Journal of molecular and genetic medicine : an international journal of biomedical research.
Publisher:
Library Pub. Media,. Los Angeles, CA : OMICS Publishing Group (2014)
Frequency: Bimonthly
Country: England
Language: English
Start Year:2005 -
ISSN:
1747-0862 (Electronic)
1747-0862 (Linking)
1747-0862 (Linking)
Impact Factor
4.4
2019
| NLM ID: | 101256516 |
| (OCoLC): | 61660528 |
| LCCN: | 2005243885 |
The use of equine influenza pseudotypes for serological screening. Standard assays used for influenza serology present certain practical issues, such as inter-laboratory variability, complex protocols and the necessity for handling certain virus strains in high biological containment facilities. In an attempt to address this, avian and human influenza HA pseudotyped retroviruses have been successfully employed in antibody neutralization assays. In this study we generated an equine influenza pseudotyped lentivirus for serological screening. This was achieved by co-transfection of HEK293T cells with plasmids expressing the haemagglutinin (HA) protein of an H3N8...