A new method for the isolation of aminoacylase from mammalian kidneys.

The research explores a novel approach to isolate the enzyme aminoacylase from mammalian kidneys. The authors extracted the enzyme from different mammalian species and further purified it, demonstrating that it can be used for practical purposes.

Methodology

The researchers developed a method to isolate the enzyme aminoacylase. This involves:

• Extracting the enzyme from the kidneys of different mammalian species, namely horses, cattle, rabbits, and pigs.
• The extraction process involves treating the kidney with water and thereafter, subjecting the extract to heat treatment at 70 degrees Celcius for 10 minutes.

Protein Fractionation Process

After heating, the denatured or non-functional proteins are removed. This is followed by:

• Fractionating the remaining proteins in the solution by using ammonium sulfate, a common method for protein purification.
• The end result of this process is the isolated aminoacylase enzyme.

Practical Application and Further Purification

The isolated enzyme has potential practical applications and can be used directly for these. One such application mentioned in the study is for the preparation of immobilized aminoacylase. However, for further purification, the study describes a combination of ion exchange chromatography and gel filtration, particularly for the purification of aminoacylase from porcine (pig) kidneys.

Significance

This study provides a new methodology for the isolation of the aminoacylase enzyme from mammalian kidneys. The innovative part of the research lies in the method to isolate and purify this enzyme, which can potentially expedite research and practical applications that rely on this particular enzyme. The research also opens avenues for further exploration in enzyme isolation techniques from mammalian organs.