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Journal de pharmacologie.
Discontinued
Publisher:
Masson.. Paris : Masson Et Cie
Frequency: Quarterly
Country: France
Language: fre
Author(s):
Association des pharmacologistes (France)
Start Year:1970 - 1986
Identifiers
| ISSN: | 0021-793X (Print) 0021-793X (Linking) |
| NLM ID: | 1247760 |
| (DNLM): | J34560000(s) |
| (OCoLC): | 02262981 |
| Coden: | JNPHAG |
| Classification: | W1 JO328U |
Solubilization and characterization of [3H] 5HT high affinity binding sites (5HT1 and 5HT3). The solubilization of the serotonergic 5HT1 and 5HT3 sites was performed with digitonin and sodium cholate at 1% (final concentration). Two binding sites for [3H]5HT were observed on rat or horse brain synaptosomal membranes solubilized with these detergents. The corresponding dissociation constants (KD) were 1-3 nM and 13-30 nM respectively. These values were closely similar to those corresponding to 5HT1 and 5HT3 sites located in intact membranes. The solubilized sites specifically bound 5HT. The effect of GTP decreasing the binding to 5HT1 sites was lost on solubilized 5HT1 sites; it was re...
