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Home / Equine Research Bank / J Bone Joint Surg Am / Addition of Mesenchymal Stem Cells to Autologous Platelet-En...
The Journal of bone and joint surgery. American volume2016; 98(1); 23-34; doi: 10.2106/JBJS.O.00407

Addition of Mesenchymal Stem Cells to Autologous Platelet-Enhanced Fibrin Scaffolds in Chondral Defects: Does It Enhance Repair?

Abstract: The chondrogenic potential of culture-expanded bone-marrow-derived mesenchymal stem cells (BMDMSCs) is well described. Numerous studies have also shown enhanced repair when BMDMSCs, scaffolds, and growth factors are placed into chondral defects. Platelets provide a rich milieu of growth factors and, along with fibrin, are readily available for clinical use. The objective of this study was to determine if the addition of BMDMSCs to an autologous platelet-enriched fibrin (APEF) scaffold enhances chondral repair compared with APEF alone. Methods: A 15-mm-diameter full-thickness chondral defect was created on the lateral trochlear ridge of both stifle joints of twelve adult horses. In each animal, one defect was randomly assigned to receive APEF+BMDMSCs and the contralateral defect received APEF alone. Repair tissues were evaluated one year later with arthroscopy, histological examination, magnetic resonance imaging (MRI), micro-computed tomography (micro-CT), and biomechanical testing. Results: The arthroscopic findings, MRI T2 map, histological scores, structural stiffness, and material stiffness were similar (p > 0.05) between the APEF and APEF+BMDMSC-treated repairs at one year. Ectopic bone was observed within the repair tissue in four of twelve APEF+BMDMSC-treated defects. Defects repaired with APEF alone had less trabecular bone edema (as seen on MRI) compared with defects repaired with APEF+BMDMSCs. Micro-CT analysis showed thinner repair tissue in defects repaired with APEF+BMDMSCs than in those treated with APEF alone (p < 0.05). Conclusions: APEF alone resulted in thicker repair tissue than was seen with APEF+BMDMSCs. The addition of BMDMSCs to APEF did not enhance cartilage repair and stimulated bone formation in some cartilage defects. Conclusions: APEF supported repair of critical-size full-thickness chondral defects in horses, which was not improved by the addition of BMDMSCs. This work supports further investigation to determine whether APEF enhances cartilage repair in humans.
Copyright © 2016 by The Journal of Bone and Joint Surgery, Incorporated.
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Publication Date: 2016-01-08 PubMed ID: 26738900PubMed Central: PMC4697360DOI: 10.2106/JBJS.O.00407Google Scholar: Lookup
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses a study that explored whether the addition of bone marrow-derived mesenchymal stem cells (BMDMSCs) to an autologous platelet-enriched fibrin (APEF) scaffold would enhance the repair of chondral defects (damage to cartilage) compared to using APEF alone. The study concluded that the addition of BMDMSCs did not markedly improve the results and, in some cases, even stimulated unwelcome bone formation in the cartilage defects.

Research Methodology

  • The study involved creating full-thickness chondral defects of 15mm diameter on the stifle joints (equivalent to the human knee) of twelve adult horses. These defects were then treated with either APEF alone, or a combination of APEF and BMDMSCs.
  • The treated areas were evaluated after a year using arthroscopy (examining joints using a type of endoscope), histological examination (looking at the microscopic structure of tissues), MRI scans, micro-computed tomography (a type of high-resolution 3D X-ray imaging), and biomechanical tests. Ectopic bone (bone growth in an abnormal location) was noted in four of the defects treated with the APEF + BMDMSCs combination.

Research Findings

  • Comparison of the two treatment methods did not indicate any significant differences in arthroscopic findings, MRI T2 maps, histological scores, material stiffness, and structural stiffness after a one-year period.
  • However, defects treated with the combined APEF and BMDMSCs treatment had thinner repair tissue and more trabecular bone edema (swelling caused by excess water) than those treated with APEF alone.

Conclusion

  • The researchers found that the APEF scaffold was effective in the repair of critical-size full-thickness chondral defects in horses, but the addition of BMDMSCs did not enhance the cartilage repair.
  • On the contrary, the addition of stem cells resulted in some unwanted bone formation and thinner repair tissue, recommending further investigation to determine whether APEF alone should be used to enhance cartilage repair in humans.

Cite This Article

APA
Goodrich LR, Chen AC, Werpy NM, Williams AA, Kisiday JD, Su AW, Cory E, Morley PS, McIlwraith CW, Sah RL, Chu CR. (2016). Addition of Mesenchymal Stem Cells to Autologous Platelet-Enhanced Fibrin Scaffolds in Chondral Defects: Does It Enhance Repair? J Bone Joint Surg Am, 98(1), 23-34. https://doi.org/10.2106/JBJS.O.00407

Publication

The Journal of bone and joint surgery. American volume
ISSN: 1535-1386
NlmUniqueID: 0014030
Country: United States
Language: English
Volume: 98
Issue: 1
Pages: 23-34

Researcher Affiliations

Goodrich, Laurie R
  • Gail Holmes Equine Orthopedic Research Center, Colorado State University, 300 West Drake Road, Fort Collins, CO 80523.
Chen, Albert C
  • Department of Bioengineering, Mail Code 0412, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0412.
Werpy, Natasha M
  • Large Animal Clinical Sciences, 2015 S.W. 16th Avenue, Gainesville, FL 32608.
Williams, Ashley A
  • Department of Orthopedic Surgery, Stanford University School of Medicine, 450 Broadway Street, Redwood City, CA 94063.
Kisiday, John D
  • Gail Holmes Equine Orthopedic Research Center, Colorado State University, 300 West Drake Road, Fort Collins, CO 80523.
Su, Alvin W
  • Department of Bioengineering, Mail Code 0412, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0412.
Cory, Esther
  • Department of Bioengineering, Mail Code 0412, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0412.
Morley, Paul S
  • Gail Holmes Equine Orthopedic Research Center, Colorado State University, 300 West Drake Road, Fort Collins, CO 80523.
McIlwraith, C Wayne
  • Gail Holmes Equine Orthopedic Research Center, Colorado State University, 300 West Drake Road, Fort Collins, CO 80523.
Sah, Robert L
  • Department of Bioengineering, Mail Code 0412, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0412.
Chu, Constance R
  • Department of Orthopedic Surgery, Stanford University School of Medicine, 450 Broadway Street, Redwood City, CA 94063. chucr@stanford.edu.

MeSH Terms

  • Animals
  • Arthroscopy / methods
  • Biopsy, Needle
  • Blood Platelets
  • Cartilage Diseases / pathology
  • Cartilage Diseases / surgery
  • Cartilage, Articular / pathology
  • Cartilage, Articular / surgery
  • Disease Models, Animal
  • Fibrin / administration & dosage
  • Fibrin / pharmacology
  • Follow-Up Studies
  • Horses
  • Humans
  • Immunohistochemistry
  • Magnetic Resonance Imaging / methods
  • Mesenchymal Stem Cell Transplantation / methods
  • Random Allocation
  • Tissue Engineering / methods
  • Tissue Scaffolds
  • Transplantation, Autologous
  • Treatment Outcome

Grant Funding

  • R01 AR052784 / NIAMS NIH HHS
  • R01 AR051963 / NIAMS NIH HHS
  • R01 AR044058 / NIAMS NIH HHS
  • RC2 AR058929 / NIAMS NIH HHS
  • 1K08AR054903-01A2 / NIAMS NIH HHS
  • P01 AG007996 / NIA NIH HHS

References

This article includes 50 references
  1. Wakitani S, Goto T, Pineda SJ, Young RG, Mansour JM, Caplan AI, Goldberg VM. Mesenchymal cell-based repair of large, full-thickness defects of articular cartilage.. J Bone Joint Surg Am 1994 Apr;76(4):579-92.
    pubmed: 8150826doi: 10.2106/00004623-199404000-00013google scholar: lookup
  2. McIlwraith CW, Frisbie DD, Rodkey WG, Kisiday JD, Werpy NM, Kawcak CE, Steadman JR. Evaluation of intra-articular mesenchymal stem cells to augment healing of microfractured chondral defects.. Arthroscopy 2011 Nov;27(11):1552-61.
    pubmed: 21862278doi: 10.1016/j.arthro.2011.06.002google scholar: lookup
  3. Centeno CJ, Busse D, Kisiday J, Keohan C, Freeman M, Karli D. Increased knee cartilage volume in degenerative joint disease using percutaneously implanted, autologous mesenchymal stem cells.. Pain Physician 2008 May-Jun;11(3):343-53.
    pubmed: 18523506
  4. Haleem AM, Singergy AA, Sabry D, Atta HM, Rashed LA, Chu CR, El Shewy MT, Azzam A, Abdel Aziz MT. The Clinical Use of Human Culture-Expanded Autologous Bone Marrow Mesenchymal Stem Cells Transplanted on Platelet-Rich Fibrin Glue in the Treatment of Articular Cartilage Defects: A Pilot Study and Preliminary Results.. Cartilage 2010 Oct;1(4):253-261.
    pmc: PMC3002255pubmed: 21170288doi: 10.1177/1947603510366027google scholar: lookup
  5. Giannini S, Buda R, Battaglia M, Cavallo M, Ruffilli A, Ramponi L, Pagliazzi G, Vannini F. One-step repair in talar osteochondral lesions: 4-year clinical results and t2-mapping capability in outcome prediction.. Am J Sports Med 2013 Mar;41(3):511-8.
    pubmed: 23221772doi: 10.1177/0363546512467622google scholar: lookup
  6. Centeno CJ, Schultz JR, Cheever M, Freeman M, Faulkner S, Robinson B, Hanson R. Safety and complications reporting update on the re-implantation of culture-expanded mesenchymal stem cells using autologous platelet lysate technique.. Curr Stem Cell Res Ther 2011 Dec;6(4):368-78.
    pubmed: 22023622doi: 10.2174/157488811797904371google scholar: lookup
  7. Wakitani S, Okabe T, Horibe S, Mitsuoka T, Saito M, Koyama T, Nawata M, Tensho K, Kato H, Uematsu K, Kuroda R, Kurosaka M, Yoshiya S, Hattori K, Ohgushi H. Safety of autologous bone marrow-derived mesenchymal stem cell transplantation for cartilage repair in 41 patients with 45 joints followed for up to 11 years and 5 months.. J Tissue Eng Regen Med 2011 Feb;5(2):146-50.
    pubmed: 20603892doi: 10.1002/term.299google scholar: lookup
  8. Fortier LA, Potter HG, Rickey EJ, Schnabel LV, Foo LF, Chong LR, Stokol T, Cheetham J, Nixon AJ. Concentrated bone marrow aspirate improves full-thickness cartilage repair compared with microfracture in the equine model.. J Bone Joint Surg Am 2010 Aug 18;92(10):1927-37.
    pubmed: 20720135doi: 10.2106/JBJS.I.01284google scholar: lookup
  9. Wilke MM, Nydam DV, Nixon AJ. Enhanced early chondrogenesis in articular defects following arthroscopic mesenchymal stem cell implantation in an equine model.. J Orthop Res 2007 Jul;25(7):913-25.
    pubmed: 17405160doi: 10.1002/jor.20382google scholar: lookup
  10. Hui TY, Cheung KM, Cheung WL, Chan D, Chan BP. In vitro chondrogenic differentiation of human mesenchymal stem cells in collagen microspheres: influence of cell seeding density and collagen concentration.. Biomaterials 2008 Aug;29(22):3201-12.
    pubmed: 18462789doi: 10.1016/j.biomaterials.2008.04.001google scholar: lookup
  11. Pastides P, Chimutengwende-Gordon M, Maffulli N, Khan W. Stem cell therapy for human cartilage defects: a systematic review.. Osteoarthritis Cartilage 2013 May;21(5):646-54.
    pubmed: 23485933doi: 10.1016/j.joca.2013.02.008google scholar: lookup
  12. van Buul GM, Koevoet WL, Kops N, Bos PK, Verhaar JA, Weinans H, Bernsen MR, van Osch GJ. Platelet-rich plasma releasate inhibits inflammatory processes in osteoarthritic chondrocytes.. Am J Sports Med 2011 Nov;39(11):2362-70.
    pubmed: 21856929doi: 10.1177/0363546511419278google scholar: lookup
  13. Lacci KM, Dardik A. Platelet-rich plasma: support for its use in wound healing.. Yale J Biol Med 2010 Mar;83(1):1-9.
    pmc: PMC2844688pubmed: 20351977
  14. Pagnotto MR, Wang Z, Karpie JC, Ferretti M, Xiao X, Chu CR. Adeno-associated viral gene transfer of transforming growth factor-beta1 to human mesenchymal stem cells improves cartilage repair.. Gene Ther 2007 May;14(10):804-13.
    pubmed: 17344902doi: 10.1038/sj.gt.3302938google scholar: lookup
  15. Smyth NA, Murawski CD, Fortier LA, Cole BJ, Kennedy JG. Platelet-rich plasma in the pathologic processes of cartilage: review of basic science evidence.. Arthroscopy 2013 Aug;29(8):1399-409.
    pubmed: 23669235doi: 10.1016/j.arthro.2013.03.004google scholar: lookup
  16. Giannini S, Buda R, Cavallo M, Ruffilli A, Cenacchi A, Cavallo C, Vannini F. Cartilage repair evolution in post-traumatic osteochondral lesions of the talus: from open field autologous chondrocyte to bone-marrow-derived cells transplantation.. Injury 2010 Nov;41(11):1196-203.
    pubmed: 20934692doi: 10.1016/j.injury.2010.09.028google scholar: lookup
  17. Buda R, Vannini F, Cavallo M, Grigolo B, Cenacchi A, Giannini S. Osteochondral lesions of the knee: a new one-step repair technique with bone-marrow-derived cells.. J Bone Joint Surg Am 2010 Dec;92 Suppl 2:2-11.
    pubmed: 21123588doi: 10.2106/JBJS.J.00813google scholar: lookup
  18. Kisiday JD, Goodrich LR, McIlwraith CW, Frisbie DD. Effects of equine bone marrow aspirate volume on isolation, proliferation, and differentiation potential of mesenchymal stem cells.. Am J Vet Res 2013 May;74(5):801-7.
    pubmed: 23627395doi: 10.2460/ajvr.74.5.801google scholar: lookup
  19. Hale BW, Goodrich LR, Frisbie DD, McIlwraith CW, Kisiday JD. Effect of scaffold dilution on migration of mesenchymal stem cells from fibrin hydrogels.. Am J Vet Res 2012 Feb;73(2):313-8.
    pubmed: 22280396doi: 10.2460/ajvr.73.2.313google scholar: lookup
  20. Goodrich LR, Hidaka C, Robbins PD, Evans CH, Nixon AJ. Genetic modification of chondrocytes with insulin-like growth factor-1 enhances cartilage healing in an equine model.. J Bone Joint Surg Br 2007 May;89(5):672-85.
    pubmed: 17540757doi: 10.1302/0301-620X.89B5.18343google scholar: lookup
  21. Xu X, Shi D, Shen Y, Xu Z, Dai J, Chen D, Teng H, Jiang Q. Full-thickness cartilage defects are repaired via a microfracture technique and intraarticular injection of the small-molecule compound kartogenin.. Arthritis Res Ther 2015 Feb 2;17(1):20.
    pmc: PMC4376363pubmed: 25641548doi: 10.1186/s13075-015-0537-1google scholar: lookup
  22. Nieminen MT, Rieppo J, Töyräs J, Hakumäki JM, Silvennoinen J, Hyttinen MM, Helminen HJ, Jurvelin JS. T2 relaxation reveals spatial collagen architecture in articular cartilage: a comparative quantitative MRI and polarized light microscopic study.. Magn Reson Med 2001 Sep;46(3):487-93.
    pubmed: 11550240doi: 10.1002/mrm.1218google scholar: lookup
  23. Miller RE, Grodzinsky AJ, Barrett MF, Hung HH, Frank EH, Werpy NM, McIlwraith CW, Frisbie DD. Effects of the combination of microfracture and self-assembling Peptide filling on the repair of a clinically relevant trochlear defect in an equine model.. J Bone Joint Surg Am 2014 Oct 1;96(19):1601-9.
    pmc: PMC4179451pubmed: 25274785doi: 10.2106/JBJS.M.01408google scholar: lookup
  24. Pallante AL, Görtz S, Chen AC, Healey RM, Chase DC, Ball ST, Amiel D, Sah RL, Bugbee WD. Treatment of articular cartilage defects in the goat with frozen versus fresh osteochondral allografts: effects on cartilage stiffness, zonal composition, and structure at six months.. J Bone Joint Surg Am 2012 Nov 7;94(21):1984-95.
    pmc: PMC3489067pubmed: 23138239doi: 10.2106/JBJS.K.00439google scholar: lookup
  25. Chan EF, Liu IL, Semler EJ, Aberman HM, Simon TM, Chen AC, Truncale KG, Sah RL. Association of 3-Dimensional Cartilage and Bone Structure with Articular Cartilage Properties in and Adjacent to Autologous Osteochondral Grafts after 6 and 12 months in a Goat Model.. Cartilage 2012 Jul 1;3(3):255-66.
    pmc: PMC3818730pubmed: 24224069doi: 10.1177/1947603511435272google scholar: lookup
  26. Palmer AW, Guldberg RE, Levenston ME. Analysis of cartilage matrix fixed charge density and three-dimensional morphology via contrast-enhanced microcomputed tomography.. Proc Natl Acad Sci U S A 2006 Dec 19;103(51):19255-60.
    pmc: PMC1748213pubmed: 17158799doi: 10.1073/pnas.0606406103google scholar: lookup
  27. Frisbie DD, Lu Y, Kawcak CE, DiCarlo EF, Binette F, McIlwraith CW. In vivo evaluation of autologous cartilage fragment-loaded scaffolds implanted into equine articular defects and compared with autologous chondrocyte implantation.. Am J Sports Med 2009 Nov;37 Suppl 1:71S-80S.
    pubmed: 19934439doi: 10.1177/0363546509348478google scholar: lookup
  28. Hidaka C, Goodrich LR, Chen CT, Warren RF, Crystal RG, Nixon AJ. Acceleration of cartilage repair by genetically modified chondrocytes over expressing bone morphogenetic protein-7.. J Orthop Res 2003 Jul;21(4):573-83.
    pubmed: 12798054doi: 10.1016/S0736-0266(02)00264-4google scholar: lookup
  29. Frisbie DD, Bowman SM, Colhoun HA, DiCarlo EF, Kawcak CE, McIlwraith CW. Evaluation of autologous chondrocyte transplantation via a collagen membrane in equine articular defects: results at 12 and 18 months.. Osteoarthritis Cartilage 2008 Jun;16(6):667-79.
    pubmed: 18042409doi: 10.1016/j.joca.2007.09.013google scholar: lookup
  30. Lee JC, Min HJ, Park HJ, Lee S, Seong SC, Lee MC. Synovial membrane-derived mesenchymal stem cells supported by platelet-rich plasma can repair osteochondral defects in a rabbit model.. Arthroscopy 2013 Jun;29(6):1034-46.
    pubmed: 23726109doi: 10.1016/j.arthro.2013.02.026google scholar: lookup
  31. Park SI, Lee HR, Kim S, Ahn MW, Do SH. Time-sequential modulation in expression of growth factors from platelet-rich plasma (PRP) on the chondrocyte cultures.. Mol Cell Biochem 2012 Feb;361(1-2):9-17.
    pubmed: 21956670doi: 10.1007/s11010-011-1081-1google scholar: lookup
  32. Milano G, Deriu L, Sanna Passino E, Masala G, Manunta A, Postacchini R, Saccomanno MF, Fabbriciani C. Repeated platelet concentrate injections enhance reparative response of microfractures in the treatment of chondral defects of the knee: an experimental study in an animal model.. Arthroscopy 2012 May;28(5):688-701.
    pubmed: 22277762doi: 10.1016/j.arthro.2011.09.016google scholar: lookup
  33. Lee HR, Park KM, Joung YK, Park KD, Do SH. Platelet-rich plasma loaded hydrogel scaffold enhances chondrogenic differentiation and maturation with up-regulation of CB1 and CB2.. J Control Release 2012 May 10;159(3):332-7.
    pubmed: 22366523doi: 10.1016/j.jconrel.2012.02.008google scholar: lookup
  34. Sun Y, Feng Y, Zhang CQ, Chen SB, Cheng XG. The regenerative effect of platelet-rich plasma on healing in large osteochondral defects.. Int Orthop 2010 Apr;34(4):589-97.
    pmc: PMC2903145pubmed: 19434411doi: 10.1007/s00264-009-0793-2google scholar: lookup
  35. Qi YY, Chen X, Jiang YZ, Cai HX, Wang LL, Song XH, Zou XH, Ouyang HW. Local delivery of autologous platelet in collagen matrix simulated in situ articular cartilage repair.. Cell Transplant 2009;18(10):1161-9.
    pubmed: 19660173doi: 10.3727/096368909X12483162197169google scholar: lookup
  36. Perut F, Filardo G, Mariani E, Cenacchi A, Pratelli L, Devescovi V, Kon E, Marcacci M, Facchini A, Baldini N, Granchi D. Preparation method and growth factor content of platelet concentrate influence the osteogenic differentiation of bone marrow stromal cells.. Cytotherapy 2013 Jul;15(7):830-9.
    pubmed: 23731763doi: 10.1016/j.jcyt.2013.01.220google scholar: lookup
  37. Ronca A, Guarino V, Raucci MG, Salamanna F, Martini L, Zeppetelli S, Fini M, Kon E, Filardo G, Marcacci M, Ambrosio L. Large defect-tailored composite scaffolds for in vivo bone regeneration.. J Biomater Appl 2014 Nov;29(5):715-27.
    pubmed: 24951457doi: 10.1177/0885328214539823google scholar: lookup
  38. Lee DH, Ryu KJ, Kim JW, Kang KC, Choi YR. Bone marrow aspirate concentrate and platelet-rich plasma enhanced bone healing in distraction osteogenesis of the tibia.. Clin Orthop Relat Res 2014 Dec;472(12):3789-97.
    pmc: PMC4397746pubmed: 24599650doi: 10.1007/s11999-014-3548-3google scholar: lookup
  39. Stolzing A, Colley H, Scutt A. Effect of age and diabetes on the response of mesenchymal progenitor cells to fibrin matrices.. Int J Biomater 2011;2011:378034.
    pmc: PMC3238389pubmed: 22194749doi: 10.1155/2011/378034google scholar: lookup
  40. Lee HH, Haleem AM, Yao V, Li J, Xiao X, Chu CR. Release of bioactive adeno-associated virus from fibrin scaffolds: effects of fibrin glue concentrations.. Tissue Eng Part A 2011 Aug;17(15-16):1969-78.
    pmc: PMC3142631pubmed: 21449684doi: 10.1089/ten.TEA.2010.0586google scholar: lookup
  41. Fritz J, Janssen P, Gaissmaier C, Schewe B, Weise K. Articular cartilage defects in the knee--basics, therapies and results.. Injury 2008 Apr;39 Suppl 1:S50-7.
    pubmed: 18313472doi: 10.1016/j.injury.2008.01.039google scholar: lookup
  42. Ahmed TA, Hincke MT. Strategies for articular cartilage lesion repair and functional restoration.. Tissue Eng Part B Rev 2010 Jun;16(3):305-29.
    pubmed: 20025455doi: 10.1089/ten.TEB.2009.0590google scholar: lookup
  43. Frisbie DD, McCarthy HE, Archer CW, Barrett MF, McIlwraith CW. Evaluation of articular cartilage progenitor cells for the repair of articular defects in an equine model.. J Bone Joint Surg Am 2015 Mar 18;97(6):484-93.
    pubmed: 25788305doi: 10.2106/JBJS.N.00404google scholar: lookup
  44. Gelber PE, Batista J, Millan-Billi A, Patthauer L, Vera S, Gomez-Masdeu M, Monllau JC. Magnetic resonance evaluation of TruFit® plugs for the treatment of osteochondral lesions of the knee shows the poor characteristics of the repair tissue.. Knee 2014 Aug;21(4):827-32.
    pubmed: 24856089doi: 10.1016/j.knee.2014.04.013google scholar: lookup
  45. McMahon LA, O'Brien FJ, Prendergast PJ. Biomechanics and mechanobiology in osteochondral tissues.. Regen Med 2008 Sep;3(5):743-59.
    pubmed: 18729798doi: 10.2217/17460751.3.5.743google scholar: lookup
  46. Moran CJ, Pascual-Garrido C, Chubinskaya S, Potter HG, Warren RF, Cole BJ, Rodeo SA. Restoration of articular cartilage.. J Bone Joint Surg Am 2014 Feb 19;96(4):336-44.
    pubmed: 24553893doi: 10.2106/JBJS.L.01329google scholar: lookup
  47. Perdisa F, Filardo G, Di Matteo B, Marcacci M, Kon E. Platelet rich plasma: a valid augmentation for cartilage scaffolds? A systematic review.. Histol Histopathol 2014 Jul;29(7):805-14.
    pubmed: 24458849doi: 10.14670/HH-29.805google scholar: lookup
  48. Dold AP, Zywiel MG, Taylor DW, Dwyer T, Theodoropoulos J. Platelet-rich plasma in the management of articular cartilage pathology: a systematic review.. Clin J Sport Med 2014 Jan;24(1):31-43.
    pubmed: 24231930doi: 10.1097/01.jsm.0000432855.85143.e5google scholar: lookup
  49. Tadie J, Ann X. Comments on "Repeated platelet concentrate injections enhance reparative response of microfractures in the treatment of chondral defects of the knee: an experimental study in an animal model" by Milano et al.. Arthroscopy 2013 Oct;29(10):1599.
    pubmed: 24075607doi: 10.1016/j.arthro.2013.07.267google scholar: lookup
  50. Siclari A, Mascaro G, Gentili C, Kaps C, Cancedda R, Boux E. Cartilage repair in the knee with subchondral drilling augmented with a platelet-rich plasma-immersed polymer-based implant.. Knee Surg Sports Traumatol Arthrosc 2014 Jun;22(6):1225-34.
    pubmed: 23563814doi: 10.1007/s00167-013-2484-1google scholar: lookup

Citations

This article has been cited 25 times.
  1. Lee E, Epanomeritakis IE, Lu V, Khan W. Bone Marrow-Derived Mesenchymal Stem Cell Implants for the Treatment of Focal Chondral Defects of the Knee in Animal Models: A Systematic Review and Meta-Analysis. Int J Mol Sci 2023 Feb 6;24(4).
    doi: 10.3390/ijms24043227pubmed: 36834639google scholar: lookup
  2. Rojas-Murillo JA, Simental-Mendía MA, Moncada-Saucedo NK, Delgado-Gonzalez P, Islas JF, Roacho-Pérez JA, Garza-Treviño EN. Physical, Mechanical, and Biological Properties of Fibrin Scaffolds for Cartilage Repair. Int J Mol Sci 2022 Aug 30;23(17).
    doi: 10.3390/ijms23179879pubmed: 36077276google scholar: lookup
  3. Estrada McDermott J, Pezzanite L, Goodrich L, Santangelo K, Chow L, Dow S, Wheat W. Role of Innate Immunity in Initiation and Progression of Osteoarthritis, with Emphasis on Horses. Animals (Basel) 2021 Nov 13;11(11).
    doi: 10.3390/ani11113247pubmed: 34827979google scholar: lookup
  4. Velloso Alvarez A, Boone LH, Braim AP, Taintor JS, Caldwell F, Wright JC, Wooldridge AA. A Survey of Clinical Usage of Non-steroidal Intra-Articular Therapeutics by Equine Practitioners. Front Vet Sci 2020;7:579967.
    doi: 10.3389/fvets.2020.579967pubmed: 33195592google scholar: lookup
  5. Wu J, Chen Q, Deng C, Xu B, Zhang Z, Yang Y, Lu T. Exquisite design of injectable Hydrogels in Cartilage Repair. Theranostics 2020;10(21):9843-9864.
    doi: 10.7150/thno.46450pubmed: 32863963google scholar: lookup
  6. Fugazzola MC, van Weeren PR. Surgical osteochondral defect repair in the horse-a matter of form or function?. Equine Vet J 2020 Jul;52(4):489-499.
    doi: 10.1111/evj.13231pubmed: 31958175google scholar: lookup
  7. Gao X, Cheng H, Awada H, Tang Y, Amra S, Lu A, Sun X, Lv G, Huard C, Wang B, Bi X, Wang Y, Huard J. A comparison of BMP2 delivery by coacervate and gene therapy for promoting human muscle-derived stem cell-mediated articular cartilage repair. Stem Cell Res Ther 2019 Nov 26;10(1):346.
    doi: 10.1186/s13287-019-1434-3pubmed: 31771623google scholar: lookup
  8. Irwin RM, Bonassar LJ, Cohen I, Matuska AM, Commins J, Cole B, Fortier LA. The clot thickens: Autologous and allogeneic fibrin sealants are mechanically equivalent in an ex vivo model of cartilage repair. PLoS One 2019;14(11):e0224756.
    doi: 10.1371/journal.pone.0224756pubmed: 31703078google scholar: lookup
  9. Gugjoo MB, Fazili MR, Gayas MA, Ahmad RA, Dhama K. Animal mesenchymal stem cell research in cartilage regenerative medicine - a review. Vet Q 2019 Dec;39(1):95-120.
    doi: 10.1080/01652176.2019.1643051pubmed: 31291836google scholar: lookup
  10. Barbon S, Stocco E, Macchi V, Contran M, Grandi F, Borean A, Parnigotto PP, Porzionato A, De Caro R. Platelet-Rich Fibrin Scaffolds for Cartilage and Tendon Regenerative Medicine: From Bench to Bedside. Int J Mol Sci 2019 Apr 5;20(7).
    doi: 10.3390/ijms20071701pubmed: 30959772google scholar: lookup
  11. Hur CI, Ahn HW, Seon JK, Song EK, Kim GE. Mesenchymal Stem Cells Decrease Tunnel Widening of Anterior Cruciate Ligament Reconstruction in Rabbit Model. Int J Stem Cells 2019 Mar 30;12(1):162-169.
    doi: 10.15283/ijsc18022pubmed: 30595005google scholar: lookup
  12. Cengiz IF, Oliveira JM, Reis RL. Micro-CT - a digital 3D microstructural voyage into scaffolds: a systematic review of the reported methods and results. Biomater Res 2018;22:26.
    doi: 10.1186/s40824-018-0136-8pubmed: 30275969google scholar: lookup
  13. Bogers SH. Cell-Based Therapies for Joint Disease in Veterinary Medicine: What We Have Learned and What We Need to Know. Front Vet Sci 2018;5:70.
    doi: 10.3389/fvets.2018.00070pubmed: 29713634google scholar: lookup
  14. Ball AN, Donahue SW, Wojda SJ, McIlwraith CW, Kawcak CE, Ehrhart N, Goodrich LR. The challenges of promoting osteogenesis in segmental bone defects and osteoporosis. J Orthop Res 2018 Jun;36(6):1559-1572.
    doi: 10.1002/jor.23845pubmed: 29280510google scholar: lookup
  15. Cotter EJ, Wang KC, Yanke AB, Chubinskaya S. Bone Marrow Aspirate Concentrate for Cartilage Defects of the Knee: From Bench to Bedside Evidence. Cartilage 2018 Apr;9(2):161-170.
    doi: 10.1177/1947603517741169pubmed: 29126349google scholar: lookup
  16. Pascual-Garrido C, Rodriguez-Fontan F, Aisenbrey EA, Payne KA, Chahla J, Goodrich LR, Bryant SJ. Current and novel injectable hydrogels to treat focal chondral lesions: Properties and applicability. J Orthop Res 2018 Jan;36(1):64-75.
    doi: 10.1002/jor.23760pubmed: 28975658google scholar: lookup
  17. Mancini IAD, Vindas Bolaños RA, Brommer H, Castilho M, Ribeiro A, van Loon JPAM, Mensinga A, van Rijen MHP, Malda J, van Weeren R. Fixation of Hydrogel Constructs for Cartilage Repair in the Equine Model: A Challenging Issue. Tissue Eng Part C Methods 2017 Nov;23(11):804-814.
    doi: 10.1089/ten.TEC.2017.0200pubmed: 28795641google scholar: lookup
  18. Ikeda Y, Sakaue M, Chijimatsu R, Hart DA, Otsubo H, Shimomura K, Madry H, Suzuki T, Yoshikawa H, Yamashita T, Nakamura N. IGF-1 Gene Transfer to Human Synovial MSCs Promotes Their Chondrogenic Differentiation Potential without Induction of the Hypertrophic Phenotype. Stem Cells Int 2017;2017:5804147.
    doi: 10.1155/2017/5804147pubmed: 28740513google scholar: lookup
  19. Berebichez-Fridman R, Gómez-García R, Granados-Montiel J, Berebichez-Fastlicht E, Olivos-Meza A, Granados J, Velasquillo C, Ibarra C. The Holy Grail of Orthopedic Surgery: Mesenchymal Stem Cells-Their Current Uses and Potential Applications. Stem Cells Int 2017;2017:2638305.
    doi: 10.1155/2017/2638305pubmed: 28698718google scholar: lookup
  20. Kraeutler MJ, Mitchell JJ, Chahla J, McCarty EC, Pascual-Garrido C. Intra-articular Implantation of Mesenchymal Stem Cells, Part 1: A Review of the Literature for Prevention of Postmeniscectomy Osteoarthritis. Orthop J Sports Med 2017 Jan;5(1):2325967116680815.
    doi: 10.1177/2325967116680815pubmed: 28203597google scholar: lookup
  21. Mirza MH, Bommala P, Richbourg HA, Rademacher N, Kearney MT, Lopez MJ. Gait Changes Vary among Horses with Naturally Occurring Osteoarthritis Following Intra-articular Administration of Autologous Platelet-Rich Plasma. Front Vet Sci 2016;3:29.
    doi: 10.3389/fvets.2016.00029pubmed: 27148544google scholar: lookup
  22. Yang C, Chen R, Chen C, Yang F, Xiao H, Geng B, Xia Y. Tissue engineering strategies hold promise for the repair of articular cartilage injury. Biomed Eng Online 2024 Sep 11;23(1):92.
    doi: 10.1186/s12938-024-01260-wpubmed: 39261876google scholar: lookup
  23. Yamada M, Nakajima A, Sakurai K, Tamada Y, Nakagawa K. Cell Proliferation, Chondrogenic Differentiation, and Cartilaginous Tissue Formation in Recombinant Silk Fibroin with Basic Fibroblast Growth Factor Binding Peptide. J Funct Biomater 2024 Aug 17;15(8).
    doi: 10.3390/jfb15080230pubmed: 39194668google scholar: lookup
  24. Campbell TM, Trudel G. Protecting the regenerative environment: selecting the optimal delivery vehicle for cartilage repair-a narrative review. Front Bioeng Biotechnol 2024;12:1283752.
    doi: 10.3389/fbioe.2024.1283752pubmed: 38333081google scholar: lookup
  25. Chu CR, Williams AA, Erhart-Hledik JC, Titchenal MR, Qian Y, Andriacchi TP. Visualizing pre-osteoarthritis: Integrating MRI UTE-T2* with mechanics and biology to combat osteoarthritis-The 2019 Elizabeth Winston Lanier Kappa Delta Award. J Orthop Res 2021 Aug;39(8):1585-1595.
    doi: 10.1002/jor.25045pubmed: 33788306google scholar: lookup
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