Antibodies.
Publisher:
MDPI AG,
Frequency: Quarterly
Country: Switzerland
Language: English
Start Year:2012 -
ISSN:
2073-4468 (Electronic)
2073-4468 (Linking)
2073-4468 (Linking)
Impact Factor
4.7
2022
| NLM ID: | 101587489 |
| (OCoLC): | 820898931 |
| Coden: | ANTICA |
| LCCN: | 2013247510 |
Binding Analysis of Human Immunoglobulin G as a Zinc-Binding Protein. Human immunoglobulin G (IgG) binding with zinc ions was examined using zinc ions immobilized on chelating Sepharose beads (Zn-beads). Human IgG bound to Zn-beads but not to Sepharose beads (control beads). Mouse, rat, bovine and equine IgGs also bound to Zn-beads, similar to human IgG. The human IgG F(c) fragment showed zinc ion-binding activity whereas the Fab fragment did not. Ethylenediaminetetraacetic acid (EDTA)-treated Zn-beads no longer bound human IgG; however, washing the beads, followed by the addition of zinc ions, restored the binding activity towards human IgG. Zn-beads saturated ...