A monoclonal antibody identifying a T-cell marker in the horse.
Abstract: A cell surface molecule of equine T lymphocytes was identified and characterized using a mouse monoclonal antibody, HT23A. The molecule was detected on all T cells but not on other cells in peripheral blood, with the possible exception of a small subpopulation (about 5%) of B cells, as assessed by indirect immunofluorescence and flow cytometry. HT23A labelled T cell areas of horse lymph nodes and spleen when used in an indirect immunoperoxidase assay on frozen sections. Macrophages and neutrophils were not labelled by the antibody nor were frozen sections of horse liver, kidney, or brain. HT23A precipitated a molecule of approximately 69 kDa from 125Iodine labelled horse lymphocytes.
Publication Date: 1988-01-01 PubMed ID: 3069011DOI: 10.1111/j.1365-2052.1988.tb00826.xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Research Support
- U.S. Gov't
- Non-P.H.S.
- Research Support
- U.S. Gov't
- P.H.S.
Summary
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The research article presents the identification and characterization of a T-cell surface molecule in horses using a specific mouse monoclonal antibody, HT23A, which has potential implications in the field of immunology and veterinary science.
Identification of Equine T-Lymphocyte Marker
- The research focuses on a particular T-cell surface molecule in horses. T-cells are a type of white blood cell crucial in the immune response of animals, including humans and horses.
- To investigate this, the scientists used a mouse monoclonal antibody called HT23A. Monoclonal antibodies are lab-made proteins that can bind to specific substances; in this case, the HT23A antibody was designed to bind to an unidentified molecule on the surface of equine (horse) T-cells.
Characterization of the Surface Molecule
- This surface molecule was found on all T-cells and potentially on a small subset (around 5%) of B-cells, another type of immune cell. However, it was not present on other cell types in the horse’s peripheral blood.
- These findings were confirmed through indirect immunofluorescence and flow cytometry, laboratory techniques that allow for the visualization and sorting of cells, respectively.
- Moreover, the HT23A also labeled T-cell areas in horse lymph nodes and spleen when used in an indirect immunoperoxidase assay on frozen sections, reinforcing its selectivity for T-cells.
Specificity of HT23A
- Macrophages and neutrophils, other types of white blood cells, were not labeled with the HT23A antibody. The same was true for frozen sections of horse liver, kidney, or brain. This signifies that the surface molecule distinguished by HT23A is specific mainly for T-cells.
- In addition, HT23A precipitated a molecule of approximately 69 kDa (Kilodalton – a unit of molecular weight) from iodine-125 labeled horse lymphocytes, which could refer to the molecular weight of the surface molecule.
Significance and Implications
- The significance of this research lies in understanding the immunological features of horses and potentially of other species. The detected surface molecule could play a vital role in immune response, disease progression, or vaccine development.
- The specificity of HT23A for T-cell surface molecule potentially makes it a valuable tool in both equine immunology research, veterinary medicine, and potentially in comparative immunology.
Cite This Article
APA
Crump AL, Davis W, Antczak DF.
(1988).
A monoclonal antibody identifying a T-cell marker in the horse.
Anim Genet, 19(4), 349-357.
https://doi.org/10.1111/j.1365-2052.1988.tb00826.x Publication
Researcher Affiliations
- James A. Baker Institute for Animal Health, New York State College of Veterinary Medicine, Cornell University, Ithaca 14853.
MeSH Terms
- Animals
- Antibodies, Monoclonal / immunology
- Antigens, Differentiation, B-Lymphocyte / immunology
- Antigens, Differentiation, T-Lymphocyte / immunology
- Biotin
- Horses / immunology
- Immunoenzyme Techniques / veterinary
- Molecular Weight
Grant Funding
- HD-15799 / NICHD NIH HHS
Citations
This article has been cited 4 times.- Carossino M, Loynachan AT, Canisso IF, Cook RF, Campos JR, Nam B, Go YY, Squires EL, Troedsson MHT, Swerczek T, Del Piero F, Bailey E, Timoney PJ, Balasuriya UBR. Equine Arteritis Virus Has Specific Tropism for Stromal Cells and CD8(+) T and CD21(+) B Lymphocytes but Not for Glandular Epithelium at the Primary Site of Persistent Infection in the Stallion Reproductive Tract.. J Virol 2017 Jul 1;91(13).
- Mérant C, Messouak A, Cadoré JL, Monier JC. PNA-binding glycans are expressed at high levels on horse mature and immature T lymphocytes and a subpopulation of B lymphocytes.. Glycoconj J 2005 Feb;22(1-2):27-34.
- Blanchard-Channell M, Moore PF, Stott JL. Characterization of monoclonal antibodies specific for equine homologues of CD3 and CD5.. Immunology 1994 Aug;82(4):548-54.
- Lunn DP, Holmes MA, Duffus WP. Three monoclonal antibodies identifying antigens on all equine T lymphocytes, and two mutually exclusive T-lymphocyte subsets.. Immunology 1991 Oct;74(2):251-7.
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