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Home / Equine Research Bank / Chem Phys Lipids / A fast method for the determination of the PC/LPC ratio in i...
Chemistry and physics of lipids2014; 183; 169-175; doi: 10.1016/j.chemphyslip.2014.07.001

A fast method for the determination of the PC/LPC ratio in intact serum by MALDI-TOF MS: an easy-to-follow lipid biomarker of inflammation.

Abstract: The PC/LPC ratio of blood serum is increasingly considered to represent an important clinical parameter that reflects various kinds of diseases. Here, a simple and fast method of lipid analyses of "intact" blood serum (i.e. without extraction) by MALDI-TOF mass spectrometry is described. The novel procedure allows the accurate determination of the PC/LPC ratio, utilizing only a tiny amount of blood. The serum is diluted with distilled water and directly applied onto the MALDI target and, after drying, covered by a thin layer of the matrix solution (either 9-aminoacridine or 2,5-dihydroxybenzoic acid). Positive ion mass spectra acquired by using this procedure give similar peak patterns as the spectra of the lipid extracts of horse blood serum. Blood serum from fourteen different horses was used to set up and validate the new method of lipid analysis. The PC/LPC ratios determined with the fast "intact" method were compared with those obtained with classical MALDI-TOF MS and (31)P NMR analyses of the corresponding lipid extracts. As comparable data were obtained, this is a clear indication that extraction is not an absolute necessity.
Copyright © 2014. Published by Elsevier Ireland Ltd.
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Publication Date: 2014-07-09 PubMed ID: 25016154DOI: 10.1016/j.chemphyslip.2014.07.001Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

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The article presents a novel, quick method for analyzing the PC/LPC ratio in blood serum using MALDI-TOF mass spectrometry without the need for extraction, which helps detecting various diseases using a very small blood sample.

Introduction

  • The researchers introduce the clinical significance of the Phosphatidylcholine to Lysophosphatidylcholine (PC/LPC) ratio in blood serum in reflecting various diseases.
  • They propose a faster, simpler method for lipid analysis using a Matrix-Assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) mass spectrometry.
  • This new method analyzes “intact” i.e. unprocessed, non-extracted blood serum and requires only a minuscule amount of blood.

Methodology

  • The blood serum is simply diluted with distilled water, directly placed on the MALDI target, and covered with a thin layer of the matrix solution using either 9-aminoacridine or 2,5-dihydroxybenzoic acid.
  • The mass spectra acquired through this method gave similar peak patterns as spectra obtained from the lipid extracts of horse blood serum, indicating the validity of this approach.
  • Blood serum from fourteen different horses was used to validate this new method of lipid analysis.

Results and Conclusion

  • The PC/LPC ratios determined with this new “intact” method were compared to those obtained with classical MALDI-TOF MS and (31)P NMR analyses of the corresponding lipid extracts.
  • The data obtained via both the methods were similar, demonstrating that the lengthy extraction process is not necessarily needed for accurate determination of the PC/LPC ratio.
  • This new method simplifies the blood serum lipid analysis process, reduces the amount of blood needed, and speeds up the analysis, thus having potential clinical implications in the early detection and management of inflammatory and other diseases.

Cite This Article

APA
(2014). A fast method for the determination of the PC/LPC ratio in intact serum by MALDI-TOF MS: an easy-to-follow lipid biomarker of inflammation. Chem Phys Lipids, 183, 169-175. https://doi.org/10.1016/j.chemphyslip.2014.07.001

Publication

Chemistry and physics of lipids
ISSN: 1873-2941
NlmUniqueID: 0067206
Country: Ireland
Language: English
Volume: 183
Pages: 169-175
PII: S0009-3084(14)00092-9

Researcher Affiliations

MeSH Terms

  • Animals
  • Biomarkers / blood
  • Horses
  • Inflammation / blood
  • Lysophosphatidylcholines / blood
  • Phosphatidylcholines / blood
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Spectrometry, Mass, Electrospray Ionization / methods
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods

Citations

This article has been cited 15 times.
  1. Grigorov I, Pejić S, Todorović A, Drakulić D, Veljković F, Vukajlović JM, Bobić K, Soldatović I, Đurašević S, Jasnić N, Stanković S, Glumac S, Mihailović-Vučinić V, Milenković B. Serum High-Mobility Group Box 1 and Heme Oxygenase-1 as Biomarkers in COVID-19 Patients at Hospital Admission. Int J Mol Sci 2023 Aug 24;24(17).
    doi: 10.3390/ijms241713164pubmed: 37685970google scholar: lookup
  2. Pina AF, Meneses MJ, Carli F, Ribeiro RT, Gardete-Correia L, Boavida JM, Raposo JF, Gastaldelli A, Macedo MP. Lipidomic profiling unveils sex differences in diabetes risk: Implications for precision medicine. Eur J Clin Invest 2026 Jan;56(1):e70137.
    doi: 10.1111/eci.70137pubmed: 41133433google scholar: lookup
  3. Palazzo C, Asci I, Russo S, Buccoliero C, Mangialardi V, Abbrescia P, Valente O, Ruggieri M, Paolicelli D, Lobasso S, Frigeri A. Circulating exosomes with unique lipid signature in relapsing remitting multiple sclerosis. Front Cell Neurosci 2025;19:1613618.
    doi: 10.3389/fncel.2025.1613618pubmed: 40656682google scholar: lookup
  4. Cao PP, Hu CL, Li MJ, An YH, Feng X, Ma XH, Wang DZ, Song ZH, Ji GS, Yang D, Ma Q, Yang WF, Dong JN, Zhang HR, Ma Y, Ma YF. 16S rRNA and metabolomics reveal the key microbes and key metabolites that regulate diarrhea in Holstein male calves. Front Microbiol 2024;15:1521719.
    doi: 10.3389/fmicb.2024.1521719pubmed: 39881985google scholar: lookup
  5. Bedoya-Guzmán FA, Pacheco-Herrero M, Salomon-Cruz ID, Barrera-Sandoval AM, Gutierrez Vargas JA, Villamil-Ortiz JG, Villegas Lanau CA, Arias-Londoño JD, Area-Gomez E, Cardona Gomez GP. BACE1 and SCD1 are associated with neurodegeneration. Front Aging Neurosci 2023;15:1194203.
    doi: 10.3389/fnagi.2023.1194203pubmed: 37744400google scholar: lookup
  6. Rees A, Edwards-I-Coll Z, Richards O, Raikes ME, Angelini R, Thornton CA. The dynamic inflammatory profile of pregnancy can be monitored using a novel lipid-based mass spectrometry technique. Mol Omics 2023 May 9;19(4):340-350.
    doi: 10.1039/d2mo00294apubmed: 36883215google scholar: lookup
  7. Bansal S, Bansal S, Fish BL, Li Y, Xu X, Fernandez JA, Griffin JH, Himburg HA, Boerma M, Medhora M, Cheema AK. Analysis of the urinary metabolic profiles in irradiated rats treated with Activated Protein C (APC), a potential mitigator of radiation toxicity. Int J Radiat Biol 2023;99(7):1109-1118.
    doi: 10.1080/09553002.2023.2182001pubmed: 36827630google scholar: lookup
  8. Shen L, Shen Y, You L, Zhang Y, Su Z, Peng G, Deng J, Zuo Z, Zhong Z, Ren Z, Yu S, Zong X, Zhu Y, Cao S. Pueraria lobata polysaccharides alleviate neonatal calf diarrhea by modulating gut microbiota and metabolites. Front Vet Sci 2022;9:1024392.
    doi: 10.3389/fvets.2022.1024392pubmed: 36686167google scholar: lookup
  9. Mocciaro G, D'Amore S, Jenkins B, Kay R, Murgia A, Herrera-Marcos LV, Neun S, Sowton AP, Hall Z, Palma-Duran SA, Palasciano G, Reimann F, Murray A, Suppressa P, Sabbà C, Moschetta A, Koulman A, Griffin JL, Vacca M. Lipidomic Approaches to Study HDL Metabolism in Patients with Central Obesity Diagnosed with Metabolic Syndrome. Int J Mol Sci 2022 Jun 17;23(12).
    doi: 10.3390/ijms23126786pubmed: 35743227google scholar: lookup
  10. Li YP, Wang CY, Shang HT, Hu RR, Fu H, Xiao XF. A high-throughput and untargeted lipidomics approach reveals new mechanistic insight and the effects of salvianolic acid B on the metabolic profiles in coronary heart disease rats using ultra-performance liquid chromatography with mass spectrometry. RSC Adv 2020 Apr 29;10(29):17101-17113.
    doi: 10.1039/d0ra00049cpubmed: 35521479google scholar: lookup
  11. Li X, Nakayama K, Goto T, Kimura H, Akamatsu S, Hayashi Y, Fujita K, Kobayashi T, Shimizu K, Nonomura N, Ogawa O, Inoue T. High level of phosphatidylcholines/lysophosphatidylcholine ratio in urine is associated with prostate cancer. Cancer Sci 2021 Oct;112(10):4292-4302.
    doi: 10.1111/cas.15093pubmed: 34328656google scholar: lookup
  12. Zhou R, He D, Xie J, Zhou Q, Zeng H, Li H, Huang L. The Synergistic Effects of Polysaccharides and Ginsenosides From American Ginseng (Panax quinquefolius L.) Ameliorating Cyclophosphamide-Induced Intestinal Immune Disorders and Gut Barrier Dysfunctions Based on Microbiome-Metabolomics Analysis. Front Immunol 2021;12:665901.
    doi: 10.3389/fimmu.2021.665901pubmed: 33968068google scholar: lookup
  13. Surowiec I, Ärlestig L, Rantapää-Dahlqvist S, Trygg J. Metabolite and Lipid Profiling of Biobank Plasma Samples Collected Prior to Onset of Rheumatoid Arthritis. PLoS One 2016;11(10):e0164196.
    doi: 10.1371/journal.pone.0164196pubmed: 27755546google scholar: lookup
  14. Wang G, Li Z, Li H, Li L, Li J, Yu C. Metabolic Profile Changes of CCl₄-Liver Fibrosis and Inhibitory Effects of Jiaqi Ganxian Granule. Molecules 2016 May 30;21(6).
    doi: 10.3390/molecules21060698pubmed: 27248993google scholar: lookup
  15. Angelini R, Lobasso S, Gorgoglione R, Bowron A, Steward CG, Corcelli A. Cardiolipin fingerprinting of leukocytes by MALDI-TOF/MS as a screening tool for Barth syndrome. J Lipid Res 2015 Sep;56(9):1787-94.
    doi: 10.1194/jlr.D059824pubmed: 26144817google scholar: lookup
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