[A multiplex PCR method based on nuclear and cytoplasmic inheritance to identify the horse and donkey-derived components of Asini Colla Corii and the hide].

This research focuses on effectively identifying the original components of Asini Colla Corii and the hides used as its raw materials. It has established a multiplex PCR method to distinguish horse, donkey, mule, and hinny hides, thus ensuring the authenticity of Asini Colla Corii.

Identification of Asini Colla Corii Components

• The objective of the study is to enhance the authenticity of Asini Colla Corii, a product made from equine hides, by establishing effective identification methods.
• Asini Colla Corii production businesses and market supervision departments need effective methods for identifying original components such as horse, donkey, mule, and hinny hides.

Use of Multiplex PCR Method

• To achieve this, the study uses a multiplex PCR method, a process that has been developed to identify multiple targets in a single PCR experiment.
• This method focuses on distinguishing between specific DNA sequences of different equine species’ nuclear and mitochondrial genomes.
• The researchers designed specific primers for horse and donkey hides that serve as key identifiers.

Results of the Study

• Through the multiplex PCR method, the study successfully identifies and differentiates among horse, donkey, mule, and hinny hides.
• This method determines the species of hide used in Asini Colla Corii with a high level of specificity, reliable enough to prevent cross-amplification – a problem that arises when the PCR process unintentionally multiplies non-target DNA sequences.
• The limit of detection of this method stands at 0.2 ng DNA, indicating its high sensitivity.

Significance of the Study

• This research provides technical guidance and infrastructure for Asini Colla Corii production enterprises and market supervision departments, helping them authenticate the original components of their product.
• By ensuring the validity of raw materials and the finished product, this work aims to maintain quality control and build consumers’ trust in Asini Colla Corii.