[3H]ouabain binding in skeletal muscle from horses with hyperkalemic periodic paralysis.
Abstract: Ouabain, a cardiac glycoside, binds to the Na(+)-K(+)-adenosine triphosphatase (Na+ pump) and prevents active transport of Na+ and K+ across cell membranes. We used [3H]ouabain to quantify the number and affinity of Na+ pumps in skeletal muscle from Quarter Horses with the muscular disorder hyperkalemic periodic paralysis (HYPP). [3H]Ouabain-binding properties of gluteal muscle from clinically normal and affected horses were used to determine whether altered Na+ pump number or affinity could contribute to the pathologic features of muscle in affected horses. Foals and adult horses with HYPP were compared with age-matched clinically normal horses. The number of [3H]ouabain-binding sites in adult gluteal muscle was not different between the 2 types of horses (85.7 +/- 8.9 pmol of [3H]ouabain-binding sites/g [wet muscle weight] in horses with HYPP vs 100.2 +/- 8.8 pmol/g in clinically normal adult horses). Gluteal muscles in HYPP-affected and clinically normal foals also contained a similar number of [3H]ouabain-binding sites (222.3 +/- 21.0 pmol/g vs 225.3 +/- 24.2 pmol/g, respectively). The affinity of these binding sites for ouabain was not different, between adults or foals, in clinically normal or affected horses. Our results indicate that membrane events underlying the periodic episodes of paralysis in horses with HYPP are not attributable to quantitative changes in Na+ pump number or affinity. Our data cannot exclude the possibility that the specific activity of the Na+ pump is altered in muscle from HYPP-affected horses.
Publication Date: 1993-05-01 PubMed ID: 8391230
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article investigates whether changes in the number or functionality of sodium pumps in muscle cells might contribute to the muscle disorder known as hyperkalemic periodic paralysis (HYPP) in horses. Researchers used a substance known as ouabain, which binds to these sodium pumps, to test their hypothesis but found no significant difference in sodium pump numbers or performance in affected versus healthy horses, suggesting that this is not the source of the disorder.
Understanding the Research
- The goal of the research was to understand more about a muscular disease in horses named hyperkalemic periodic paralysis (HYPP) by analyzing the sodium (Na+) pumps in the skeletal muscle of affected horses and comparing them to those in healthy horses.
- The researchers used ouabain, a cardiac glycoside, which impeded the active transport of sodium and potassium across cell membranes by binding to the Na+ pump, as a tool to investigate the function and quantity of Na+ pumps present in skeletal muscle.
Research Process and Results
- The [3H]ouabain-binding properties of skeletal muscles (specifically the gluteal muscles) in both healthy and affected horses was studied, which allowed the researchers to quantify and compare the number and affinity of sodium pumps. This was done to assess whether there were any differences between the two that could be contributing to the disorder in affected horses.
- The number of the sodium pump sites that [3H]ouabain could bind to was not significantly different in adult horses or foals, regardless of whether they were affected by HYPP or were healthy.
- The affinity, or the degree to which the ouabain could bind to the sodium pumps, was also not significantly different between affected and healthy horses, regardless of their age (foals or adults).
Conclusions from the Findings
- These results indicate that a change in the number or affinity of sodium pumps in muscle cells of horses is not the reason for the periodic paralysis incidents associated with HYPP.
- This does not entirely rule out the sodium pump as a contributing factor to the disease. There’s still a possibility of altered specific activity of the sodium pump in the muscles of horses affected by HYPP, which the researchers did not exclude.
Cite This Article
APA
Pickar JG, Spier SJ, Harrold D, Carlsen RC.
(1993).
[3H]ouabain binding in skeletal muscle from horses with hyperkalemic periodic paralysis.
Am J Vet Res, 54(5), 783-787.
Publication
Researcher Affiliations
- Department of Human Physiology, School of Medicine, University of California, Davis 95616.
MeSH Terms
- Animals
- Binding Sites
- Horse Diseases / metabolism
- Horses
- Hyperkalemia / metabolism
- Hyperkalemia / veterinary
- Kinetics
- Muscles / metabolism
- Ouabain / metabolism
- Paralyses, Familial Periodic / metabolism
- Paralyses, Familial Periodic / veterinary
- Reference Values
- Sodium-Potassium-Exchanging ATPase / metabolism
Citations
This article has been cited 2 times.- Clausen T, Nielsen OB, Clausen JD, Pedersen TH, Hayward LJ. Na+,K+-pump stimulation improves contractility in isolated muscles of mice with hyperkalemic periodic paralysis. J Gen Physiol 2011 Jul;138(1):117-30.
- Veeneklaas RJ, Verkleij CB, van Schie B, Harun MA, Everts ME. Preliminary studies on the concentration of Na+,K(+)-ATPase in skeletal muscle of draught cattle in Mozambique: effect of sex, age and training. Trop Anim Health Prod 2002 Sep;34(5):431-47.
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