The influence of age on serum concentrations of cardiac troponin I: results in rats, monkeys, and commercial sera.
Abstract: Cardiac troponins serve as serum biomarkers of myocardial injury. The current study examined the influence of age on serum concentrations of cardiac troponin I (cTnI). An ultrasensitive immunoassay was used to monitor cTnI concentrations in Sprague-Dawley (SD) rats and Erythrocebus patas monkeys of different ages. The mean cTnI concentrations were highest in 10-day-old rats compared to 25-, 40-, and 80-day-old SD rats. Cardiomyocyte remodeling was apparent in hearts from 10-day-old SD rats as evident by hypercellularity, irregularly shaped nuclei, and moderate numbers of myocytes undergoing mitosis and apoptosis. The mean concentration of cTnI in 5 newborn monkeys was considerably higher than that of three 1-year-old monkeys. Evidence of cardiomyocyte remodeling was also observed in these newborn hearts (loss of myofibrils and cytoplasmic vacuolation). Commercial animal serum samples were also analyzed. The concentrations of cTnI detected in fetal equine and porcine serum were considerably higher than that found in adult equine and porcine serum samples Likewise, fetal bovine serum had higher cTnI concentrations (>2,400 pg/ml) than did adult caprine and laprine samples (2.5-2.7 pg/ml). The present study found age-related differences in cTnI concentrations, with higher levels occurring at younger ages. This effect was consistent across several animal species.
© 2014 by The Author(s).
Publication Date: 2013-10-15 PubMed ID: 24129761DOI: 10.1177/0192623313505154Google Scholar: Lookup
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- Journal Article
Summary
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The research article investigates the relationship between age and the levels of a heart damage marker, cardiac troponin I (cTnI), in various animal species. The findings highlight that younger animals, particularly in their neonatal stages, have higher concentrations of cTnI, hinting at age-related differences.
Study Design and Species Involved
- The research team used an ultrasensitive immunoassay to measure cTnI concentrations in Sprague-Dawley (SD) rats and Erythrocebus patas monkeys of varying ages.
- Also included in the research were commercial serum samples from different animal species including equine (horse), porcine (pig), bovine (cow), caprine (goat), and laprine (rabbit).
Findings in Rats
- Among the SD rats, it was revealed that the average cTnI concentration was highest in ten-day-old rats compared to those aged 25, 40, and 80 days.
- Visual examination showed signs of cardiomyocyte (heart muscle cell) remodeling in the hearts of ten-day-old rats. This remodeling process includes changes such as hypercellularity (increase in cell quantity), irregular nuclear shape, and a moderate number of myocytes undergoing mitosis (cell division) and apoptosis (programmed cell death).
Findings in Monkeys
- Similarly, newborn monkeys exhibited significantly higher concentrations of cTnI as compared to one-year-old monkeys.
- These newborns also showed signs of cardiomyocyte remodeling, including loss of myofibrils (muscle fiber components) and cytoplasmic vacuolation (cellular process creating small cavities).
Findings in Commercial Animal Sera
- The researchers further analyzed commercial animal serum samples and found a similar trend.
- In equine and porcine, fetal serum had higher concentrations of cTnI as compared to adult serum samples.
- In bovine, fetal serum showed cTnI levels greater than 2,400 pg/ml, substantially higher than adult caprine and laprine samples that ranged between 2.5 to 2.7 pg/ml.
Conclusion
- By examining cTnI concentrations across different species and ages, the study concludes an age-related difference with younger ages reflecting higher concentrations.
- This observation remained consistent across different animal species.
- Such findings may provide essential insights into the cardiovascular health and development of various species, serving as a foundation for future research and medical treatment approaches.
Cite This Article
APA
Herman EH, Knapton A, Liu Y, Lipshultz SE, Estis J, Todd J, Woodward RA, Cochran T, Zhang J, Poirier MC.
(2013).
The influence of age on serum concentrations of cardiac troponin I: results in rats, monkeys, and commercial sera.
Toxicol Pathol, 42(5), 888-896.
https://doi.org/10.1177/0192623313505154 Publication
Researcher Affiliations
- Food and Drug Administration, Division of Drug Safety Research, Silver Spring, Maryland, USA eugene.herman@fda.hhs.gov.
- Food and Drug Administration, Division of Drug Safety Research, Silver Spring, Maryland, USA.
- National Institutes of Health, National Cancer Institute, Carcinogen-DNA Interactions Section, Bethesda, Maryland, USA.
- Department of Pediatrics, Leonard M. Miller School of Medicine, Mailman Center for Child Development, University of Miami, Miami, Florida, USA.
- Singulex, Inc., Alameda, California, USA.
- Singulex, Inc., Alameda, California, USA.
- Shared Animal Facility, NIH Animal Center, Dickerson, Maryland, USA.
- Department of Pediatrics, Leonard M. Miller School of Medicine, Mailman Center for Child Development, University of Miami, Miami, Florida, USA.
- Food and Drug Administration, Division of Drug Safety Research, Silver Spring, Maryland, USA.
- National Institutes of Health, National Cancer Institute, Carcinogen-DNA Interactions Section, Bethesda, Maryland, USA.
MeSH Terms
- Age Factors
- Animals
- Biomarkers / blood
- Cattle
- Erythrocebus patas
- Female
- Heart Injuries / blood
- Horses
- Immunoassay
- Male
- Myocardium / metabolism
- Myofibrils / metabolism
- Rats
- Rats, Sprague-Dawley
- Swine
- Troponin I / blood
Citations
This article has been cited 1 times.- Lipshultz SE, Herman EH. Anthracycline cardiotoxicity: the importance of horizontally integrating pre-clinical and clinical research.. Cardiovasc Res 2018 Feb 1;114(2):205-209.
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