivation of the protein.
  • Within this region, only a single methionine residue appeared to be essential for activity, suggesting that gene 12 may be organized similarly to VP16.

Comparing Functionality of EHV-1 with VP16

  • In the study, it was observed that mere fusion of the gene 12 C terminus to a truncated form of VP16 did not restore activity to the HSV-1 protein.
  • This implies that the EHV-1 immediate-early transactivator might not be functionally equivalent to VP16.
  • Transactivation requires both the C terminus and another region(s) present within the N-terminal portion of the molecular structure.

Cite This Article

APA
Elliott GD. (1994). The extreme carboxyl terminus of the equine herpesvirus 1 homolog of herpes simplex virus VP16 is essential for immediate-early gene activation. J Virol, 68(8), 4890-4897. https://doi.org/10.1128/JVI.68.8.4890-4897.1994

Publication

Journal of virology
ISSN: 0022-538X
NlmUniqueID: 0113724
Country: United States
Language: English
Volume: 68
Issue: 8
Pages: 4890-4897

Researcher Affiliations

Elliott, G D
  • Department of Microbiology, University of Leeds, United Kingdom.

MeSH Terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Cloning, Molecular
  • DNA, Viral
  • Gene Expression Regulation, Viral
  • Herpes Simplex Virus Protein Vmw65 / chemistry
  • Herpes Simplex Virus Protein Vmw65 / genetics
  • Herpesvirus 1, Equid / genetics
  • Immediate-Early Proteins / genetics
  • Methionine / metabolism
  • Molecular Sequence Data
  • Promoter Regions, Genetic
  • Sequence Homology
  • Transcriptional Activation
  • Vero Cells

Grant Funding

  • Wellcome Trust

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