Metabolic brain disease.
Publisher:
Plenum,. Amsterdam : Springer (2005)
Frequency: Quarterly
Country: United States
Language: English
Start Year:1986 -
ISSN:
0885-7490 (Print)
1573-7365 (Electronic)
0885-7490 (Linking)
1573-7365 (Electronic)
0885-7490 (Linking)
Impact Factor
3.6
2022
| NLM ID: | 8610370 |
| (DNLM): | SR0055799(s) |
| (OCoLC): | 12712938 |
| Coden: | MBDIEE |
| LCCN: | 89645534 |
| Classification: | W1 ME961LM |
Effect of serum on intracellular calcium homeostasis and survival of primary cortical and hippocampal CA1 neurons following brief glutamate treatment. Glutamate neurotoxicity was studied in primary neuronal cultures prepared from rat cerebral cortex and hippocampal CA1 sector. Neurons were cultivated with 5% native horse serum and then exposed to 0.1 or 1.0 mM glutamate for 5 min. Subsequently, neurons were allowed to recover for 24 hours either in the presence or in the absence of 5% native horse serum. In the absence of serum, neurons showed morphological signs of degeneration and exhibited marked loss of vitality as tested by vital staining and release of lactate dehydrogenase (LDH). In contrast, when neurons were cultivated in the presen...