Validation of a commercially available photometric analytical system for assessment of plasma oxidative status in healthy horses.
Abstract: Reactive oxygen species (ROS) are the end-products of physiologic functions in health. Oxidative stress occurs when endogenous antioxidants are insufficient to neutralize ROS in the system. As a result, ROS can damage DNA, RNA, proteins, lipids, and cell organelles. To obtain accurate measurements of plasma oxidative stress, levels of both oxidants and antioxidants must be measured. This study validates a commercially available, semi-quantitative, photometric analytical system that measures systemic determinants of reactive oxygen metabolites (dROM) and plasma antioxidant capacity (PAC) in stored equine plasma. The objectives of this work were: 1) to validate a photometric analytical system to quantify dROM and PAC in equine plasma; and 2) to determine expected results for these tests in healthy adult horses. We hypothesized that this system would reliably and reproducibly assess dROM and PAC in equine plasma. We observed expected, dose-dependent increases in dROM generated by adding increasing concentrations of HO or ascorbic acid to equine plasma to provide samples containing a known quantity of oxidants or antioxidants respectively. Mean dROM value in healthy horses was 103.3 ±20.7 U. Carr and mean PAC was 2881.0 ± 313.9 U. Cor. This system reliably and reproducibly quantified dROM and PAC in equine plasma samples.
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Publication Date: 2023-10-30 PubMed ID: 37952346DOI: 10.1016/j.vetimm.2023.110665Google Scholar: Lookup
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- Journal Article
Summary
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The research study investigated and validated the use of a photometric analytical system to accurately measure oxidative stress levels in the plasma of healthy horses. The research concluded that the commercially marketed system can reliably and repetitively measure determinants of reactive oxygen metabolites and plasma antioxidant capacity in equine plasma.
Overview of Oxidative Stress and Its Measurement
- The research focuses on understanding oxidative stress, which occurs when there is an imbalance between reactive oxygen species (ROS) and antioxidants in the body. Under normal physiological conditions, ROS are neutralized by internal antioxidants. However, when antioxidants are insufficient, ROS can cause damage to cellular components like DNA, RNA, proteins, and lipids.
- To precisely assess oxidative stress, it’s essential to measure not just the oxidants but also the antioxidant levels in the system.
- Therefore, having an accurate, reliable method to evaluate both elements is crucial, and this study focused on testing such a system.
About The Photometric Analytical System
- The researchers validated a commercially available, semi-quantitative, photometric analytical system that measures systemic determinants of reactive oxygen metabolites (dROM) and plasma antioxidant capacity (PAC).
- This system was used on stored equine plasma, with the research’s goal to validate its use in quantifying dROM and PAC, and to establish expected results for these tests in healthy horses.
Experiments and Results
- The researchers created controlled equine plasma samples by adding varying concentrations of HO or ascorbic acid to mimic known quantities of oxidants or antioxidants, respectively.
- As they increased the amounts of these substances, they observed expected, dose-dependent increases in dROM, confirming that the system could accurately measure these changes.
- They found the mean dROM value in healthy horses to be 103.3 ±20.7 U. Carr and mean PAC at 2881.0 ± 313.9 U. Cor.
Conclusion
- Based on their results, the researchers concluded that the photometric analytical system can reliably and reproducibly assess dROM and PAC in equine plasma.
- This system thus offers a robust and verified way of evaluating and monitoring the oxidative stress status in horses, thereby aiding in early detection and treatment of potential health conditions.
Cite This Article
APA
Vaughn SA, Norton NA, Hurley DJ, Hart KA.
(2023).
Validation of a commercially available photometric analytical system for assessment of plasma oxidative status in healthy horses.
Vet Immunol Immunopathol, 265, 110665.
https://doi.org/10.1016/j.vetimm.2023.110665 Publication
Researcher Affiliations
- Department of Large Animal Medicine, University of Georgia College of Veterinary Medicine, 2200 College Station Road, Athens, GA 30602, USA.
- Department of Large Animal Medicine, University of Georgia College of Veterinary Medicine, 2200 College Station Road, Athens, GA 30602, USA.
- Department of Population Health, University of Georgia College of Veterinary Medicine, 2200 College Station Road, Athens, GA 30602, USA.
- Department of Large Animal Medicine, University of Georgia College of Veterinary Medicine, 2200 College Station Road, Athens, GA 30602, USA. Electronic address: khart4@uga.edu.
MeSH Terms
- Animals
- Horses
- Reactive Oxygen Species / metabolism
- Hydrogen Peroxide
- Antioxidants / metabolism
- Oxidative Stress
- Oxidants
Conflict of Interest Statement
Declaration of Competing Interest None.
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