Adhesion and proliferation of human dermal fibroblasts on collagen matrix.
Abstract: The purpose of this study was to evaluate adhesion and growth of human dermal fibroblasts on a 0.150 mm-thick matrix of reconstituted collagen isolated from horse tendon. Collagen was extracted and polymerized according to the standard procedures (Opocrin, Corlo, Modena, Italy). By light microscopy, the bottom surface of the matrix appeared linear and compact, whereas the superficial one was indented and less homogeneous. By scanning electron microscopy, the collagen fibrils had different diameters and the great majority of them was oriented parallel to the surface of the gel. By transmission electron microscopy, collagen fibrils showed the typical banding. Human dermal fibroblasts were seeded on the collagen matrix, previously equilibrated in growth medium. Fibroblast proliferation stopped in the second week and was always significantly lower than that of the same cell strain seeded on plastic and cultured in parallel. By light microscopy, after six days culture, cells formed a confluent multilayer on the surface of the gel. By scanning and transmission electron microscopy, fibroblasts appeared flat and adherent to the matrix. Contacts of cells among themselves and with the collagen fibrils were observed. Fibroblasts never moved into the collagen gel. In conclusion, human dermal fibroblasts can be grown in a three-dimensional matrix made by horse tendon that, on the other hand, seems to condition their proliferation rate.
Publication Date: 2004-02-12 PubMed ID: 14871046DOI: 10.1177/0885328204039692Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research study explored how human dermal fibroblasts adhere and grow on a collagen matrix from horse tendons. The results showed that human dermal fibroblasts could indeed be grown in such a matrix, albeit at a lower proliferation rate.
Experimental Design
- The researchers used a 0.150 mm-thick matrix of reconstituted collagen extracted from horse tendons. The extraction and polymerization of collagen followed the standard procedures.
- Human dermal fibroblasts, which are the most common cells of connective tissue, were then seeded on the collagen matrix that had been equilibrated in growth medium.
Methodology and Observations
- Light microscopy was used to observe that the bottom surface of the matrix was linear and compact, whilst the superficial surface presented as indented and less homogeneous.
- With scanning electron microscopy, they found that the collagen fibrils had varying diameters, and most were oriented parallel to the surface of the gel. The fibrils displayed typical banding when viewed through transmission electron microscopy.
- Light microscopy also found a confluent multilayer of cells on the surface of the gel after six days of culture. When viewed under scanning and transmission electron microscopy, fibroblasts appeared flat and adherent to the matrix.
Results and Conclusion
- Fibroblast proliferation, which is the process of cell division that results in cell growth, stopped during the second week. The proliferation rate was significantly lower than that of the same cell strain seeded on plastic and cultured in parallel.
- Cells formed contacts among themselves and with the collagen fibrils, but they never moved into the collagen gel.
- Based on their findings, the researchers concluded that while human dermal fibroblasts can indeed be grown in a three-dimensional matrix made by horse tendon, this matrix appears to condition, or lower, their proliferation rate.
Cite This Article
APA
Croce MA, Silvestri C, Guerra D, Carnevali E, Boraldi F, Tiozzo R, Parma B.
(2004).
Adhesion and proliferation of human dermal fibroblasts on collagen matrix.
J Biomater Appl, 18(3), 209-222.
https://doi.org/10.1177/0885328204039692 Publication
Researcher Affiliations
- Department of Biomedical Sciences, University of Modena and Reggio Emilia, Via G. Campi 287, 41100 Modena, Italy.
MeSH Terms
- Animals
- Cell Adhesion
- Cell Division
- Collagen
- Fibroblasts / cytology
- Fibroblasts / ultrastructure
- Horses
- Humans
- Microscopy, Confocal
- Microscopy, Electron
- Skin / cytology
- Skin / ultrastructure
Citations
This article has been cited 7 times.- Gallo N, Natali ML, Quarta A, Gaballo A, Terzi A, Sibillano T, Giannini C, De Benedetto GE, Lunetti P, Capobianco L, Blasi FS, Sicuro A, Corallo A, Sannino A, Salvatore L. Aquaponics-Derived Tilapia Skin Collagen for Biomaterials Development. Polymers (Basel) 2022 May 2;14(9).
- Gallo N, Natali ML, Curci C, Picerno A, Gallone A, Vulpi M, Vitarelli A, Ditonno P, Cascione M, Sallustio F, Rinaldi R, Sannino A, Salvatore L. Analysis of the Physico-Chemical, Mechanical and Biological Properties of Crosslinked Type-I Collagen from Horse Tendon: Towards the Development of Ideal Scaffolding Material for Urethral Regeneration. Materials (Basel) 2021 Dec 12;14(24).
- Gallo N, Natali ML, Sannino A, Salvatore L. An Overview of the Use of Equine Collagen as Emerging Material for Biomedical Applications. J Funct Biomater 2020 Nov 1;11(4).
- van Vijven M, Wunderli SL, Ito K, Snedeker JG, Foolen J. Serum deprivation limits loss and promotes recovery of tenogenic phenotype in tendon cell culture systems. J Orthop Res 2021 Jul;39(7):1561-1571.
- Tiago M, de Oliveira EM, Brohem CA, Pennacchi PC, Paes RD, Haga RB, Campa A, de Moraes Barros SB, Smalley KS, Maria-Engler SS. Fibroblasts protect melanoma cells from the cytotoxic effects of doxorubicin. Tissue Eng Part A 2014 Sep;20(17-18):2412-21.
- Wagenhäuser MU, Pietschmann MF, Docheva D, Gülecyüz MF, Jansson V, Müller PE. Assessment of essential characteristics of two different scaffolds for tendon in situ regeneration. Knee Surg Sports Traumatol Arthrosc 2015 Apr;23(4):1239-46.
- Popescu S, Demetrescu I, Sarantopoulos C, Gleizes AN, Iordachescu D. The biocompatibility of titanium in a buffer solution: compared effects of a thin film of TiO2 deposited by MOCVD and of collagen deposited from a gel. J Mater Sci Mater Med 2007 Oct;18(10):2075-83.
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