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Home / Equine Research Bank / Sci Rep / The myometrial transcriptome changes in mares with endometro...
Scientific reports2025; 15(1); 3173; doi: 10.1038/s41598-025-86742-0

The myometrial transcriptome changes in mares with endometrosis.

Abstract: Mares with endometrosis exhibit histological changes not only in the endometrium but also in the myometrium that suggest possible functional impairment. The molecular background of these changes is not well understood. We hypothesize that the transcriptomic profile of the mare myometrium varies depending on the degree of endometrosis in mares. Myometria were collected from mares in the mid-luteal phase of the estrous cycle with endometrium categories I, IIA, IIB, and III (∑n = 23), according to Kenney and Doig´s histopathological classification. Myometrial RNA was isolated and subjected to RNA-seq analysis to identify differentially expressed transcriptionally active regions (deTARs) and their contribution to signaling pathways (KEGG database) and biological processes (GO terms). In results, 665, 491 and 499 deTARs were found in the myometrium of mares with endometrium IIA vs I, IIB vs I and III vs I, respectively. 200 common deTARs in the myometrium across all stages of endometrosis (IIA, IIB, and III) vs I were identified. Evaluated deTARs enriched several KEGG pathways including calcium signaling, cAMP signaling, oxytocin signaling, ECM-receptor interaction, and focal adhesion, and were classified into various GO terms including adaptive immune response, tissue homeostasis, muscle contractions, muscle development, and other. In conclusion, transcriptomic alterations in the myometrium of mares with endometrosis may indicate an impaired function of the contractile machinery, mechanisms regulating calcium influx and handling, as well as changes in ECM composition, leading to a decreased contractile activity and structural changes in the myometrium of affected mares.
© 2025. The Author(s).
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Publication Date: 2025-01-25 PubMed ID: 39863666PubMed Central: PMC11762791DOI: 10.1038/s41598-025-86742-0Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research aims to understand the changes in the myometrial transcriptome in mares with endometrosis, a uterine disease, by conducting histopathological classifications, RNA-seq analysis, and enrichment of KEGG pathways and GO terms.

Objective of the research

  • The goal of this research was to determine how varying degrees of endometrosis, a uterine disease in mares, affect the transcriptome profile of the myometrium. Test samples were obtained from the myometria of mares in their estrous cycle’s mid-luteal phase.

Research Methodology

  • The test samples included mares with different categories of endometrium. The histopathological classification from Kenney and Doig was used to define these categories as I, IIA, IIB, and III.
  • The RNA in the myometrium was isolated and examined using RNA-seq analysis. This enabled the researchers to identify differentially expressed transcriptionally active regions (deTARs).
  • The researchers then looked at the impact of these deTARs on signaling pathways from the KEGG database, and in the context of the GO terms that represent biological processes.

Key Findings

  • The study found different numbers of deTARs in comparison pairs of myometrium categories, with 665 in IIA vs I, 491 in IIB vs I, and 499 in III vs I.
  • The researchers identified 200 common deTARs in the myometrium across all stages of endometrosis (IIA, IIB, and III) in comparison with stage I.
  • The deTARs enriched several KEGG pathways (calcium signaling, cAMP signaling, oxytocin signaling, ECM-receptor interaction, and focal adhesion) and were categorized into various GO terms (adaptive immune response, tissue homeostasis, muscle contractions, muscle development, and more).

Conclusions

  • The study concluded that there are transcriptomic alterations in the myometrium of mares with endometrosis. These changes could suggest impaired function of the contractile machinery, issues with regulation of calcium influx and handling, and changes in ECM composition.
  • These alterations can lead to decreased contractile activity and structural changes within the myometrium of affected mares, potentially providing insights into the functional changes observed in endometrosis.

Cite This Article

APA
Drzewiecka EM, Molcan T, Sadowska A, Piotrowska-Tomala K, Słyszewska M, Dias GF, Skarżyński DJ, Szóstek-Mioduchowska A. (2025). The myometrial transcriptome changes in mares with endometrosis. Sci Rep, 15(1), 3173. https://doi.org/10.1038/s41598-025-86742-0

Publication

Scientific reports
ISSN: 2045-2322
NlmUniqueID: 101563288
Country: England
Language: English
Volume: 15
Issue: 1
Pages: 3173
PII: 3173

Researcher Affiliations

Drzewiecka, Ewa Monika
  • Institute of Animal Reproduction and Food Research, Polish Academy of Sciences in Olsztyn, 10-748, Olsztyn, Poland. e.drzewiecka@pan.olsztyn.pl.
Molcan, Tomasz
  • Molecular Biology Laboratory, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences in Olsztyn, 10-748, Olsztyn, Poland.
Sadowska, Agnieszka
  • Institute of Animal Reproduction and Food Research, Polish Academy of Sciences in Olsztyn, 10-748, Olsztyn, Poland.
Piotrowska-Tomala, Katarzyna
  • Institute of Animal Reproduction and Food Research, Polish Academy of Sciences in Olsztyn, 10-748, Olsztyn, Poland.
Słyszewska, Magda
  • Institute of Animal Reproduction and Food Research, Polish Academy of Sciences in Olsztyn, 10-748, Olsztyn, Poland.
Dias, Graça Ferreira
  • Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, 1300-477, Lisbon, Portugal.
  • Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), 1300-477, Lisbon, Portugal.
Skarżyński, Dariusz Jan
  • Institute of Animal Reproduction and Food Research, Polish Academy of Sciences in Olsztyn, 10-748, Olsztyn, Poland.
Szóstek-Mioduchowska, Anna
  • Institute of Animal Reproduction and Food Research, Polish Academy of Sciences in Olsztyn, 10-748, Olsztyn, Poland.

MeSH Terms

  • Female
  • Animals
  • Horses
  • Myometrium / metabolism
  • Myometrium / pathology
  • Transcriptome
  • Endometriosis / genetics
  • Endometriosis / metabolism
  • Endometriosis / pathology
  • Endometriosis / veterinary
  • Horse Diseases / genetics
  • Horse Diseases / metabolism
  • Gene Expression Profiling
  • Endometrium / metabolism
  • Endometrium / pathology
  • Signal Transduction

Grant Funding

  • 2022/06/X/NZ5/01258 / National Science Centre, Poland.

Conflict of Interest Statement

Declarations. Competing interests: The authors declare no competing interests.

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