Chemistry and physics of lipids.
Publisher:
Elsevier Science Ireland Ltd. Limerick : Elsevier Science Ireland Ltd
Frequency: Twelve no. a year, 2013-
Country: Ireland
Language: English
Start Year:1966 -
ISSN:
0009-3084 (Print)
1873-2941 (Electronic)
0009-3084 (Linking)
1873-2941 (Electronic)
0009-3084 (Linking)
Impact Factor
3.4
2022
| NLM ID: | 0067206 |
| (DNLM): | C16700000(s) |
| (OCoLC): | 00876631 |
| Coden: | CPLIA4 |
| LCCN: | sn 80003045 |
| Classification: | W1 CH36E |
A fast method for the determination of the PC/LPC ratio in intact serum by MALDI-TOF MS: an easy-to-follow lipid biomarker of inflammation. The PC/LPC ratio of blood serum is increasingly considered to represent an important clinical parameter that reflects various kinds of diseases. Here, a simple and fast method of lipid analyses of "intact" blood serum (i.e. without extraction) by MALDI-TOF mass spectrometry is described. The novel procedure allows the accurate determination of the PC/LPC ratio, utilizing only a tiny amount of blood. The serum is diluted with distilled water and directly applied onto the MALDI target and, after drying, covered by a thin layer of the matrix solution (either 9-aminoacridine or 2,5-dihydroxybenzoi...