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A scanning electron microscopic study of platelets of certain animal species.

Abstract: Comparative morphology of platelets of the dog, cat, cow, horse, sheep, and goat was studied with the scanning electron microscope and was found generally similar. Most of the platelets were slightly biconvex of flat with relatively smooth surface and even contour. Some of these platelets had few short marginal filaments, oriny protuberances on the surface. A small number of platelets exhibited surface irregularity and few long pseudopods which were thought to result from platelet activation during the processing of blood. The number os such irregular platelets increased in stored blood and in blood fixed at 4 degrees C.
Publication Date: 1975-06-30 PubMed ID: 1154309
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  • Comparative Study
  • Journal Article
  • Research Support
  • U.S. Gov't
  • Non-P.H.S.

Summary

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The research paper discusses a study investigating the morphology of animal platelets, particularly concerning dogs, cats, cows, horses, sheep, and goats, using a scanning electron microscope.

Objective of the Research

  • The main objective of this research was to compare the morphology, or structure, of platelets from different animal species including dogs, cats, cows, horses, sheep, and goats. This was done using a Scanning Electron Microscope, a device that allows scientists to observe minute details of biological samples.

Method and Observations

  • Most observed platelets were found to be slightly biconvex or flat having a relatively smooth surface and even contour, which means they have two outwardly curved surfaces with a rounded, orderly shape and smooth texture.
  • Some of these platelets exhibited short marginal filaments or tiny protrusions on their surface, understood as minuscule hair-like structures or small bumps that extend outward.
  • A few platelets showed irregularities on the surface and had long pseudopods, which are temporary, arm-like projections that are a sign of platelet activation, thought to be resulting from the process of handling the blood sample for study.

Factors Affecting Platelet Structure

  • The study observed an increase in the number of irregular platelets when stored blood was used or when blood was fixed at a low temperature of 4 degrees Celsius.
  • This suggests that the conditions in which blood samples are stored and processed can influence the morphology of the platelets they contain, particularly causing the formation of pseudopods, warning a note of caution for researchers to control these variables in their studies.

Conclusion

  • The overall observation indicated a broad similarity in the morphology of platelets across the various animal species studied, although certain specific distinguishing characteristics were also noted.
  • This kind of comparative study is important, as it helps in the understanding of similarities and differences at the cellular level across species, which can have implications for comparative physiology, disease studies, and animal medicine.

Cite This Article

APA
Jain NC. (1975). A scanning electron microscopic study of platelets of certain animal species. Thromb Diath Haemorrh, 33(3), 501-507.

Publication

ISSN: 0340-5338
NlmUniqueID: 7608420
Country: Germany
Language: English
Volume: 33
Issue: 3
Pages: 501-507

Researcher Affiliations

Jain, N C

    MeSH Terms

    • Animals
    • Blood Platelets / ultrastructure
    • Blood Preservation
    • Cats
    • Cattle
    • Dogs
    • Goats
    • Horses
    • Microscopy, Electron, Scanning
    • Pseudopodia / ultrastructure
    • Sheep
    • Species Specificity
    • Temperature

    Citations

    This article has been cited 3 times.
    1. Nievergelt AP, Brillard C, Eskandarian HA, McKinney JD, Fantner GE. Photothermal Off-Resonance Tapping for Rapid and Gentle Atomic Force Imaging of Live Cells. Int J Mol Sci 2018 Sep 30;19(10).
      doi: 10.3390/ijms19102984pubmed: 30274330google scholar: lookup
    2. Calderone RA, Rotondo MF, Sande MA. Candida albicans endocarditis: ultrastructural studies of vegetation formation. Infect Immun 1978 Apr;20(1):279-89.
      doi: 10.1128/iai.20.1.279-289.1978pubmed: 352932google scholar: lookup
    3. Rieger H, Wurzinger L, Schmid-Schöbein H. [Influence of temperature on shear induced platelet aggregation in vitro (author's transl)]. Klin Wochenschr 1977 Feb 1;55(3):121-30.
      doi: 10.1007/BF01490239pubmed: 138019google scholar: lookup