Actions of a nitric oxide donor on prostaglandin production and angiogenic activity in the equine endometrium.
Abstract: Nitric oxide (NO) plays an important role in prostaglandin secretion and angiogenesis in the reproductive system. In the present study, the roles of the NO donor spermine NONOate and tumour necrosis factor-alpha (TNF; as a positive control) in prostaglandin production and angiogenic activity of equine endometria during the oestrous cycle were evaluated. In addition, the correlation between NO production and the expression of key prostaglandin synthase proteins was determined. The protein expression of prostaglandin F synthase (PGFS) increased in early and mid-luteal stages, whereas that of prostaglandin E synthase (PGES) was increased in the early luteal stage. The in vitro release of NO was highest after ovulation. There was a high correlation between NO production and PGES expression, as well as NO release and PGFS expression. There were no differences detected in prostaglandin H synthase 2 (PTGS-2) throughout the oestrous cycle and there was no correlation between PTGS-2 expression and NO. In TNF- or spermine-treated endometria, the expression of prostaglandin (PG) E(2) increased in the early and mid-luteal phases, whereas that of PGF(2alpha) increased in the follicular and late luteal phases. Bovine aortic endothelial cell (BAEC) proliferation was stimulated in TNF-treated follicular-phase endometria. However, in spermine-treated endometria, NO delivered from its donor had no effect, or even an inhibitory effect, on BAEC proliferation. In conclusion, despite no change in PTGS-2 expression throughout the oestrous cycle in equine endometrial tissue, there were changes observed in the expression of PGES and PGFS, as well as in the production of PGE(2) and PGF(2alpha). In the mare, NO is involved in the secretory function of the endometrium, modulating PGE(2) and PGF(2alpha) production. Even though TNF caused an increase in the production of angiogenic factors and prostaglandins, its complex action in mare uterus should be elucidated.
Publication Date: 2008-08-02 PubMed ID: 18671915DOI: 10.1071/rd08015Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research investigates the influence of nitric oxide, which is crucial for the secretion of prostaglandin and vascular formation, on equine endometrium during the oestrous cycle. Observations included a correlation between nitric oxide production and the expression of certain prostaglandin synthase proteins, but no observed changes in prostaglandin H synthase 2 throughout the oestrous cycle.
Nitric Oxide Donor and Tumour Necrosis Factor-Alpha Role
- The roles of the nitric oxide donor spermine NONOate and tumour necrosis factor-alpha were examined concerning the production of prostaglandin and angiogenic activity.
- Both of these compounds were observed in the equine endometria during the oestrous cycle.
Prostaglandin Protein Expression
- Prostaglandin F synthase protein expression was found to increase during certain stages of the luteal phase.
- The expression of prostaglandin E synthase also showed a significant increase in the early luteal stage.
Nitric Oxide Production and Release
- In vitro, the highest release of nitric oxide was observed after ovulation.
- Nitric oxide production and release showed a strong correlation with the expression of prostaglandin F and E synthases.
Effects on Prostaglandin H Synthase
- No noticeable differences were observed in prostaglandin H synthase 2 during the oestrous cycle.
- This synthase showed no correlation with nitric oxide.
Expression of Prostaglandin in Treated Endometria
- With tumour necrosis factor-alpha or spermine treatment, there was an increase in prostaglandin E(2) during the early and mid-luteal stages.
- The levels of PGF(2alpha) were also observed to have increased during the follicular and late luteal phases.
Influence on Bovine Aortic Endothelial Cell Proliferation
- Tumour necrosis factor-alpha treated endometria from the follicular phase exhibited a stimulation in BAEC proliferation.
- However, spermine-treated endometria showed no significant changes or even a decrease in BAEC proliferation, suggesting that nitric oxide received from donors may not affect or may inhibit the proliferation.
Conclusion & Future Directions
- The study concluded that changes in the expression of PGES and PGFS and the production of PGE(2) and PGF(2alpha) occur in endometrial tissue, even though PTGS-2 expression remains constant throughout the oestrous cycle.
- Nitric oxide was found to play a part in the secretory function of the endometrium by modulating PGE(2) and PGF(2alpha) production.
- Despite causing an increase in the production of angiogenic factors and prostaglandins, the complex action of TNF in horse uterus should be further investigated for a better understanding.
Cite This Article
APA
Roberto da Costa RP, Costa AS, Korzekwa AJ, Platek R, Siemieniuch M, Galvão A, Redmer DA, Silva JR, Skarzynski DJ, Ferreira-Dias G.
(2008).
Actions of a nitric oxide donor on prostaglandin production and angiogenic activity in the equine endometrium.
Reprod Fertil Dev, 20(6), 674-683.
https://doi.org/10.1071/rd08015 Publication
Researcher Affiliations
- Department of Animal Sciences, Escola Superior Agrria de Coimbra, Bencanta, Coimbra, Portugal.
MeSH Terms
- Animals
- Cattle
- Cell Proliferation / drug effects
- Cells, Cultured
- Endometrium / drug effects
- Endometrium / metabolism
- Endothelial Cells / drug effects
- Endothelial Cells / metabolism
- Endothelial Cells / physiology
- Estrous Cycle / drug effects
- Female
- Horses / metabolism
- Neovascularization, Physiologic / drug effects
- Neovascularization, Physiologic / physiology
- Nitric Oxide / pharmacology
- Nitric Oxide Donors / pharmacology
- Prostaglandin-Endoperoxide Synthases / metabolism
- Prostaglandins / biosynthesis
- Prostaglandins / physiology
- Tumor Necrosis Factor-alpha / pharmacology
Citations
This article has been cited 4 times.- Rivera Del Alamo MM, Reilas T, Lukasik K, Galvão AM, Yeste M, Katila T. Inflammatory Markers in Uterine Lavage Fluids of Pregnant, Non-Pregnant, and Intrauterine Device Implanted Mares on Days 10 and 15 Post Ovulation. Animals (Basel) 2021 Dec 8;11(12).
- Pinto-Bravo P, Rebordão MR, Amaral A, Fernandes C, Galvão A, Silva E, Pessa-Santos P, Alexandre-Pires G, Roberto da Costa RP, Skarzynski DJ, Ferreira-Dias G. Microvascularization and Expression of Fibroblast Growth Factor and Vascular Endothelial Growth Factor and Their Receptors in the Mare Oviduct. Animals (Basel) 2021 Apr 12;11(4).
- Zerani M, Polisca A, Boiti C, Maranesi M. Current Knowledge on the Multifactorial Regulation of Corpora Lutea Lifespan: The Rabbit Model. Animals (Basel) 2021 Jan 25;11(2).
- Lupicka M, Zadroga A, Szczepańska A, Korzekwa AJ. Effect of ovarian steroids on vascular endothelial growth factor a expression in bovine uterine endothelial cells during adenomyosis. BMC Vet Res 2019 Dec 30;15(1):473.
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