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Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology2002; 10(7); 571-577; doi: 10.1023/a:1020914702767

The use of laser microdissection for the preparation of chromosome-specific painting probes in farm animals.

Abstract: Laser microbeam microdissection and laser pressure catapulting procedure were used for the construction of chromosome-specific painting probes, arm-specific probes and probes for chromosomal subfragments. We report on a method for generation of fluorescence in-situ hybridization probes from laser dissected chromosomes of farm animals. So far, using the described method, a set of chromosome-specific painting probes has been obtained for all porcine chromosomes, 17 chromosomes of cattle and selected equine chromosomes. It is concluded that the laser technology appears to be a useful and powerful tool for the construction of chromosome-specifi c painting probes. Its main advantage is the fast non-contact collection of chromosomes.
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Publication Date: 2002-12-25 PubMed ID: 12498346DOI: 10.1023/a:1020914702767Google Scholar: Lookup
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  • Evaluation Study
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research discusses a procedure using laser microdissection to create specific probes for examining chromosomes in farm animals. These probes can help in the creation of a detailed genetic map.

Objective and Method

The main objective of this study was to develop a method for generating fluorescence in-situ hybridization (FISH) probes from the chromosomes of farm animals. The researchers utilized two specific laser technologies, namely laser microbeam microdissection and laser pressure catapulting, for constructing probe types such as chromosome-specific painting probes, arm-specific probes, and probes for chromosomal subfragments. The entire process is non-contact, meaning it doesn’t require physical interaction with the sample, and it’s considerably quicker compared to traditional methods.

  • Laser Microbeam Microdissection: This is a method used in molecular biology research to obtain specific subsets of tissue for analysis, like specific chromosomes in this case.
  • Laser Pressure Catapulting: This technique is used specifically to collect the dissected chromosomes.

Results and Achievements

By employing this novel method, researchers managed to obtain a set of chromosome-specific painting probes for all porcine (pig) chromosomes, 17 chromosomes of cattle and several specific equine (horse) chromosomes. The adjective “painting” refers to the fact that these probes are designed to bind to specific areas of the chromosome so they can be easily identified.

Conclusions

The study concluded that laser technology is notably effective and efficient in constructing chromosome-specific painting probes. It allows for the fast, non-contact collection of chromosomes which ultimately speeds up and simplifies the process of genetic research in farm animals.

Cite This Article

APA
Kubickova S, Cernohorska H, Musilova P, Rubes J. (2002). The use of laser microdissection for the preparation of chromosome-specific painting probes in farm animals. Chromosome Res, 10(7), 571-577. https://doi.org/10.1023/a:1020914702767

Publication

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology
ISSN: 0967-3849
NlmUniqueID: 9313452
Country: Netherlands
Language: English
Volume: 10
Issue: 7
Pages: 571-577

Researcher Affiliations

Kubickova, Svatava
  • Veterinary Research Institute, Hudcova 70, 621 32 Brno, Czech Republic.
Cernohorska, Halina
    Musilova, Petra
      Rubes, Jiri

        MeSH Terms

        • Animals
        • Animals, Domestic / genetics
        • Cattle
        • Chromosome Banding
        • Chromosome Painting / methods
        • Chromosomes / genetics
        • DNA Probes
        • Dissection / methods
        • Equidae
        • Genetic Markers
        • Heterozygote
        • In Situ Hybridization, Fluorescence
        • Karyotyping
        • Lasers
        • Species Specificity
        • Swine
        • Taq Polymerase / genetics
        • Translocation, Genetic

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