The Journal of general physiology.
Publisher:
Rockefeller Institute for Medical Research,. New York, N.Y. : Rockefeller University Press
Frequency: Monthly
Country: United States
Language: English
Author(s):
Rockefeller Institute for Medical Research., Rockefeller Institute, Society of General Physiologists.
Start Year:1918 -
ISSN:
0022-1295 (Print)
1540-7748 (Electronic)
0022-1295 (Linking)
1540-7748 (Electronic)
0022-1295 (Linking)
Impact Factor
3.7
| NLM ID: | 257978 |
| (DNLM): | J21980000(s) |
| (OCoLC): | 01390169 |
| Coden: | JGPLAD |
| Classification: | W1 JO668C |
Redox regulation of large conductance Ca(2+)-activated K+ channels in smooth muscle cells. The effects of sulfhydryl reduction/oxidation on the gating of large-conductance, Ca(2+)-activated K+ (maxi-K) channels were examined in excised patches from tracheal myocytes. Channel activity was modified by sulfhydryl redox agents applied to the cytosolic surface, but not the extracellular surface, of membrane patches. Sulfhydryl reducing agents dithiothreitol, beta-mercaptoethanol, and GSH augmented, whereas sulfhydryl oxidizing agents diamide, thimerosal, and 2,2'-dithiodipyridine inhibited, channel activity in a concentration-dependent manner. Channel stimulation by reduction and inhibit...
Cell and Solution Velocity Constants for the Reaction CO + Hb –> COHb at Different Temperatures in Mammals with Different Red Cell Sizes. Using a double beam stopped-flow apparatus, measurements were made of the velocity constant of the reaction CO + Hb --> COHb in solution and in the red cells of human beings, rabbits, horses, and goats. The solution constant (l') at 37 degrees C for human beings was 362 mM(-1) sec.(-1); in other species l' was somewhat lower. Two rabbits, despite having apparently identical hemoglobins had significantly different values for l'. The energy of activation (E) of l' was between 8 and 11 kcal/mole in all cases. The cell reaction constant (l'(c)) at 37 degrees was between 61 and 73 mM(-1) sec.(-1...
Some aspects of the desoxyribonuclease activities of animal tissues. It has been found that many animal tissues contain "acid" desoxyribonucleases with pH optima near 5.2. A chemical method for the determination of this activity is described. The pancreatic desoxyribonuclease crystallized by Kunitz and shown to have a neutral pH optimum occurs in the pancreas together with the "acid" enzyme, but only the "neutral" enzyme occurs in the pancreatic juice. The ratio of "neutral" to "acid" DNAase activities in the pancreas is greater than 200, but in all other tissues examined there is no appreciable concentration of the neutral enzyme. It is concluded that neutral ...