Conservation genetics resources.
Publisher:
Springer
Country: Netherlands
Language: English
Start Year:2009 -
Identifiers
| ISSN: | 1877-7252 (Print) 1877-7260 (Electronic) |
| NLM ID: | 101505405 |
| (OCoLC): | 885843770 |
Targeted genome-wide SNP genotyping in feral horses using non-invasive fecal swabs. The development of high-throughput sequencing has prompted a transition in wildlife genetics from using microsatellites toward sets of single nucleotide polymorphisms (SNPs). However, genotyping large numbers of targeted SNPs using non-invasive samples remains challenging due to relatively large DNA input requirements. Recently, target enrichment has emerged as a promising approach requiring little template DNA. We assessed the efficacy of Tecan Genomics' Allegro Targeted Genotyping (ATG) for generating genome-wide SNP data in feral horses using DNA isolated from fecal swabs. Total and host-sp...