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Reproduction in domestic animals = Zuchthygiene2024; 59 Suppl 3; e14657; doi: 10.1111/rda.14657

TLR-2 and TLR-4 mRNA expression in different grades of histopathological lesions of equine endometrium from follicular phase.

Abstract: Increased synthesis and deposition of collagen (COL) in the extracellular matrix (ECM) of equine endometrium contributes to endometrosis. Toll-like receptors (TLRs) are transmembrane receptors involved in the innate immune response, recognized for their role in antigen recognition and previously associated with equine endometritis. The TLRs not only recognize pathogen-associated molecular patterns but also regulate inflammations, fibrosis and cancer. The aim of this study was to explore the relationship between TLR expression at different stages of Kenney and Doig's (K-D) grading and COL1 expression during the follicular phase of the oestrous cycle. Forty samples of endometrial tissues were collected post-mortem from mares on the follicular phase of the oestrous cycle (10 samples of each K-D category). Relative mRNA transcription of TLR-2, TLR-4 and COL1A2 genes was assessed using qPCR, and COL1 protein expression by Western blot analysis. The COL1A2 transcription increased in category IIB when compared to categories I, IIA and III endometria (p  .05). As for the TLRs mRNA transcription, TLR-2/-4 transcripts increased in IIA when compared to the other K-D endometria categories (p < .05). Our findings suggest that TLRs may be involved in the initiation of the endometrial inflammatory response. Additional studies are needed to explore TLRs' potential role as diagnostic markers for monitoring inflammation progression and fibrosis development, as well as their involvement in the mechanisms underlying fibrotic pathways.
© 2024 Wiley‐VCH GmbH. Published by John Wiley & Sons Ltd.
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Publication Date: 2024-10-14 PubMed ID: 39396880DOI: 10.1111/rda.14657Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study examines how Toll-like receptors (TLRs) and collagen (COL) synthesis relate to the progression of endometrial inflammation in horses. Researchers found that certain TLRs increase during certain stages of inflammation, it could mean that TLRs have a significant role in the initiation of the inflammation process.

Objective

The study aimed to understand the role of TLRs and their association with the synthesis and deposition of COL in the Equine endometrium, especially in relation to the progression of inflammation or endometrosis. The researchers specifically focused on TLR-2 and TLR-4 mRNA transcription and its variation across different stages of inflammatory response.

Methodology

  • Researchers collected endometrial tissues from horses post-mortem. These sampled tissues were in the follicular phase of the oestrous cycle.
  • They categorized the samples into four groups according to the K-D (Kenney and Doig’s) category of the inflammation progress.
  • The genes TLR-2, TLR-4 and COL1A2 were then analyzed and compared across these different categories using a method called qPCR.
  • The protein abundance of COL1 was assessed through a technique known as Western blot analysis.

Findings

  • They observed that the transcription of COL1A2 increased in K-D category IIB as compared to categories I, IIA, and III.
  • On comparing the protein abundance of COL1 across different K-D categories, the researchers found no significant differences.
  • The transcription of TLR-2/-4 increased in IIA compared to the other K-D categories. This suggested that TLRs could have a role in the initiation of the inflammatory response in the endometrium.

Implications and Future Directions

  • The study’s results imply that TLRs may be integral to the initiation of the endometrial inflammatory response. This could offer potential insight into the treatment and prevention of endometrosis in horses.
  • However, more research is needed to ascertain the potential role of TLRs as diagnostic markers for monitoring inflammation progression, fibrosis development and to understand their role in fibrotic pathways.

Cite This Article

APA
Cerveira-Pinto M, Wójtowicz A, Pires MA, Kordowitzki P, Skarzynski D, Ferreira-Dias G, Szóstek-Mioduchowska A, Amaral A. (2024). TLR-2 and TLR-4 mRNA expression in different grades of histopathological lesions of equine endometrium from follicular phase. Reprod Domest Anim, 59 Suppl 3, e14657. https://doi.org/10.1111/rda.14657

Publication

Reproduction in domestic animals = Zuchthygiene
ISSN: 1439-0531
NlmUniqueID: 9015668
Country: Germany
Language: English
Volume: 59 Suppl 3
Pages: e14657

Researcher Affiliations

Cerveira-Pinto, Marta
  • CIISA-Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, Lisbon, Portugal.
  • Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), Lisbon, Portugal.
Wójtowicz, Anna
  • Institute of Animal Reproduction and Food Research, Polish Academy of Science, Olsztyn, Poland.
Pires, Maria Anjos
  • Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), Lisbon, Portugal.
  • CECAV-Animal and Veterinary Research Centre, University of Trás-os-Montes e Alto Douro (UTAD), Vila Real, Portugal.
Kordowitzki, Pawel
  • Department of Basic and Preclinical Sciences, Institute for Veterinary Medicine, Nicolaus Copernicus University, Torun, Poland.
Skarzynski, Dariusz
  • Department of Reproduction and Clinic of Farm Animals, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland.
Ferreira-Dias, Graça
  • CIISA-Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, Lisbon, Portugal.
  • Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), Lisbon, Portugal.
Szóstek-Mioduchowska, Anna
  • Institute of Animal Reproduction and Food Research, Polish Academy of Science, Olsztyn, Poland.
Amaral, Ana
  • CIISA-Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, Lisbon, Portugal.
  • Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), Lisbon, Portugal.
  • Comprehensive Health Research Centre (CHRC), Évora, Portugal.

MeSH Terms

  • Animals
  • Horses
  • Female
  • Endometrium / metabolism
  • Endometrium / pathology
  • Toll-Like Receptor 4 / genetics
  • Toll-Like Receptor 4 / metabolism
  • Toll-Like Receptor 2 / genetics
  • Toll-Like Receptor 2 / metabolism
  • RNA, Messenger / metabolism
  • RNA, Messenger / genetics
  • Horse Diseases / genetics
  • Horse Diseases / metabolism
  • Horse Diseases / pathology
  • Follicular Phase
  • Collagen Type I / genetics
  • Collagen Type I / metabolism
  • Endometritis / veterinary
  • Endometritis / metabolism
  • Endometritis / pathology
  • Endometritis / genetics

Grant Funding

  • UIDB/00276/2020 / Fundau00e7u00e3o para a Ciu00eancia e a Tecnologia
  • LA/P/0059/2020 / Fundau00e7u00e3o para a Ciu00eancia e a Tecnologia
  • 2022.07400.PTDC / Fundau00e7u00e3o para a Ciu00eancia e a Tecnologia
  • 2023.04679.BD / Fundau00e7u00e3o para a Ciu00eancia e a Tecnologia
  • BPN/BPT/2021/1/00026/U/00001 / Poland NAWA Project
  • FBW/8/2023 / IAR&FR project

References

This article includes 22 references
  1. Alpoim‐Moreira J, Fernandes C, Rebordão M R, Costa A L, Bliebernicht M, Nunes T, Szóstek‐Mioduchowska A, Skarzynski D J, Ferreira‐Dias G. Collagen type III as a possible blood biomarker of fibrosis in equine endometrium. Animals 12(14), 1854.
    doi: 10.3390/ani12141854google scholar: lookup
  2. Amaral A, Fernandes C, Rebordão M R, Szóstek‐Mioduchowska A, Lukasik K, Gawronska‐Kozak B, Telo Da Gama L, Skarzynski D J, Ferreira‐Dias G. The in vitro inhibitory effect of sivelestat on elastase induced collagen and metallopeptidase expression in equine endometrium. Animals 10(5), 863.
    doi: 10.3390/ani10050863google scholar: lookup
  3. Bolourani S, Brenner M, Wang P. The interplay of DAMPs, TLR4, and proinflammatory cytokines in pulmonary fibrosis. Journal of Molecular Medicine 99(10), 1373–1384.
    doi: 10.1007/s00109‐021‐02113‐ygoogle scholar: lookup
  4. Centeno L A M, Bastos H B, Bueno V L, Trentin J M, Larentis G R, Fiorenza M F, Panziera W, Winter G H Z, Fiala‐Rechsteiner S, Mattos R C, Rubin M I B. Endometrial fibrosis in mares: Picrosirius Red and Collagen‐2 and 3. Journal of Equine Veterinary Science 125, 104699.
    doi: 10.1016/j.jevs.2023.104699google scholar: lookup
  5. Domon H, Nagai K, Maekawa T, Oda M, Yonezawa D, Takeda W, Hiyoshi T, Tamura H, Yamaguchi M, Kawabata S, Terao Y. Neutrophil elastase subverts the immune response by cleaving toll‐like receptors and cytokines in pneumococcal pneumonia. Frontiers in Immunology 9, 732.
    doi: 10.3389/fimmu.2018.00732google scholar: lookup
  6. Figueiredo M D, Salter C E, Andrietti A L P, Vandenplas M L, Hurley D J, Moore J N. Validation of a reliable set of primer pairs for measuring gene expression by real‐time quantitative RT‐PCR in equine leukocytes. Veterinary Immunology and Immunopathology 131(1–2), 65–72.
    doi: 10.1016/j.vetimm.2009.03.013google scholar: lookup
  7. Hoffmann C, Ellenberger C, Mattos R C, Aupperle H, Dhein S, Stief B, Schoon H.‐A.. The equine endometrosis: New insights into the pathogenesis. Animal Reproduction Science 111(2–4), 261–278.
    doi: 10.1016/j.anireprosci.2008.03.019google scholar: lookup
  8. Huebener P, Schwabe R F. Regulation of wound healing and organ fibrosis by toll‐like receptors. Biochimica et Biophysica Acta (BBA) – Molecular Basis of Disease 1832(7), 1005–1017.
    doi: 10.1016/j.bbadis.2012.11.017google scholar: lookup
  9. Kannaki T R, Shanmugam M, Verma P C. Toll‐like receptors and their role in animal reproduction. Animal Reproduction Science 125(1–4), 1–12.
    doi: 10.1016/j.anireprosci.2011.03.008google scholar: lookup
  10. Karampitsakos T, Woolard T, Bouros D, Tzouvelekis A. Toll‐like receptors in the pathogenesis of pulmonary fibrosis. European Journal of Pharmacology 808, 35–43.
    doi: 10.1016/j.ejphar.2016.06.045google scholar: lookup
  11. Kenney R M, Doig P A. Equine endometrial biopsy. In D. A. Morrow (Ed.), Current therapy in theriogenology 2: Diagnosis, treatment, and prevention of reproductive diseases in small and large animals (2nd ed., pp. 723–729).
  12. Pinto‐Bravo P, Ferreira‐Dias G, Rebordão M R, Amaral A, Fernandes C, Cuello C, Parrilla I, Martínez E, Roberto Da Costa R P, Skarzynski D J. Is mare endometrosis linked to oviduct fibrosis?. Pferdeheilkunde Equine Medicine 34(1), 43–46.
    doi: 10.21836/pem20180107google scholar: lookup
  13. Rebordão M R, Amaral A, Fernandes C, Silva E, Lukasik K, Szóstek‐Mioduchowska A, Pinto‐Bravo P, Galvão A, Skarzynski D J, Ferreira‐Dias G. Enzymes present in neutrophil extracellular traps may stimulate the fibrogenic pgf2α pathway in the mare endometrium. Animals 11(9), 2615.
    doi: 10.3390/ani11092615google scholar: lookup
  14. Rebordão M R, Amaral A, Lukasik K, Szóstek‐Mioduchowska A, Pinto‐Bravo P, Galvão A, Skarzynski D J, Ferreira‐Dias G. Constituents of neutrophil extracellular traps induce in vitro collagen formation in mare endometrium. Theriogenology 113, 8–18.
    doi: 10.1016/j.theriogenology.2018.02.001google scholar: lookup
  15. Rebordão M R, Amaral A, Lukasik K, Szóstek‐Mioduchowska A, Pinto‐Bravo P, Galvão A, Skarzynski D J, Ferreira‐Dias G. Impairment of the antifibrotic prostaglandin E2 pathway may influence neutrophil extracellular traps–induced fibrosis in the mare endometrium. Domestic Animal Endocrinology 67, 1–10.
    doi: 10.1016/j.domaniend.2018.10.004google scholar: lookup
  16. Roberto Da Costa R P, Serrão P M, Monteiro S, Pessa P, Silva J R, Ferreira‐Dias G. Caspase‐3‐mediated apoptosis and cell proliferation in the equine endometrium during the oestrous cycle. Reproduction, Fertility, and Development 19(8), 925–932.
    doi: 10.1071/rd06159google scholar: lookup
  17. Schöniger S, Gräfe H, Schoon H.‐A.. Expression of toll‐like receptors 2, 4 and 6 in different cell populations of the equine endometrium. Veterinary Immunology and Immunopathology 185, 7–13.
    doi: 10.1016/j.vetimm.2017.01.002google scholar: lookup
  18. Schwarz R I. Collagen I and the fibroblast: High protein expression requires a new paradigm of post‐transcriptional, feedback regulation. Biochemistry and Biophysics Reports 3, 38–44.
    doi: 10.1016/j.bbrep.2015.07.007google scholar: lookup
  19. Siemieniuch M J, Szóstek A Z, Gajos K, Kozdrowski R, Nowak M, Okuda K. Type of inflammation differentially affects expression of interleukin 1β and 6, tumor necrosis factor‐α and toll‐like receptors in subclinical endometritis in mares. PLoS One 11(5), e0154934.
    doi: 10.1371/journal.pone.0154934google scholar: lookup
  20. Szóstek‐Mioduchowska A Z, Baclawska A, Okuda K, Skarzynski D J. Effect of proinflammatory cytokines on endometrial collagen and metallopeptidase expression during the course of equine endometrosis. Cytokine 123, 154767.
    doi: 10.1016/j.cyto.2019.154767google scholar: lookup
  21. Szóstek‐Mioduchowska A Z, Lukasik K, Skarzynski D J, Okuda K. Effect of transforming growth factor‐β1 on α‐smooth muscle Actin and collagen expression in equine endometrial fibroblasts. Theriogenology 124, 9–17.
    doi: 10.1016/j.theriogenology.2018.10.005google scholar: lookup
  22. Vogel C, Marcotte E M. Insights into the regulation of protein abundance from proteomic and transcriptomic analyses. Nature Reviews Genetics 13(4), 227–232.
    doi: 10.1038/nrg3185google scholar: lookup

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