The open microbiology journal.
Publisher:
Bentham Science Publishers,. [Sharjah, U.A.E] : Bentham Open (2009)
Frequency: Annual
Country: United Arab Emirates
Language: English
Start Year:2007 -
Identifiers
| ISSN: | 1874-2858 (Electronic) 1874-2858 (Linking) |
| NLM ID: | 101480482 |
| (OCoLC): | 226370260 |
| LCCN: | 2008263457 |
IgG Endopeptidase SeMac does not Inhibit Opsonophagocytosis of Streptococcus equi Subspecies equi by Horse Polymorphonuclear Leukocytes. The secreted Mac protein made by group A Streptococcus (GAS) inhibits opsonophagocytosis of GAS by human polymorphonuclear leukocytes (PMNs). This protein also has the endopeptidase activity against human immunoglobulin G (IgG), and the Cys94, His262 and Asp284 are critical for the enzymatic activity. The horse pathogen Streptococcus equi subspecies equi produces a homologue of Mac (SeMac). SeMac was characterized to determine whether SeMac has IgG endopeptidase activity and inhibits opsonophagocytosis of S. equi by horse PMNs. The gene was cloned and recombinant SeMac was overexpressed in Esc...