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Home / Equine Research Bank / Int J Parasitol / A molecular systematic framework for equine strongyles based...
International journal for parasitology2000; 30(1); 95-103; doi: 10.1016/s0020-7519(99)00166-6

A molecular systematic framework for equine strongyles based on ribosomal DNA sequence data.

Abstract: In this study, molecular data sets were used to address the controversies relating to the systematics of strongyloid nematodes of equids utilising morphological data sets. DNA sequences of the first and second internal transcribed spacers (ITS-1 and ITS-2) of ribosomal DNA were determined for 30 species of equine strongyles and the systematic relationships reconstructed using phenetic and phylogenetic tree-building methods. The molecular data provided support for the hypothesis that the genera with large subglobular buccal capsules are ancestral to those with small cylindrical buccal capsules, but did not provide support for the current division of the subfamilies Strongylinae and Cyathostominae or for some taxonomic groupings (i.e. generic designations of species) within the Cyathostominea based on morphological data. Although not entirely concordant, the current molecular data provide a systematic framework for future studies of equine strongyles, which could be exploited in combination with new, phylogenetically informative morphological data sets.
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Publication Date: 2000-02-17 PubMed ID: 10675750DOI: 10.1016/s0020-7519(99)00166-6Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study is an exploration into the systematics of horse-strongyles, which are a type of nematode. The researchers utilized molecular data to investigate the hypothesis that specific genera of the strongyles are ancestral based on certain physical characteristics, and also evaluated support for current taxonomic categories.

Methodology

  • The researchers used DNA sequences of the first and second internal transcribed spacers (ITS-1 and ITS-2) of ribosomal DNA from 30 species of equine strongyles.
  • Using this molecular data set, they then reconstructed the systematic relationships between the different species.
  • Phenetic and phylogenetic tree-building methods were used to build these systematic relationships.

Findings

  • The molecular data supported the hypothesis that the genera of strongyles with large subglobular buccal capsules are ancestral to those with small cylindrical buccal capsules.
  • However, the molecular data did not support the current categorization of strongyles into the subfamilies Strongylinae and Cyathostominae. It also did not support some taxonomic groups within the Cyathostominea subfamily that were based on morphological data.

Implications

  • While there were some incongruent findings, this research provides a molecular systematic framework for future studies of equine strongyles.
  • This framework could be valuable when applied in combination with new, phylogenetically informative morphological datasets to reassess current taxonomic designations and to gain further insight into the evolution and phylogeny of equine strongyles.

Cite This Article

APA
Hung GC, Chilton NB, Beveridge I, Gasser RB. (2000). A molecular systematic framework for equine strongyles based on ribosomal DNA sequence data. Int J Parasitol, 30(1), 95-103. https://doi.org/10.1016/s0020-7519(99)00166-6

Publication

International journal for parasitology
ISSN: 0020-7519
NlmUniqueID: 0314024
Country: England
Language: English
Volume: 30
Issue: 1
Pages: 95-103

Researcher Affiliations

Hung, G C
  • Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Australia.
Chilton, N B
    Beveridge, I
      Gasser, R B

        MeSH Terms

        • Animals
        • DNA, Helminth / genetics
        • DNA, Ribosomal / genetics
        • Equidae / parasitology
        • Molecular Sequence Data
        • Phylogeny
        • Strongyloidea / classification
        • Strongyloidea / genetics

        Citations

        This article has been cited 19 times.
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