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Home / Equine Research Bank / FEMS Microbiol Ecol / A comparison of the microbiome and the metabolome of differe...
FEMS microbiology ecology2012; 82(3); 642-652; doi: 10.1111/j.1574-6941.2012.01441.x

A comparison of the microbiome and the metabolome of different regions of the equine hindgut.

Abstract: The microbiome and associated metabolome of faecal samples were compared to those from the caecum and right dorsal colon of horses and ponies euthanised for nonresearch purposes by investigating the microbial population community structure as well as their functional metabolic products. Through the use of 16S rRNA gene dendrograms, the caecum microbiome was shown to cluster separately from the other gut regions. 16S rRNA gene-based quantitative PCR (q-PCR) also demonstrated differences between the caecum and the other gut regions. Metabolites as identified by Fourier transform infrared clustered in a similar way and specific metabolic products (volatile fatty acids and ammonia) also varied by region. Protozoal 18S rDNA concentration and archaeal mcrA gene concentration quantified by q-PCR were found in higher numbers in the colon than the other gut regions. Diversity calculations using Simpson and Shannon-Wiener indices demonstrated higher diversity in the right dorsal colon and faeces than in the caecum. All findings of this study suggest that faecal samples are likely to represent the microbial population of the right dorsal colon to some extent but not that of the caecum, indicating careful consideration is required when planning microbial investigations of the hindgut of the horse.
© 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
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Publication Date: 2012-07-23 PubMed ID: 22757649DOI: 10.1111/j.1574-6941.2012.01441.xGoogle Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research paper studies the differences between the microbiome (the microorganisms in a particular environment) and metabolome (the complete set of small-molecule metabolites) in different areas of the horse’s hindgut. The study suggests that fecal samples may not fully represent the microbiome of certain hindgut regions like the caecum, which might have implications for scientific investigations of the horse’s digestive system.

Microbial Population Community Structure and Their Functional Metabolic Products

  • The study compares the microbiome and metabolome of fecal samples to those from the caecum and right dorsal colon of horses. The key method of comparison was the investigation of the microbial population community structure and their functional metabolic products.
  • 16S rRNA gene dendrograms were used to illustrate the distinct microbiome of the caecum, which was found to cluster separately from the other gut regions. The 16S rRNA gene-based quantitative PCR (q-PCR) also underlined these differences.
  • By using Fourier transform infrared (FTIR) spectroscopy, metabolites were identified and found to show a similar pattern of clustering, reinforcing that microbial composition and metabolites vary across different gut regions.

Protozoal and Archaeal Concentrations

  • Using q-PCR, the concentration of protozoal 18S rDNA and archaeal mcrA gene were quantified and found in higher numbers in the colon compared to other gut regions.

Diversity Assessments

  • Diversity in the horse’s gut was quantified using the Simpson and Shannon-Wiener indices. These calculations revealed higher diversity of bacteria in the right dorsal colon and feces than in the caecum.

Implications

  • Collectively, the results suggest that fecal samples likely represent the microbial population of the right dorsal colon to some extent but not that of the caecum.
  • This implies that careful consideration is necessary when planning microbial investigations of the hindgut of the horse, as the microbial population and metabolic products can significantly vary across different parts of the hindgut.

Cite This Article

APA
Dougal K, Harris PA, Edwards A, Pachebat JA, Blackmore TM, Worgan HJ, Newbold CJ. (2012). A comparison of the microbiome and the metabolome of different regions of the equine hindgut. FEMS Microbiol Ecol, 82(3), 642-652. https://doi.org/10.1111/j.1574-6941.2012.01441.x

Publication

FEMS microbiology ecology
ISSN: 1574-6941
NlmUniqueID: 8901229
Country: England
Language: English
Volume: 82
Issue: 3
Pages: 642-652

Researcher Affiliations

Dougal, Kirsty
  • Institute of Biological Environmental and Rural Sciences, Aberystwyth University, Penglais, Aberystwyth, Ceredigion, UK. kkd09@aber.ac.uk
Harris, Patricia A
    Edwards, Arwyn
      Pachebat, Justin A
        Blackmore, Tina M
          Worgan, Hilary J
            Newbold, C Jamie

              MeSH Terms

              • Animals
              • Archaea / genetics
              • Archaea / isolation & purification
              • Archaea / metabolism
              • Bacteria / genetics
              • Bacteria / isolation & purification
              • Cecum / metabolism
              • Cecum / microbiology
              • Colon / metabolism
              • Colon / microbiology
              • DNA, Ribosomal / genetics
              • Fatty Acids, Volatile / analysis
              • Fungi / genetics
              • Fungi / isolation & purification
              • Fungi / metabolism
              • Horses / microbiology
              • Metabolome
              • Metagenome
              • Polymerase Chain Reaction
              • RNA, Ribosomal, 18S / genetics

              Grant Funding

              • Biotechnology and Biological Sciences Research Council

              Citations

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