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Scientific reports2023; 13(1); 21824; doi: 10.1038/s41598-023-48933-5

Advanced platelet-rich fibrin promotes healing of induced corneal ulcer in donkeys (Equus asinus).

Abstract: Ulcerative keratitis is a common disease in horses which may cause blindness. To prevent secondary bacterial and fungal infections and promote quick re-growth of the epithelial layer, different treatment approaches have been employed. This study aimed to examine the effects of advanced platelet-rich fibrin (A-PRF) gel on the healing process of experimentally induced corneal ulcers in donkeys. Nine healthy adult donkeys were used for the study. The donkeys were divided into two groups: the control group, where no medication was applied to the corneal ulcer, and the A-PRF gel group, where A-PRF gel was applied once a day on specific days after ulcer induction. The healing process was evaluated through various examinations and analyses. The results demonstrated that the A-PRF gel group showed significant improvement in the corneal ulcer area, with epithelial and stromal regeneration. At day 35, about 60% of the A-PRF group showed negative fluorescein uptake. Additionally, fewer complications were observed during the healing process compared to the control group. In conclusion, A-PRF gel is an important and safe therapeutic option for controlling ocular surface infection and promoting corneal healing. We recommend using A-PRF gel as an alternative approach, avoiding eyelid suturing, and minimizing corneal irritation.
© 2023. The Author(s).
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Publication Date: 2023-12-09 PubMed ID: 38071242PubMed Central: 10502137DOI: 10.1038/s41598-023-48933-5Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research aimed at investigating the effectiveness of an advanced platelet-rich fibrin (A-PRF) gel in enhancing the healing process of experimentally induced corneal ulcers in donkeys, showing that the treatment promoted a marked improvement in healing and reduced complications.

Study Design

  • Nine healthy adult donkeys were chosen for this experimental research. A controlled design was used, with the animals split into two groups for the purpose of comparative analysis.
  • The control group did not receive any medication on their induced corneal ulcers. The purpose of this group was to naturally observe the progression and healing process of an untreated corneal ulcer.
  • For the second group, A-PRF gel was applied on their corneal ulcers once a day on specific days after the ulcer was induced. The intention here was to comprehend how the application of A-PRF gel affected the healing process of a corneal ulcer.
  • Throughout the experiment duration, the healing process of the ulcers in each group was closely monitored, evaluated, and compared. Various examinations and analyses were carried out for detailed data.

Results

  • The results of the experiment showed a significant improvement in the corneal ulcer area of the group that received A-PRF gel. This was noted with both an improvement in epithelial regeneration–naturally occurring repair of tissue, and stromal regeneration–the healing of the thick, supportive layer of the cornea.
  • The A-PRF gel also resulted in faster healing rates. By day 35 of the experiment, about 60% of the donkeys treated with A-PRF gel showed no signs of fluorescein uptake, indicating an absence of corneal defects. This is compared to the control group where, presumably, ulcers were still present.
  • The study also denoted fewer complications during the healing process in the A-PRF treated group when contrasted with the control group.

Conclusion

  • In conclusion, the study determined that A-PRF gel is a safe and effective therapeutic option for controlling ocular surface infections and promoting corneal healing in cases of ulcerative keratitis.
  • The research recommends the use of A-PRF gel as an alternative approach to more conventional treatments. The study also suggests A-PRF could lessen the need for eyelid suturing and minimize corneal irritation during treatment.

Cite This Article

APA
Hosny OH, Abd-Elkareem M, Ali MM, Ahmed AF. (2023). Advanced platelet-rich fibrin promotes healing of induced corneal ulcer in donkeys (Equus asinus). Sci Rep, 13(1), 21824. https://doi.org/10.1038/s41598-023-48933-5

Publication

Scientific reports
ISSN: 2045-2322
NlmUniqueID: 101563288
Country: England
Language: English
Volume: 13
Issue: 1
Pages: 21824

Researcher Affiliations

Hosny, Omar H
  • Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Assiut University, Assiut, 71526, Egypt. omarhussein@aun.edu.eg.
Abd-Elkareem, Mahmoud
  • Department of Cell and Tissue, Faculty of Veterinary Medicine, Assiut University, Assiut, 71526, Egypt.
Ali, Magda M
  • Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Assiut University, Assiut, 71526, Egypt.
Ahmed, Ahmed F
  • Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Assiut University, Assiut, 71526, Egypt.

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