Biology of the cell
Publisher:
Publié par la Société française de microscopie électronique avec le concours du Centre national de la recherche scientifique et de l'Institute national de la santé et de la recherche médicale,. Chichester : Wiley-Blackwell (2012)
Frequency: Monthly, 2005-
Country: England
Language: English
Author(s):
Société française de microscopie électronique., European Cell Biology Organization., Centre national de la recherche scientifique (France), Institut national de la santé et de la recherche médicale (France), Société française des microscopies., Société de biologie cellulaire de France.
Start Year:1981 -
ISSN:
0248-4900 (Print)
1768-322X (Electronic)
0248-4900 (Linking)
1768-322X (Electronic)
0248-4900 (Linking)
Impact Factor
2.7
2022
| NLM ID: | 8108529 |
| (DNLM): | B17725000(s) |
| (OCoLC): | 07381466 |
| Coden: | BCELDF |
| Classification: | W1 BI852NL |
Lysosomal arylsulfatases A and B from horse blood leukocytes: purification and physico-chemical properties. Lysosomal arylsulfatases A and B (aryl-sulfate sulfohydrolases, EC 3.1.6.1) from horse leukocytes were purified about 680-fold and 70-fold, respectively, starting from a crude extract of the azurophil and specific granules of leukocytes, by affinity, ion exchange, and gel filtration chromatography. Purified arylsulfatase A displayed anomalous kinetics, a pH optimum at 5.2, an isoelectric point at 4.3, and a Km value for p-nitrocatechol sulfate (pNCS) of 0.37 mM. This enzyme was found to exist in two association states depending on pH: a high molecular weight form at pH 5.0 and a low molecular ...