Agreement between hematocrit values determined by the Cobas b121 blood gas analyzer and the microhematocrit method in dogs, cats, and horses.
Abstract: Packed cell volume (PCV) is important for assessing a patient's health status. Some blood gas analyzers measure hematocrit, and the agreement with PCV varies among different analyzers. Objective: We aimed to determine the agreement between PCV measured by microcentrifugation and hematocrit measured by the Cobas b121 blood gas analyzer in dogs, cats, and horses. Methods: Whole blood samples for PCV and blood gas analysis were collected in lithium-heparin syringes and analyzed within 10 min of collection. Agreement and association between the PCV and Cobas b121 generated hematocrit were assessed by the Bland-Altman method, Pearson's correlation, Deming regression analysis, and paired t tests. A total allowable error of 10% was used for the analysis. Results: This study included 45 dogs, 45 cats, and 33 horses. The respective mean ± SD (minimum-maximum) of PCVs and hematocrits were: dogs, 34.9 ± 9.9% (9.0-55.0) and 32.5 ± 8.8% (10.4-50.6); cats, 29.0 ± 9.6% (11.0-51.0) and 26.9 ± 9.3% (10.2-50.9); horses, 34.2 ± 6.5% (24.0-47.0) and 34.1 ± 6.0% (22.5-46.1). There were no significant differences between the methods. The bias ± SD was: dogs, -2.4 ± 2.6%; cats, -2.2 ± 2.3%; horses, -0.1 ± 2.4%. Pearson's correlation coefficients were > 0.90 for all species (P < 0.0001). In 60%, 49%, and 85% of the samples for dogs, cats, and horses, respectively, the percentage differences between the methods were within 10%. Conclusions: The Cobas b121 blood gas analyzer provided accurate estimates of PCVs in horses. However, in dogs and cats, there was a large frequency of unacceptable differences between the methods.
© 2023 American Society for Veterinary Clinical Pathology.
Publication Date: 2023-07-12 PubMed ID: 37438319DOI: 10.1111/vcp.13245Google Scholar: Lookup
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Summary
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The research focuses on determining how closely hematocrit values calculated by the Cobas b121 blood gas analyzer matches with Packed Cell Volume (PCV) determined by microcentrifugation in cats, dogs, and horses. The Cobas b121 blood gas analyzer showed significant accuracy in estimating PCV in horses, but a high frequency of unacceptable differences was observed in cats and dogs.
Objective
- The primary objective of this research was to find out the level of agreement between the Packed Cell Volume (PCV) calculated through microcentrifugation and the PCV measured using the Cobas b121 blood gas analyzer in domestic animals including dogs, cats, and horses.
Methods
- Blood samples were collected from the subjects using lithium-heparin syringes.
- The samples were analyzed within 10 minutes of collection.
- The statistical methods used included the Bland-Altman method, Pearson’s correlation, Deming regression analysis, and paired t tests.
- The analytical accuracy was determined using a total allowance error of 10%.
Results
- The study included 45 dogs, 45 cats, and 33 horses. Various PCVs and hematocrits were determined where:
- for dogs, the mean was found to be 34.9 ± 9.9% (9.0-55.0) and 32.5 ± 8.8% (10.4-50.6),
- for cats, the mean was found to be 29.0 ± 9.6% (11.0-51.0) and 26.9 ± 9.3% (10.2-50.9),
- for horses, the mean was found to be 34.2 ± 6.5% (24.0-47.0) and 34.1 ± 6.0% (22.5-46.1).
- The bias was -2.4 ± 2.6% for dogs, -2.2 ± 2.3% for cats, and -0.1 ± 2.4% for horses.
- Pearson’s correlation coefficients were >0.90 for all species, implying a high level of correlation between the two methods.
- Percentage differences between the results of the two methods were within 10% for 60% of dogs, 49% of cats, and 85% of horses.
Conclusion
- In horses, the Cobas b121 blood gas analyzer was found to provide highly accurate estimates of PCVs.
- The level of agreement between the methods was less reliable for dogs and cats due to a high frequency of unacceptable differences.
Cite This Article
APA
Dallarosa P, Monteiro ER, Borenstein APS, Valle SF.
(2023).
Agreement between hematocrit values determined by the Cobas b121 blood gas analyzer and the microhematocrit method in dogs, cats, and horses.
Vet Clin Pathol.
https://doi.org/10.1111/vcp.13245 Publication
Researcher Affiliations
- Faculty of Veterinary Medicine, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
- Faculty of Veterinary Medicine, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
- Faculty of Veterinary Medicine, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
- Faculty of Veterinary Medicine, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
Grant Funding
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Universidade Federal do Rio Grande do Sul
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