Prostaglandins, leukotrienes, and essential fatty acids.
Publisher:
Churchill Livingstone,
Frequency: Monthly
Country: Scotland
Language: English
Start Year:1988 -
ISSN:
0952-3278 (Print)
1532-2823 (Electronic)
0952-3278 (Linking)
1532-2823 (Electronic)
0952-3278 (Linking)
Impact Factor
3
2022
| NLM ID: | 8802730 |
| (DNLM): | SR0061715(s) |
| (OCoLC): | 17466156 |
| Coden: | PLEAEU |
| Classification: | W1 PR77TJ |
Optimization of assay conditions for leukotriene B4 synthesis by neutrophils or platelets isolated from peripheral blood of monogastric animals. Neutrophils are involved in inflammation through leukotriene (LT) production. The predominant proinflammatory leukotriene released from neutrophils is LTB4, which serves as a biological marker of inflammation. The purpose of this study was to optimize the conditions ex vivo for LTB4 production by neutrophils from horses and dogs, and platelets from chickens. Optimal production of LTB4 was characterized by incubation time (2.5, 5, 10, 15 or 20 min), temperature (25 or 37 degrees C), and calcium ionophore A23187 concentration (0.1, 1, 10 or 20 microM). Incubation longer than 2.5 min did not incr...
PGE2 inhibits acetylcholine release from cholinergic nerves in canine but not equine airways. The effects of exogenous prostaglandin E2 (PGE2) and endogenous prostanoids on cholinergic neurotransmission were determined by measurement of acetylcholine (ACh) release from canine and equine airway tissues. Trachealis strips and bronchial segments were suspended in 2 ml tissue baths. ACh release was induced by electrical field stimulation (EFS), and its content in tissue bath liquid was measured by high pressure liquid chromatography (HPLC) with electrochemical detection. In canine airways, exogenous PGE2 (10(-9) to 10(-7) M) inhibited ACh release concentration-dependently, whereas inhibiti...