FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / Experientia / Vitamins and other metabolites in various sera commonly used...
Experientia1988; 44(11-12); 1007-1010; doi: 10.1007/BF01939904

Vitamins and other metabolites in various sera commonly used for cell culturing.

Abstract: Many cell culture media use different sera to enhance growth. We assayed vitamins and some related metabolites in different sera and identified the concentration of: thiamin, biotin, folates, riboflavin, pantothenates, nicotinates, vitamins B6, B12, A, E, C, and carotenes and some related metabolites: biopterins, free inositol, free and total choline, total carnitines in chicken, horse, rabbit, goat, pig, calf, newborn calf, fetal calf and human sera. Results indicate that vitamin and metabolite content of different sera vary. Such variations could produce fluctuant effects on cell culturings if the metabolite content of the serum is not documented.
Get Full Text
Publication Date: 1988-12-01 PubMed ID: 3197804DOI: 10.1007/BF01939904Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Comparative Study
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article investigates the variations in vitamins and related metabolites in different types of sera commonly used in cell culturing and highlights their potential impact on the results of cell culturing if these differences are not properly documented.

Research Methodology

  • The researchers assayed various vitamins and related metabolites in different sera that are commonly used in culture media for the growth of cells. The different sera types assayed included chicken, horse, rabbit, goat, pig, calf, newborn calf, fetal calf, and human sera.
  • The vitamins researched included common vitamins like A, C, E, B6, B12, along with thiamin, biotin, folates, riboflavin, pantothenates, nicotinates, and carotenes. The related metabolites investigated included biopterins, free inositol, free and total choline, and total carnitines.

Key Findings

  • Results from the study showed that the concentration of vitamins and metabolites varied significantly across the different types of sera. This means that the vitamin and metabolite content of different sera, such as chicken, horse, rabbit, goat, pig, calf, newborn calf, fetal calf, and human sera can differ.
  • The researchers highlighted that these variations could have fluctuating effects on cell culture results, suggesting that the outcome of cell cultures could change depending on the type of serum used due to the different vitamin and metabolite contents.

Implications

  • According to the findings of this study, not accounting for the potential variations in vitamins and metabolites in different sera could lead to inaccuracies or inconsistencies in cell culturing. It therefore stresses the need for the vitamin and metabolite content of the serum to be properly documented before it’s used for cell culturing.
  • These results could provide valuable insights for scientists and researchers who perform cell culturing, helping them to select the most suitable serum and ensure the consistency and accuracy of their results.

Cite This Article

APA
Baker H, DeAngelis B, Frank O. (1988). Vitamins and other metabolites in various sera commonly used for cell culturing. Experientia, 44(11-12), 1007-1010. https://doi.org/10.1007/BF01939904

Publication

Experientia
ISSN: 0014-4754
NlmUniqueID: 0376547
Country: Switzerland
Language: English
Volume: 44
Issue: 11-12
Pages: 1007-1010

Researcher Affiliations

Baker, H
  • New Jersey Medical School, Department of Preventive Medicine and Community Health, Newark 07107.
DeAngelis, B
    Frank, O

      MeSH Terms

      • Animals
      • Biopterin / blood
      • Blood
      • Carnitine / blood
      • Cattle
      • Cells, Cultured
      • Chickens / blood
      • Choline / blood
      • Culture Media
      • Fetal Blood
      • Goats / blood
      • Horses / blood
      • Humans
      • Inositol / blood
      • Rabbits
      • Swine / blood
      • Vitamins / blood

      References

      This article includes 12 references
      1. BAILEY JM. LIPID METABOLISM IN CULTURED CELLS. V. COMPARATIVE LIPID NUTRITION IN SERUM AND IN LIPID-FREE CHEMICALLY DEFINED MEDIUM.. Proc Soc Exp Biol Med 1964 Mar;115:747-50.
        pubmed: 14155817doi: 10.3181/00379727-115-29026google scholar: lookup
      2. Healy GM, Parker RC. Cultivation of mammalian cells in defined media with protein and nonprotein supplements.. J Cell Biol 1966 Sep;30(3):539-53.
        pubmed: 5971006doi: 10.1083/jcb.30.3.539google scholar: lookup
      3. TOPLIN I. Experiences with the tissue culture system in large-scale cancer chemotherapy screening.. Cancer Res 1961 Sep;21:1042-6.
        pubmed: 13777450
      4. Baker H, Frank O, Tuma DJ, Barak AJ, Sorrell MF, Hutner SH. Assay for free and total choline activity in biological fluids and tissues of rats and man with Torulopsis pintolopessi.. Am J Clin Nutr 1978 Mar;31(3):532-40.
        pubmed: 564602doi: 10.1093/ajcn/31.3.532google scholar: lookup
      5. Baker H, Frank O, Bacchi CJ, Hunter SH. Biopterin content of human and rat fluids and tissues determined protozoologically.. Am J Clin Nutr 1974 Nov;27(11):1247-53.
        pubmed: 4447093doi: 10.1093/ajcn/27.11.1247google scholar: lookup
      6. Weiler JM, Ballas ZK, Feldbush TL, Needleman BW. Inhibition of human lymphocyte blastogenesis by C3: the role of serum in the tissue culture medium.. Immunology 1982 Jun;46(2):247-52.
        pubmed: 6979506
      7. Baker H, Schor SM, Murphy BD, DeAngelis B, Feingold S, Frank O. Blood vitamin and choline concentrations in healthy domestic cats, dogs, and horses.. Am J Vet Res 1986 Jul;47(7):1468-71.
        pubmed: 3740617
      8. Daniel MR, Dingle JT, Glauert AM, Lucy JA. The action of excess of vitamin A alcohol on the fine structure of rat dermal fibroblasts.. J Cell Biol 1966 Sep;30(3):465-75.
        pubmed: 5971002doi: 10.1083/jcb.30.3.465google scholar: lookup
      9. De Luca C, Carruthers C, Tritsch GL. Extracts of plasma and serum toxic for mammalian cells cultured in vitro.. Exp Cell Res 1966 Sep;43(2):451-8.
        pubmed: 4162160doi: 10.1016/0014-4827(66)90072-3google scholar: lookup
      10. MOHBERG J, JOHNSON MJ. STABILITY OF VITAMINS IN A CHEMICALLY DEFINED MEDIUM FOR 929-L FIBROBLASTS.. J Natl Cancer Inst 1963 Sep;31:603-10.
        pubmed: 14059005
      11. Rebouche CJ, Paulson DJ. Carnitine metabolism and function in humans.. Annu Rev Nutr 1986;6:41-66.
        pubmed: 3524622doi: 10.1146/annurev.nu.06.070186.000353google scholar: lookup
      12. HOLMES R. Long-term cultivation of human cells (Chang) in chemically defined medium and effect of added peptone fractions.. J Biophys Biochem Cytol 1959 Dec;6(3):535-6.
        pubmed: 14402792doi: 10.1083/jcb.6.3.535google scholar: lookup

      Citations

      This article has been cited 12 times.
      1. Wang M, Zhao K, Liu M, Wang M, Qiao Z, Yi S, Jiang Y, Kou X, Zhao Y, Yin J, Li T, Wang H, Jiang C, Gao S, Chen J. BMP4 preserves the developmental potential of mESCs through Ube2s- and Chmp4b-mediated chromosomal stability safeguarding.. Protein Cell 2022 Aug;13(8):580-601.
        doi: 10.1007/s13238-021-00896-xpubmed: 35147915google scholar: lookup
      2. Punia S, Juran BD, Ali AH, Schlicht EM, Moore RM, Sun Z, Lazaridis KN. Evaluation of circulating cell-free DNA in cholestatic liver disease using liver-specific methylation markers.. BMC Gastroenterol 2021 Apr 1;21(1):149.
        doi: 10.1186/s12876-021-01741-5pubmed: 33794792google scholar: lookup
      3. Karadjian M, Senger AS, Essers C, Wilkesmann S, Heller R, Fellenberg J, Simon R, Westhauser F. Human Platelet Lysate Can Replace Fetal Calf Serum as a Protein Source to Promote Expansion and Osteogenic Differentiation of Human Bone-Marrow-Derived Mesenchymal Stromal Cells.. Cells 2020 Apr 9;9(4).
        doi: 10.3390/cells9040918pubmed: 32283663google scholar: lookup
      4. Jones DC, Alphey MS, Wyllie S, Fairlamb AH. Chemical, genetic and structural assessment of pyridoxal kinase as a drug target in the African trypanosome.. Mol Microbiol 2012 Oct;86(1):51-64.
        doi: 10.1111/j.1365-2958.2012.08189.xpubmed: 22857512google scholar: lookup
      5. Coustou V, Guegan F, Plazolles N, Baltz T. Complete in vitro life cycle of Trypanosoma congolense: development of genetic tools.. PLoS Negl Trop Dis 2010 Mar 2;4(3):e618.
        doi: 10.1371/journal.pntd.0000618pubmed: 20209144google scholar: lookup
      6. Shi L, Ansong C, Smallwood H, Rommereim L, McDermott JE, Brewer HM, Norbeck AD, Taylor RC, Gustin JK, Heffron F, Smith RD, Adkins JN. Proteome of Salmonella Enterica Serotype Typhimurium Grown in a Low Mg/pH Medium.. J Proteomics Bioinform 2009;2:388-397.
        doi: 10.4172/jpb.1000099pubmed: 19953200google scholar: lookup
      7. Nanda PK, Swain P, Nayak SK, Dash S, Routray P, Swain SK, Patra BC. Goat serum as an alternative to establish cell culture from Indian major carp, Cirrhinus mrigala.. In Vitro Cell Dev Biol Anim 2009 Mar-Apr;45(3-4):148-51.
        doi: 10.1007/s11626-008-9160-7pubmed: 19118441google scholar: lookup
      8. Ducommun P, Ruffieux PA, von Stockar U, Marison I. The role of vitamins and amino acids on hybridoma growth and monoclonal antibody production.. Cytotechnology 2001 Oct;37(2):65-73.
        doi: 10.1023/A:1019956013627pubmed: 19002903google scholar: lookup
      9. Park YK, Hwang MK, Jung YJ. Complement-mediated tail degradation of Neodiplostomum seoulense cercariae.. Korean J Parasitol 2006 Jun;44(2):127-31.
        doi: 10.3347/kjp.2006.44.2.127pubmed: 16809960google scholar: lookup
      10. Lott WB, Takyar SS, Tuppen J, Crawford DH, Harrison M, Sloots TP, Gowans EJ. Vitamin B12 and hepatitis C: molecular biology and human pathology.. Proc Natl Acad Sci U S A 2001 Apr 24;98(9):4916-21.
        doi: 10.1073/pnas.081072798pubmed: 11296247google scholar: lookup
      11. Wilson JX, Jaworski EM, Kulaga A, Dixon SJ. Substrate regulation of ascorbate transport activity in astrocytes.. Neurochem Res 1990 Oct;15(10):1037-43.
        doi: 10.1007/BF00965751pubmed: 2077429google scholar: lookup
      12. Wyss C. Growth of Porphyromonas gingivalis, Treponema denticola, T. pectinovorum, T. socranskii, and T. vincentii in a chemically defined medium.. J Clin Microbiol 1992 Sep;30(9):2225-9.
        doi: 10.1128/jcm.30.9.2225-2229.1992pubmed: 1400984google scholar: lookup
      Subscribe to our newsletter
      Join 99,344 horse owners like you who receive our email updates on horse health and nutrition.