A polarized light microscopy method for accurate and reliable grading of collagen organization in cartilage repair.
Abstract: Collagen organization, a feature that is critical for cartilage load bearing and durability, is not adequately assessed in cartilage repair tissue by present histological scoring systems. Our objectives were to develop a new polarized light microscopy (PLM) score for collagen organization and to test its reliability. Methods: This PLM score uses an ordinal scale of 0-5 to rate the extent that collagen network organization resembles that of young adult hyaline articular cartilage (score of 5) vs a totally disorganized tissue (score of 0). Inter-reader reliability was assessed using Intraclass Correlation Coefficients (ICC) for Agreement, calculated from scores of three trained readers who independently evaluated blinded sections obtained from normal (n=4), degraded (n=2) and repair (n=22) human cartilage biopsies. Results: The PLM score succeeded in distinguishing normal, degraded and repair cartilages, where the latter displayed greater complexity in collagen structure. Excellent inter-reader reproducibility was found with ICCs for Agreement of 0.90 [ICC(2,1)] (lower boundary of the 95% confidence interval is 0.83) and 0.96 [ICC(2,3)] (lower boundary of the 95% confidence interval is 0.94), indicating the reliability of a single reader's scores and the mean of all three readers' scores, respectively. Conclusions: This PLM method offers a novel means for systematically evaluating collagen organization in repair cartilage. We propose that it be used to supplement current gold standard histological scoring systems for a more complete assessment of repair tissue quality.
Copyright © 2010 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.
Publication Date: 2010-10-16 PubMed ID: 20955805DOI: 10.1016/j.joca.2010.10.010Google Scholar: Lookup
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Summary
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The research article describes the development and evaluation of a new polarized light microscopy (PLM) scoring system for assessing collagen organization in cartilage repair tissue. The score ranges from 0 to 5, with 5 resembling young healthy cartilage and 0 representing thoroughly disorganized tissue. The authors demonstrated that the PLM score is both accurate and reliable, making it a potentially valuable addition to current techniques for evaluating tissue repair quality.
Methodology
- The researchers developed a PLM score that uses an ordinal scale from 0 to 5. A score of 5 indicates collagen network organization similar to that of young adult hyaline articular cartilage, while a score of 0 signifies completely disorganized tissue.
- They then assessed the inter-reader reliability of the PLM score, utilizing Intraclass Correlation Coefficients (ICC) for Agreement. This occurred by having three trained readers independently evaluate blinded sections derived from normal, degraded, and repair human cartilage biopsies.
Results
- The PLM score successfully distinguished between normal, degraded, and repair cartilages, showing greater complexity in the collagen structure of the repair cartilages.
- Excellent inter-reader reproducibility was found. The ICCs for Agreement were 0.90 (with the lower boundary of the 95% confidence interval being 0.83) and 0.96 (lower boundary of the 95% confidence interval being 0.94). These indicate the reliability of both a single reader’s scores and the mean of all three readers’ scores, respectively.
Conclusions
- The authors concluded that the PLM method offers a new way of systematically evaluating collagen organization in repair cartilage, which is an important factor for cartilage load bearing and durability.
- They suggested that this method could supplement the current gold standard histological scoring systems, thereby providing a more thorough assessment of repair tissue quality.
Cite This Article
APA
Changoor A, Tran-Khanh N, Méthot S, Garon M, Hurtig MB, Shive MS, Buschmann MD.
(2010).
A polarized light microscopy method for accurate and reliable grading of collagen organization in cartilage repair.
Osteoarthritis Cartilage, 19(1), 126-135.
https://doi.org/10.1016/j.joca.2010.10.010 Publication
Researcher Affiliations
- Department of Chemical Engineering and Institute of Biomedical Engineering, École Polytechnique de Montréal, P.O. Box 6079, Station Centre-Ville, Montreal, Québec H3C 3A7, Canada.
MeSH Terms
- Adolescent
- Adult
- Age Distribution
- Animals
- Cartilage, Articular / injuries
- Cartilage, Articular / ultrastructure
- Collagen / ultrastructure
- Female
- Horses
- Humans
- Knee Joint / pathology
- Male
- Microscopy, Polarization / instrumentation
- Microscopy, Polarization / methods
- Middle Aged
- Observer Variation
- Reproducibility of Results
- Young Adult
Grant Funding
- Canadian Institutes of Health Research
Citations
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