Current research in pharmacology and drug discovery.
Publisher:
Elsevier B.V.,
Country: Netherlands
Language: English
Start Year:2020 -
Identifiers
| ISSN: | 2590-2571 (Electronic) 2590-2571 (Linking) |
| NLM ID: | 97733 |
| (OCoLC): | 1113324417 |
| LCCN: | 2021204376 |
| Classification: | W1 |
Bupivacaine in combination with sildenafil (Viagra) and vitamin D3 have anti-inflammatory effects in osteoarthritic chondrocytes. To treat osteoarthritic chondrocytes and thereby reduce the inflammation with a drug combination that primarily affects 5-HT- and ATP-evoked Ca2+ signaling. In osteoarthritic chondrocytes, Ca2+ signaling is elevated, resulting in increased production of ATP and inflammatory mediators. The expression of TLR4 and Na+/K+-ATPase was used to evaluate the inflammatory status of the cells. Methods: Equine chondrocytes were collected from joints with mild structural osteoarthritic changes and cultured in monolayers. The cells were treated with a combination of bupivacaine (1 pM) and sildenafil (1 ...