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Home / Equine Research Bank / Int J Mol Sci / Therapeutic Potential in Wound Healing of Allogeneic Use of ...
International journal of molecular sciences2024; 25(4); doi: 10.3390/ijms25042350

Therapeutic Potential in Wound Healing of Allogeneic Use of Equine Umbilical Cord Mesenchymal Stem Cells.

Abstract: Wound healing after skin injury is a complex process, particularly in equines where leg wounds are prevalent and their repair is complicated due to the anatomical characteristics. Conventional treatments are not effective enough. The umbilical cord offers an unlimited source of adult mesenchymal stem cells (ucMSCs) from Wharton's jelly tissue. The present study aims to demonstrate the safety and therapeutic potential of the allogeneic use of equine ucMSCs (e-ucMSCs) in the healing of severe equine leg wounds. The methods employed were the isolation, culture and expansion of e-ucMSCs. Flow cytometry and a PCR assay were used for cell characterization. This study included an immunomodulation assay, a murine pre-clinical trial and the first phase of an equine clinical trial. Our results showed that e-ucMSCs express a functional HLA-G homolog, EQMHCB2. In the immunomodulation assay, the e-ucMSCs inhibited the proliferation of activated equine peripheral blood mononuclear cells (e-PBMCs). In the murine pre-clinical trial, e-ucMSCs reduced healing time by 50%. In the equine clinical trial, the injection of e-ucMSCs into severe leg lesions improved the closure time and quality of the tissues involved, regenerating them without fibrous tissue scar formation. In conclusion, the results of this study suggest that e-ucMSCs can be used allogeneically for wound healing by creating a tolerogenic environment.
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Publication Date: 2024-02-16 PubMed ID: 38397024PubMed Central: PMC10889822DOI: 10.3390/ijms25042350Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article outlines an experiment which studies the potential of using equine umbilical cord stem cells (e-ucMSCs) in healing severe leg injuries in horses. The study found that these stem cells can indeed promote quicker and higher quality wound healing, suggesting their possible future use in medical applications.

Objective and Methodology

  • The research seeks to find more effective treatments for wound healing in horses, particularly those injuries on the leg which can be complex due to the animal’s specific anatomy.
  • The team hypothesizes that mesenchymal stem cells (MSCs), derived from the Wharton’s jelly tissue of the equine umbilical cord, could potentially help in wound recovery.
  • The methodology employed for the study includes the isolation, culture, and proliferation of the umbilical cord stem cells. The team also performed characterization assays on the cells through flow cytometry and a Polymerase Chain Reaction (PCR) assay.

Immunomodulation Assay, Preclinical Trial and Clinical Trial

  • An immunomodulation assay was conducted to test how the stem cells interact with the immune cells of the horses. It was found that e-ucMSCs inhibited the proliferation of activated horse immune cells, creating a more tolerogenic or accepting environment for recovery.
  • A preclinical trial was conducted on mice where the use of stem cells resulted in a reduction of healing time by around 50%, showcasing the stem cells’ potential efficacy.
  • The first phase of a clinical trial on horses was also conducted. The stem cells were injected into severe leg lesions where it was observed that the healing time of the wounds was improved and the quality of tissue regeneration also enhanced, with no fibrous scar tissue formation.

Conclusion

  • The researchers concluded that e-ucMSCs could be used to improve wound healing in horses, creating a tolerogenic or accepting environment for recovery. This suggests a promising future in the use of e-ucMSCs for wound healing applications in equine medical settings.

Cite This Article

APA
Iribarne A, Palma MB, Andrini L, Riccillo F, Rodriguez D, Casella M, Garay F, Zabala JS, Mazza L, Muro A, Buero G, Miriuka SG, Carosella E, García MN. (2024). Therapeutic Potential in Wound Healing of Allogeneic Use of Equine Umbilical Cord Mesenchymal Stem Cells. Int J Mol Sci, 25(4). https://doi.org/10.3390/ijms25042350

Publication

International journal of molecular sciences
ISSN: 1422-0067
NlmUniqueID: 101092791
Country: Switzerland
Language: English
Volume: 25
Issue: 4

Researcher Affiliations

Iribarne, Ailén
  • Cátedra de Citología, Histología y Embriología, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, Buenos Aires CP 1900, Argentina.
Palma, María Belén
  • Cátedra de Citología, Histología y Embriología, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, Buenos Aires CP 1900, Argentina.
  • Instituto de Neurociencias (INEU), Fundación para la Lucha contra Enfermedades Neurológicas de la Infancia-Consejo Nacional de Investigaciones Científicas y Técnicas (FLENI-CONICET) Escobar, Buenos Aires CP B1625, Argentina.
Andrini, Laura
  • Cátedra de Citología, Histología y Embriología, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, Buenos Aires CP 1900, Argentina.
Riccillo, Fernando
  • Cátedra de Citología, Histología y Embriología, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, Buenos Aires CP 1900, Argentina.
Rodriguez, Delfina
  • Centro de Reproducción Equina La Adela, Chascomús, Buenos Aires CP 7130, Argentina.
Casella, Martín
  • División Remonta Haras La Teruca, Buenos Aires B1900, Argentina.
Garay, Felipe
  • División Remonta Haras La Teruca, Buenos Aires B1900, Argentina.
  • Dirección Veterinaria, Policía de la Provincia de Buenos Aires, Buenos Aires B1904, Argentina.
Zabala, Julieta Spoto
  • División Remonta Haras La Teruca, Buenos Aires B1900, Argentina.
  • Dirección Veterinaria, Policía de la Provincia de Buenos Aires, Buenos Aires B1904, Argentina.
Mazza, Leandro
  • Cátedra de Anatomía B, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, Buenos Aires CP 1900, Argentina.
Muro, Adriana
  • Laboratorio de Análisis Clínicos Veterinarios, La Plata, Buenos Aires CP 1900, Argentina.
Buero, Guillermo
  • Sanatorio Mater Dei, Ciudad Autónoma de Buenos Aires, Buenos Aires CP C1425, Argentina.
Miriuka, Santiago G
  • Instituto de Neurociencias (INEU), Fundación para la Lucha contra Enfermedades Neurológicas de la Infancia-Consejo Nacional de Investigaciones Científicas y Técnicas (FLENI-CONICET) Escobar, Buenos Aires CP B1625, Argentina.
Carosella, Edgardo
  • Commissariat a l'Energie Atomique et aux Energies Alternatives (CEA), Service de Recherche en Hemato-Immunologie (SRHI), Saint-Louis Hospital, 75010 Paris, France.
  • Hopital Saint-Louis, IUH, Université Paris Diderot, Sorbonne Paris Cite, 75010 Paris, France.
García, Marcela N
  • Cátedra de Citología, Histología y Embriología, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, Buenos Aires CP 1900, Argentina.

MeSH Terms

  • Animals
  • Horses
  • Mice
  • Leukocytes, Mononuclear
  • Mesenchymal Stem Cells
  • Umbilical Cord
  • Cicatrix
  • Hematopoietic Stem Cell Transplantation

Conflict of Interest Statement

The authors declare no conflicts of interest.

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