Patologicheskaia fiziologiia i èksperimental'naia terapiia.
Publisher:
Izdatelstvo Meditsina
Frequency: Four no. a year
Country: Russia (Federation)
Language: Russian
Author(s):
Soviet Union. Ministerstvo zdravookhranenii︠a︡
Start Year:1957 -
Identifiers
| ISSN: | 0031-2991 (Print) 0031-2991 (Linking) |
| NLM ID: | 0376421 |
| (DNLM): | P05500000(s) |
| (OCoLC): | 01681126 |
| Coden: | PAFEAY |
| Classification: | W1 PA988 |
Destabilase-lysozyme-2 – original recombinant thrombolytic preparation of medicinal leech inhibits horse platelets aggregation. The purpose. Identifying the capacity of the medicinal leech novel original recombinant thrombolytic preparation Destabilase-Lysozyme-2 to inhibit the blood platelet aggregation. Gene of destabilase-lysozyme. ds2 (mlDL-Ds2 ), was cloned in E.coli cells. Recombinant protein was isolated in denaturing conditions using metal-chelate chromatography followed by denaturation of the polypeptide by rapid dilution in exact accordance with the procedure described by Kurdyumov A.S. et al. ( 2016, Russian Journal of Bioorganic Chemistry, v.42, s. 42-52). Blood was collected from the jugular vein of 18 hor...