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Home / Equine Research Bank / J Vet Emerg Crit Care (San Antonio) / Use of FreeStyle Libre for continuous glucose monitoring in ...
Journal of veterinary emergency and critical care (San Antonio, Tex. : 2001)2024; doi: 10.1111/vec.13367

Use of FreeStyle Libre for continuous glucose monitoring in adult horses.

Abstract: To evaluate the feasibility of using the FreeStyle Libre (a continuous glucose monitoring system [CGMS]) for instantaneous continuous monitoring of interstitial glucose in adult horses and examine the applicability and accuracy of this system in horses submitted to combined glucose-insulin test (CGIT). Methods: Laboratory measurements and continuous glucose monitoring system (CGMS) readings were analyzed using a 2 × 2 factorial statistical model with repeated measures over time. This analysis assessed the effects of the test (factor 1), group (factor 2), and their interactions (test × group, test × time, and group × time). Pearson's correlation analysis was applied to blood glucose values. Mean comparisons were conducted using the t-test, and agreement between techniques was assessed via the Bland-Altman method, with a 95% confidence interval. Methods: Field study on private horse farms in association with a veterinary school. Methods: Ten healthy stallions were assigned to one of two groups based on their body condition scores (BCS). Group 1 (G1, n = 5) consisted of nonobese horses with a BCS of 5 or 6, while Group 2 (G2, n = 5) consisted of obese horses with a BCS of 7 or higher. Methods: A CGMS sensor was attached to the dorsolateral aspect of the proximal one third of each horse's neck. Laboratory blood glucose measurements and CGMS interstitial glucose readings were compared at different time points for up to 7 days after sensor fixation. Obese horses were also submitted to CGIT on Day 4. Results: A comparative analysis of glucose measurements obtained in G1 and G2 horses using the CGMS and enzymatic methods revealed significant group × time interactions (P < 0.001) and time effects (P < 0.001). No interactions were detected between group (P = 0.45), test (P = 0.62), group and test (P = 0.28), or time and test (P = 0.92). In G1 and G2, tests were significantly correlated (r = 0.84 and P = 0.00) at all time points (T0-T5). Agreement between the glucose values obtained using different methods was excellent despite a small time delay in CGMS detection of rapid changes in blood glucose. Conclusions: It was concluded that the CGMS can be used for indirect assessment of glycemic status (ie, based on interstitial glucose measurements) in nonobese and obese adult horses submitted to CGIT.
© Veterinary Emergency and Critical Care Society 2024.
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Publication Date: 2024-03-08 PubMed ID: 38459745DOI: 10.1111/vec.13367Google Scholar: Lookup
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Summary

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The research investigates the feasibility and accuracy of using the FreeStyle Libre, a continuous glucose monitoring system (CGMS), to monitor glucose levels in horses. This study also examines the system’s applicability in horses undergoing combined glucose-insulin tests.

Methodology

  • The study was conducted on ten healthy stallions from private horse farms. These horses were separated into two groups based on their body condition scores (BCS): one group of non-obese horses (Group 1) with a BCS between 5 and 6, and another group of obese horses (Group 2) with a BCS of 7 or higher.
  • The CGMS sensor was affixed to the upper part of each horse’s neck. For up to 7 days after the fixation of the sensor, blood glucose levels from laboratory measurements were compared with interstitial glucose readouts from the CGMS at different points in time. On the fourth day, the horses in Group 2 also underwent a combined glucose-insulin test (CGIT).

Statistical Analysis

  • The researchers used a 2 × 2 factorial statistical model to analyze the laboratory measurements and CGMS readings. This model evaluated the effects of the test, group, and their various interactions.
  • There was an application of Pearson’s correlation analysis to blood glucose values and a comparison of means conducted with the t-test. The agreement between the methods was assessed using the Bland-Altman method, and a 95% confidence interval was achieved.

Results

  • Analysis of glucose measurements from both groups of horses using the CGMS and enzyme methods showed significant interactions between the group and time, along with significant effects over time.
  • No significant interaction was found between the group, test, the group and the test, or time and test. The comparison tests in both groups showed a significant correlation at all-time points, despite a small delay in the CGMS’s detection of rapid changes in blood glucose.

Conclusions

  • The study concludes that the FreeStyle Libre CGMS can be effectively used for indirect glycemic status assessment in both non-obese and obese adult horses undergoing a CGIT. This assessment is based on interstitial glucose measurements.

Cite This Article

APA
Françoso R, Baccarin RY, de Siqueira RF, Belli CB. (2024). Use of FreeStyle Libre for continuous glucose monitoring in adult horses. J Vet Emerg Crit Care (San Antonio). https://doi.org/10.1111/vec.13367

Publication

Journal of veterinary emergency and critical care (San Antonio, Tex. : 2001)
ISSN: 1476-4431
NlmUniqueID: 101152804
Country: United States
Language: English

Researcher Affiliations

Françoso, Rafael
  • Centro de Ciências Agrárias, Universidade Estadual da Região Tocantins do Maranhão, Imperatriz, Brazil.
Baccarin, Raquel Y
  • Departamento de Clínica Médica, Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo, São Paulo, Brazil.
de Siqueira, Renata F
  • Departamento de Clínica de Grandes Animais, Centro de Ciências Rurais da Universidade Federal de Santa Maria, Santa Maria, Brazil.
Belli, Carla B
  • Departamento de Clínica Médica, Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo, São Paulo, Brazil.

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