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Home / Equine Research Bank / Anim Reprod / Action of swim-up and caffeine on equine frozen sperm.
Animal reproduction2022; 19(4); e20220056; doi: 10.1590/1984-3143-AR2022-0056

Action of swim-up and caffeine on equine frozen sperm.

Abstract: Cryopreservation of equine semen is crucial to semen commercialization. However, it reduces sperm motility and longevity. Thus, sperm selection methods and addition of motility-activating substances to sperm, such as caffeine, may improve sperm quality of equine frozen semen. The objective of the current work was to evaluate the effects of caffeine on recovery and quality parameters of frozen-thawed sperm subjected to swim-up selection to be used in intracytoplasmic sperm injection (ICSI) in assisted reproductive techniques. Stallion semen were frozen and after thawing different caffeine concentrations were added to the samples performing four treatments control (no caffeine), 3, 5, and 7.5 mM caffeine. Sperm kinematic and motility were assessed by computer-assisted sperm analysis (CASA). Then, the four treated samples were submitted to the swim-up sperm selection, and the number of recovered sperm and morphology were evaluated at four times 20, 40, 60, and 80 min. The swim-up increased the recovery proportion of normal morphology sperm without (80.1±1%) or with caffeine addition (3mM: 81.2±1%, 5mM: 79.9±1% and 7.5 mM 78.9±1%) compared to the thawed semen (70±2%). However, the addition of 5 mM caffeine induced an increase in sperm motility (38.9±2.8 vs. 32.6±3.4%, P<0.05), and sperm recovery after swim-up (7.9x106 vs. 3.4x106 sperm/ml, P<0.05) compared to the control. The addition of 5 mM caffeine to frozen-thawed equine semen before swim-up selection improved sperm motility and increased the sperm recovery rate while not decreasing the percentage of morphologically normal sperm. Thus, caffeine addition to frozen-thawed equine semen before swim-up selection has potential clinical application in improving sperm quality for use in ICSI.
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Publication Date: 2022-11-21 PubMed ID: 36504918PubMed Central: PMC9706678DOI: 10.1590/1984-3143-AR2022-0056Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates the impact of adding different concentrations of caffeine to frozen-thawed stallion sperm and the resulting effects on sperm motility, morphology and recovery rate, particularly for use in equine intracytoplasmic sperm injection (ICSI).

Objective and Methodology

  • The study aims to discover if adding caffeine to frozen-thawed sperm from stallions could improve the sperm’s quality. The method of ‘swim-up selection’ is used, which is a type of sperm selection process widely used in assisted reproductive techniques.
  • Four different treatments were tested for this study: a control group (no caffeine), and three groups with varying concentrations of caffeine – 3mM, 5mM, and 7.5mM.
  • The study used Computer-Assisted Sperm Analysis (CASA) to assess sperm kinematic and motility following thawing and treatment with different caffeine concentrations.
  • Each sample, post-treatment, was submitted to the swim-up sperm selection process. The number of recovered sperm and their morphology were evaluated at four distinct time intervals: 20, 40, 60, and 80 minutes.

Findings

  • Results revealed that swim-up selection increased the recovery proportion of normal morphology sperm. This increase was seen both in the control group (80.1%±1%) and the caffeine groups (3mM: 81.2%±1%, 5mM: 79.9%±1%, 7.5mM: 78.9%±1%) when compared to the thawed semen (70±2%).
  • The study found that the addition of 5mM caffeine significantly increased sperm motility (38.9%±2.8 vs. 32.6%±3.4%, P<0.05), and recovery rate post swim-up (7.9×10 vs. 3.4×10 sperm/ml, P<0.05) when compared to the control group.
  • Also important is that the enhancement in sperm quality seen with the 5mM caffeine concentration did not result in a decrease in the percentage of morphologically normal sperm.

Conclusion

  • The research found that by adding 5mM caffeine to frozen-thawed equine semen before performing swim-up selection, there was an improvement in sperm motility and an increase in the sperm recovery rate.
  • Given these results, it is evident that this process may have potential clinical applications for improving the quality of sperm used in ICSI, a common procedure in assisted reproduction.

Cite This Article

APA
Alves NC, Diniz SA, Viegas RN, Arigoni AL, Freitas MM, Lana ÂQ, Lagares MA. (2022). Action of swim-up and caffeine on equine frozen sperm. Anim Reprod, 19(4), e20220056. https://doi.org/10.1590/1984-3143-AR2022-0056

Publication

Animal reproduction
ISSN: 1984-3143
NlmUniqueID: 101272804
Country: Brazil
Language: English
Volume: 19
Issue: 4
Pages: e20220056

Researcher Affiliations

Alves, Natália de Castro
  • Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
Diniz, Soraia de Araújo
  • Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
Viegas, Rodrigo Novaes
  • Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
Arigoni, Ana Luiza
  • Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
Freitas, Marina Morra
  • Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
Lana, Ângela Quintão
  • Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
Lagares, Monique de Albuquerque
  • Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.

Conflict of Interest Statement

Conflicts of interest: The authors have no conflict of interest to declare.

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Citations

This article has been cited 1 times.
  1. Ďuračka M, Benko F, Chňapek M, Tvrdá E. Strategies for Bacterial Eradication from Human and Animal Semen Samples: Current Options and Future Alternatives. Sensors (Basel) 2023 Aug 6;23(15).
    doi: 10.3390/s23156978pubmed: 37571761google scholar: lookup
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